Electrochemical characterization of BSA/11-mercaptoundecanoic acid on Au electrode

Ignat, Teodora; Miu, M.; Kleps, I.; Brăgaru, Adina; Simion, Monica; Dănilă, M.

doi:10.1016/j.mseb.2009.11.021

Cited by 17 publications

(14 citation statements)

References 35 publications

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“…All these features make PmB a promising candidate ligand for LPS in a bioreceptor aimed at detecting endotoxin (Chang, 1997). To ensure the correct immobilization of the ligand and to avoid nonspecific adsorption of endotoxin onto the surface of the sensors, PmB was covalently linked to self-assembled monolayers (SAMs) previously formed on the gold electrodes (Ignat et al, 2010). SAMs are organized structures of organic molecules that allow the efficient and simple immobilization of different compounds used for biological detection (Pillay et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Novel fully-integrated biosensor for endotoxin detection via polymyxin B immobilization onto gold electrodes

Zuzuarregui

Arana

Pérez-Lorenzo

et al. 2013

J. Sens. Sens. Syst.

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Abstract. In this paper an electrochemical endotoxin biosensor consisting of an immobilized lipopolysaccharide (LPS) ligand, polymyxin B (PmB), is presented. Several parameters involved both in the device fabrication and in the detection process were analyzed to optimize the ligand immobilization and the interaction between PmB and LPS, aiming at increasing the sensitivity of the sensor. Different electrochemical pre-treatment procedures as well as the functionalization methods were studied and evaluated. The use of a SAM (self-assembled monolayer) to immobilize PmB and the quantification of the interactions via cyclic voltammetry allowed the development of a robust and simple device for in situ detection of LPS. Thus, the biosensor proposed in this work intends an approach to the demanding needs of the market for an integrated, portable and simple instrument for endotoxin detection.

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Section: Introductionmentioning

confidence: 99%

Novel fully-integrated biosensor for endotoxin detection via polymyxin B immobilization onto gold electrodes

Zuzuarregui

Arana

Pérez-Lorenzo

et al. 2013

J. Sens. Sens. Syst.

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“…By this method, proteins can be chemically bound with controlled order and density on the gold surface. Because SAs are biological macromolecules, their native structures should be carefully preserved during immobilization to maintain their functionality . Researchers have used SAM techniques to successfully fabricate various QCM biosensors, which exhibited excellent response and bioactivity .…”

Section: Introductionmentioning

confidence: 99%

“…Because SAs are biological macromolecules, their native structures should be carefully preserved during immobilization to maintain their functionality. [19][20][21][22] Researchers have used SAM techniques to successfully fabricate various QCM biosensors, which exhibited excellent response and bioactivity. [23][24][25][26] Herein, SAM was selected as the immobilization method to anchor proteins.…”

Section: Introductionmentioning

confidence: 99%

A New Biosensor for Chiral Recognition Using Goat and Rabbit Serum Albumin Self‐Assembled Quartz Crystal Microbalance

Chen

Zhang

et al. 2012

Chirality

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Quartz crystal microbalance (QCM) biosensor was used for the chiral recognition of five pairs of enantiomers by using goat serum albumin (GSA) and rabbit serum albumin (RbSA) as chiral selectors. Serum albumin (SA) was immobilized on the QCM through the self-assembled monolayer technique, and the surface concentration of GSA and RbSA were 8.8 × 10(-12) mol cm(-2) and 1.2 × 10(-11) mol cm(-2) , respectively. The QCM biosensors showed excellent sensitivity and selectivity. Meanwhile, the chiral recognition of SA sensors was quite species dependent. There were differences between GSA and RbSA sensors in the ability and the preference of chiral recognition. To R,S-1,2,3,4-tetrahydro-1-naphthylamine (R,S-1-TNA), R,S-1-(4-methoxyphenyl)ethylamine (R,S-4-MPEA), and R,S-1-(3-methoxyphenyl)ethylamine (R,S-3-MPEA), the preference of the stereoselective SA-drug binding of the two kinds of SA sensors were consistent. However, to R,S-2-octanol (R, S-2-OT) and R,S-methyl lactate (R,S-MEL), the two kinds of SA sensors had opposite chiral recognition preference. Moreover, the interactions of SA and the five pairs of enantiomers have been further investigated through ultraviolet (UV) and fluorescent (FL) spectra. The UV/FL results were in accordance with the consequence of QCM.

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“…BSA is a rigid, neutral, stable, moderately non-reactive protein [2,3]. It adsorbs to any type of surface and has high stability [4].…”

Section: Methodsmentioning

confidence: 99%

Influence of the Geometry on the LTCC Integrated Electrochemical Cells Performance

2018

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Miniaturized and integrated analytical devices, including chemical sensors, are at the forefront of modern analytical chemistry. The construction of novel analytical tools takes advantage of contemporary micro- and nanotechnologies, as well as materials science and technology. The goal of this study was investigate electron transfer resistance in model solution and protein adsorption using integrated electrochemical cell with different geometry.

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Electrochemical characterization of BSA/11-mercaptoundecanoic acid on Au electrode

Cited by 17 publications

References 35 publications

Novel fully-integrated biosensor for endotoxin detection via polymyxin B immobilization onto gold electrodes

Novel fully-integrated biosensor for endotoxin detection via polymyxin B immobilization onto gold electrodes

A New Biosensor for Chiral Recognition Using Goat and Rabbit Serum Albumin Self‐Assembled Quartz Crystal Microbalance

Influence of the Geometry on the LTCC Integrated Electrochemical Cells Performance

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