Electrochemical Characterization of Nanogap Interdigitated Electrode Arrays for Lab-on-a-Chip Applications

Matylitskaya, Volha; Urban, G.; Dincer, Can; Partel, Stefan

doi:10.1149/2.0701803jes

Cited by 18 publications

(13 citation statements)

References 52 publications

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“…Besides that, an additional electrochemical signal amplification via a so‐called redox cycling by using interdigitated electrode arrays (IDAs) can be combined with our microfluidic stop‐flow measurement protocol. In our previous published works, signal amplifications of more than 150 times are achieved, employing nanogap IDAs with 100 nm gap size . Combining this technique with our CRISPR‐based biosensor, the LOD of our system could be reduced to less than 100 f m .…”

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confidence: 89%

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CRISPR/Cas13a‐Powered Electrochemical Microfluidic Biosensor for Nucleic Acid Amplification‐Free miRNA Diagnostics

et al. 2019

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Noncoding small RNAs, such as microRNAs, are becoming the biomarkers of choice for multiple diseases in clinical diagnostics. A dysregulation of these microRNAs can be associated with many different diseases, such as cancer, dementia, and cardiovascular conditions. The key for effective treatment is an accurate initial diagnosis at an early stage, improving the patient's survival chances. In this work, the first clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a‐powered microfluidic, integrated electrochemical biosensor for the on‐site detection of microRNAs is introduced. Through this unique combination, the quantification of the potential tumor markers microRNA miR‐19b and miR‐20a is realized without any nucleic acid amplification. With a readout time of 9 min and an overall process time of less than 4 h, a limit of detection of 10 pm is achieved, using a measuring volume of less than 0.6 µL. Furthermore, the feasibility of the biosensor platform to detect miR‐19b in serum samples of children, suffering from brain cancer, is demonstrated. The validation of the obtained results with a standard quantitative real‐time polymerase chain reaction method shows the ability of the electrochemical CRISPR‐powered system to be a low‐cost, easily scalable, and target amplification‐free tool for nucleic acid based diagnostics.

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confidence: 89%

“…In our previous published works, signal amplifications of more than 150 times are achieved, employing nanogap IDAs with 100 nm gap size. [55][56][57] Combining this technique with our CRISPRbased biosensor, the LOD of our system could be reduced to less than 100 fm.…”

mentioning

confidence: 99%

CRISPR/Cas13a‐Powered Electrochemical Microfluidic Biosensor for Nucleic Acid Amplification‐Free miRNA Diagnostics

et al. 2019

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“…Besides an optimization of the immobilization strategy, an additional electrochemical signal amplification via a so-called redox cycling by interdigitated electrode arrays (IDAs) can be combined with our microfluidic stop-flow measurement protocol. In our previous published works, signal amplifications of more than 150-times is achieved, employing nanogap IDAs with 100 nm gap size [56][57][58] . With a combination of this technique and our CRISPR based biosensor, the LOD of our system could be reduced to less than 100 fM.…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas13a powered electrochemical microfluidic biosensor for nucleic acid amplification-free miRNA diagnostics

