Electrochemical competitive hybridization assay for DNA detection using osmium tetroxide-labelled signalling strands

Duwensee, Heiko; Jacobsen, Martin; Flechsig, Gerd‐Uwe

doi:10.1039/b819283a

Cited by 19 publications

(10 citation statements)

References 26 publications

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“…Compared to label-based electrochemical DNA detection studies, our simple work does not contain long experimental steps [35,36] expensive electrodes and oligonucleotides labelled with À SH group [31,35,36,42,55].…”

Section: Resultsmentioning

confidence: 99%

“…Panke et al [31] reported a competitive binding protocol and used methylene blue as electroactive label for the determination of DNA single base mismatches in combination with differential pulse voltammetry. Duwensee et al [42] presented sequence-specific DNA detection by means of a competitive hybridization assay with osmium tetroxide-labeled signaling probes and they could detected 25 nM of target DNA in 30 minutes time.…”

Section: Introductionmentioning

confidence: 99%

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An Electrochemical DNA Biosensor for the Detection of the Apa I Polymorphism in the Vitamin D Receptor Gene Using Meldola's Blue as a Hybridization Indicator

et al. 2010

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Electrochemical detection of nucleic acid base mismatches related to Apa I single nucleotide polymorphism (SNP) in the vitamin D receptor gene was performed successfully using 7-dimethyl-amino-1,2-benzophenoxazinium salt (Meldolas blue, MDB) with 10.9 pmol/100 mL of detection limit. MDB reduction signals obtained from probe, mismatch(probe-SNP containing target) and hybrid(probe-target) modified pencil graphite electrode(PGE) increased respectively. The sensor was able to clearly distinguish perfect match from mismatch DNA in a 30 min. detection time. Several factors affecting on the hybridization and indicator response are studied to maximize sensitivity and selectivity. The advantages of the biosensor are discussed in comparison with previous electrochemical assays for DNA hybridization.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An Electrochemical DNA Biosensor for the Detection of the Apa I Polymorphism in the Vitamin D Receptor Gene Using Meldola's Blue as a Hybridization Indicator

et al. 2010

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“…In the literature DNA sensors are reported with considerably lower detection limits [30, 37 -39], however the sensor described here does not use any amplification step and thus compares to other systems using direct redox label detection [40].…”

Section: Quantificationmentioning

confidence: 99%

Parallel Detection of Different DNA Sequences on One Gold Electrode

Bütow

Lisdat

2010

Electroanalysis

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Motivated by the potential of electrochemical techniques to analyze hybridization events fast and in a simple and costeffective way we present here a detection system allowing a parallel electrochemical DNA analysis. For this purpose different probe DNA strands have been immobilized on one electrode. By the use of two different target DNA sequences, both marked with the redox active methylene blue, we can show that hybridization with the complementary probe sh"NA strands can occur without steric hindrance. Each target has been recognized down to 3nM with a very high specificity of the sensor. In addition, we can detect two different ssDNA targets labeled with different redox active molecules, methylene blue and ferrocene, on one sensor surface simultaneously.

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“…Secondly, a reporter strand can be utilized that binds onto the target in addition and right beside to the capture probe [61]. Thirdly, in a competitive assay, a labeled signaling strand can be used that will be displaced by the target and therefore will lead to signal decrease ("switch off") upon hybridization with the target [62,80].…”

Section: Use Of Covalent Markersmentioning

confidence: 99%

Sequence-specific electrochemical detection of nucleic acids in real samples

Duwensee¹,

Mix

Flechsig

2010

Bioanal Rev

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This paper reviews past and current developments in the field of electrochemical biosensors with a focus on the sequence-specific detection of nucleic acids in real samples. After electrochemical hybridization sensors had been first described in 1993, it took nearly a decade until some of the many proposed protocols were indeed applied to real samples like blood or tissue. Electrochemical transduction schemes used either rely on electroactive moieties such as intercalators, groove binders, covalently attached labels, and products of enzyme markers or they are completely indicator free like impedance-based detection principles. Most detection schemes require a polymerase chain reaction amplification step to allow for sufficient selectivity and sensitivity. Today, several companies develop electrochemical microarrays able to detect dozens to many thousands of sequences in a single experiment.

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Electrochemical competitive hybridization assay for DNA detection using osmium tetroxide-labelled signalling strands

Cited by 19 publications

References 26 publications

An Electrochemical DNA Biosensor for the Detection of the Apa I Polymorphism in the Vitamin D Receptor Gene Using Meldola's Blue as a Hybridization Indicator

An Electrochemical DNA Biosensor for the Detection of the Apa I Polymorphism in the Vitamin D Receptor Gene Using Meldola's Blue as a Hybridization Indicator

Parallel Detection of Different DNA Sequences on One Gold Electrode

Sequence-specific electrochemical detection of nucleic acids in real samples

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