Electrochemical biosensors based on nucleic acid aptamers

Villalonga, Anabel; Pérez-Calabuig, Ana M.; Villalonga, Reynaldo

doi:10.1007/s00216-019-02226-x

Cited by 138 publications

(81 citation statements)

References 82 publications

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“…Regardless of which system is used the path ahead is clear. A rapid, portable, POC detection test is needed and could be used not only in hospitals, but also, the food industry, agriculture, environmental monitoring and homeland security and defense [60,135].…”

Section: Discussionmentioning

confidence: 99%

Novel Detection of Nasty Bugs, Prevention Is Better than Cure

Strom

Crowley

Shigdar

2020

IJMS

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Hospital-acquired infections (HAIs) are a growing concern around the world. They contribute to increasing mortality and morbidity rates and are an economic threat. All hospital patients have the potential to contract an HAI, but those with weakened or inferior immune systems are at highest risk. Most hospital patients will contract at least one HAI, but many will contract multiple ones. Bacteria are the most common cause of HAIs and contribute to 80–90% of all HAIs, with Staphylococcus aureus, Clostridium difficile, Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae accounting for the majority. Each of these bacteria are highly resistant to antibiotics and can produce a protective film, known as a biofilm, to further prevent their eradication. It has been shown that by detecting and eradicating bacteria in the environment, infection rates can be reduced. The current methods for detecting bacteria are time consuming, non-specific, and prone to false negatives or false positives. Aptamer-based biosensors have demonstrated specific, time-efficient and simple detection, highlighting the likelihood that they could be used in a similar way to detect HAI-causing bacteria.

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Section: Discussionmentioning

confidence: 99%

Novel Detection of Nasty Bugs, Prevention Is Better than Cure

Strom

Crowley

Shigdar

2020

IJMS

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“…The aptamer is composed of several nucleic acid or peptide sequences that can bind with high affinity to specific targets like proteins, small molecules, chemicals, and nucleic acids 33 . These non-canonical nucleic acids and peptides have been applied to therapeutic agents in pharmaceutical science 34 and detection elements for various biosensors 35 .…”

Section: Introductionmentioning

confidence: 99%

Recent Development of Aptasensor for Influenza Virus Detection

Kim

Noh

et al. 2020

BioChip J

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In nowadays, we have entered the new era of pandemics and the significance of virus detection deeply impacts human society. Viruses with genetic mutations are reported nearly every year, and people have prepared tools to detect the virus and vaccines to ensure proper treatments. Influenza virus (IV) is one of the most harmful viruses reporting various mutations, sub-types, and rapid infection speed for humans and animals including swine and poultry. Moreover, IV infection presents several harmful symptoms including cough, fever, diarrhea, chills, even causing death. To reduce the IV-induced harm, its proper and rapid detection is highly required. Conventional techniques were used against various IV sub-types including H1N1, H3N2, and H5N1. However, some of the techniques are time-consuming, expensive, or labor-intensive for detecting IV. Recently, the nucleic acid-based aptamer has gained attention as a novel bioprobe for constructing a biosensor. In this review, the authors discuss the recent progress in aptasensors for detecting IV in terms of an electrochemical and an optical biosensor.

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“…They are readily accessible but can be manufactured straightforwardly (22,23). Besides their high specificity and dynamic binding towards corresponding targets, aptamers can be effortlessly modified through the incorporation of a wide range of functional substitutes, making them suitable for a variety of applications (20,24). Generally, biosensorbased determination methods with their substantial selectivity and cost-effectiveness can offer reliable alternatives to the conventional analytical assays (25).…”

Section: Introductionmentioning

confidence: 99%

A Sensitive Aptamer-Based Biosensor for Electrochemical Quantification of PSA as a Specific Diagnostic Marker of Prostate Cancer

et al. 2020

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Purpose: The current project aimed to design a simple, highly sensitive, and economical label-free electrochemical aptasensor for determination of prostate-specific antigen (PSA), as the gold standard biomarker for prostate cancer diagnosis. The aptasensor was set up using a screen-printed carbon electrode (SPCE) modified by gold nanoparticles (Au NPs) conjugated to thiolated aptamers. Methods: Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were implemented for electrochemical (EC) characterization of the aptasensor. The determination of PSA was also performed through differential pulse voltammetry (DPV) in [Fe (CN) 6]3-/4- electrolyte solution. Results: The present aptasensor was shown an outstanding linear response in the concentration range of 1 pg/mL - 200 ng/mL with a remarkably lower limit of detection of 0.077 pg/mL. The optimum concentration for PSA separation and the optimum incubation time for antigen-aptamer binding were determined by observing and electing the highest electrochemical responses in a specified time or concentration. Conclusion: According to the results of the specificity tests, the designed aptasensor did not show any significant interactions with other analytes in real samples. Clinical functionality of the aptasensor was appraised in serum samples of healthy individuals and patients examining the PSA level through the fabricated aptasensor and the reference methods. Both methods are comparable in sensitivity. The present fabricated PSA aptasensor with substantial characteristics of ultra-sensitivity and cost-effectiveness can be conventionally built and used for the routine check-up of the men for prostate problems.

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Electrochemical biosensors based on nucleic acid aptamers

Cited by 138 publications

References 82 publications

Novel Detection of Nasty Bugs, Prevention Is Better than Cure

Novel Detection of Nasty Bugs, Prevention Is Better than Cure

Recent Development of Aptasensor for Influenza Virus Detection

A Sensitive Aptamer-Based Biosensor for Electrochemical Quantification of PSA as a Specific Diagnostic Marker of Prostate Cancer

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