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Baaske

Chatelle

et al. 2019

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Non-coding small RNAs, such as microRNAs, are becoming the biomarkers of choice for multiple diseases in clinical diagnostics. A dysregulation of these microRNAs can be associated to many different diseases, such as cancer, dementia or cardiovascular conditions. The key for an effective treatment is an accurate initial diagnosis at an early stage, improving the patient's survival chances. Here, we introduce a CRISPR/Cas13a powered microfluidic, integrated electrochemical biosensor for the on-site detection of microRNAs. Through this unique combination, the quantification of the potential tumor markers microRNA miR-19b and miR-20a has been realized without any nucleic acid amplification. With a readout time of 9 minutes and an overall process time of less than 4 hours, a limit of detection of 10 pM was achieved, using a measuring volume of less than 0.6 µl. Furthermore, we demonstrate the feasibility of our versatile sensor platform to detect miR-19b in serum samples of children, suffering from brain cancer. The validation of our results with a standard qRT-PCR method shows the ability of our system to be a low-cost and target amplification-free tool for nucleic acid based diagnostics.MicroRNAs (miRNAs) are composed of 18-25 nucleotides in their mature form and play an essential role as regulators of gene expression in many biological processes by binding to specific messenger RNA targets and promoting their degradation or translational inhibition 1,2 . In recent years, the popularity of miRNAs in research has been constantly growing, as the presence or dysregulation of distinct miRNAs can indicate specific medical conditions. For instance, in cancer research the up or down regulation of miRNA expression levels has been linked to certain cancer types, including lung cancer or brain tumors. This offers new possibilities for the detection and monitoring of such diseases, which is why miRNAs will continue to come in the focus of clinical diagnostics 3-5 .

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“…Another commonly used material to fabricate IDEA is platinum (Pt). For instance, Matylitskaya et al [ 18 ] fabricated Pt nanogap IDEAs (nIDEAs) on silicon (Si) substrate for Lab-on-a-Chip applications (LoC). Ferrocenemethanol (FcMeOH) and p-aminophenol (pAP) were used as the redox couples to perform CV and CA electrochemical characterization of Pt nIDEAs.…”

Section: Micro and Nano Ideamentioning

confidence: 99%

“…The counter electrode must be of large surface area (since the current through the cell must be controlled by the reaction at the working electrode) with a stable and good conductor [ 16 ]. Carbon [ 17 ], platinum (Pt) [ 18 ], gold (Au) [ 19 ], and other materials [ 7 , 20 , 21 ] are the common materials used to fabricate the counter electrode. Reference electrodes are designed so that an equilibrium is set up with a known potential between a metal wire and the surrounding solution.…”

Section: Introductionmentioning

confidence: 99%

Micro and Nano Interdigitated Electrode Array (IDEA)-Based MEMS/NEMS as Electrochemical Transducers: A Review

et al. 2022

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Micro and nano interdigitated electrode array (µ/n-IDEA) configurations are prominent working electrodes in the fabrication of electrochemical sensors/biosensors, as their design benefits sensor achievement. This paper reviews µ/n-IDEA as working electrodes in four-electrode electrochemical sensors in terms of two-dimensional (2D) planar IDEA and three-dimensional (3D) IDEA configurations using carbon or metal as the starting materials. In this regard, the enhancement of IDEAs-based biosensors focuses on controlling the width and gap measurements between the adjacent fingers and increases the IDEA’s height. Several distinctive methods used to expand the surface area of 3D IDEAs, such as a unique 3D IDEA design, integration of mesh, microchannel, vertically aligned carbon nanotubes (VACNT), and nanoparticles, are demonstrated and discussed. More notably, the conventional four-electrode system, consisting of reference and counter electrodes will be compared to the highly novel two-electrode system that adopts IDEA’s shape. Compared to the 2D planar IDEA, the expansion of the surface area in 3D IDEAs demonstrated significant changes in the performance of electrochemical sensors. Furthermore, the challenges faced by current IDEAs-based electrochemical biosensors and their potential solutions for future directions are presented herein.

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Electrochemical Characterization of Nanogap Interdigitated Electrode Arrays for Lab-on-a-Chip Applications

Cited by 18 publications

References 52 publications

CRISPR/Cas13a‐Powered Electrochemical Microfluidic Biosensor for Nucleic Acid Amplification‐Free miRNA Diagnostics

CRISPR/Cas13a‐Powered Electrochemical Microfluidic Biosensor for Nucleic Acid Amplification‐Free miRNA Diagnostics

CRISPR/Cas13a powered electrochemical microfluidic biosensor for nucleic acid amplification-free miRNA diagnostics

Micro and Nano Interdigitated Electrode Array (IDEA)-Based MEMS/NEMS as Electrochemical Transducers: A Review

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