Electrochemical biosensors and nanobiosensors

Hammond, Jules L.; Formisano, Nello; Estrela, Pedro; Carrara, Sandro; Tkac, Jan

doi:10.1042/ebc20150008

Cited by 294 publications

(140 citation statements)

References 56 publications

(58 reference statements)

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“…This configuration, including a working, counter and reference electrode (Working Electrode (WE), Counter Electrodes (CE), and Reference Electrode (RE)), is most common for typical electrochemical applications, especially when the potential across the electrochemical interface need to be measured accurately, in order to ensure stability of the cell [8].…”

mentioning

confidence: 99%

Wireless Point-of-Care Platform With Screen-Printed Sensors for Biomarkers Detection

Tonello

Abate

Borghetti

et al. 2017

IEEE Trans. Instrum. Meas.

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Abstract-Measurement systems for early and reliable detection of degenerative diseases, such as Alzheimer's disease (AD), are extremely important in clinical diagnosis. Among these, biochemical assays represent a commonly used method to distinguish patients from healthy population thanks to the sensitive recognition of specific biomarkers in biological fluids. In order to overcome actual limitations of these techniques in term of cost, standardization, and sensitivity, this study aimed to realize a low-cost highly sensitive portable point-of-care (PoC) testing system based on screen-printed electrochemical sensors. The development of the platform specifically included both the design of the sensing probe and the electronic circuit devoted to condition and acquires the transduced electric signal. The designed circuit was implemented in a printed circuit board and interfaced to a wireless system based on bluetooth data transmission in order to improve the portability of the proposed solution. Preliminary results were obtained by using controlled concentrations of electrolytic solutions and calibrating the sensors for antibodies and for a well-known protein (i.e., interleukin 8) quantified by anodic stripping voltammetry (ASV). Findings from ASV measurements showed a sensitivity of 38 µA/(ng/ml) with a tested range from 1.25 to 20 ng/ml, with a limit of detection of 2 ng/ml. Further investigation will include the validation of this PoC device by testing the concentration of a specific p53 protein isoform, which was recently identified to early correlate to AD development.

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mentioning

confidence: 99%

Wireless Point-of-Care Platform With Screen-Printed Sensors for Biomarkers Detection

Tonello

Abate

Borghetti

et al. 2017

IEEE Trans. Instrum. Meas.

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“…Bu üstün özelliklerden dolayı, CNT'lere dayanan biyosensörlerin geleneksel karbon elektrotlara dayananlara kıyasla daha yüksek duyarlıklara ve daha düşük gözlenebilme sınırlarına sahip olduğu belirlenmiştir [20]. CNT'ler çözücülerin pek çoğunda çözünmediğinden, bu bileşiklerin N,Ndimetilformamit (DMF) [21], Nafyon [22], kitosan [23], jelatin [24] gibi maddelerde dağıtılıp elde edilen karışımın uygun katı elektrot yüzeylerine damlatılması yolu ile modifiye elektrot hazırlanması en sık kullanılan yöntemdir.…”

Section: şEkil 1 Ksantin Biyosensörlerin Hazırlanışıunclassified

“…Çalışma sonucunda en uygun çalışma pH'sı 7,0 olarak belirlendi ve bu değer tüm biyosensörler ile yapılan çalışmalarda kullanıldı. Literatür incelendiğinde optimum olarak bulunan bu pH'ın daha önce rapor edilmiş pek çok ksantin biyosensöründe kullanılan pH değeri ile uyumlu olduğu bulunmuştur [20,[30][31][32][33][34].…”

Section: Elektrot Bileşiminin Optimizasyonuunclassified

“…Bu biyosensörün doğrusal çalışma aralığı 9,9×10 -7 -9,4×10 -4 M, gözlenebilme sınırı ise 8,7×10 -7 M olarak bulundu. Literatürde rapor edilen MWCNT'lere dayalı ksantin biyosensörleri incelendiğinde çalışmamızda NH2-MWCNT ile hazırlanan biyosensör ile elde edilen çalışma aralığı Luteolin/MWCNT modifiye GCE'ye dayalı biyosensörden (1,0×10 -6 -7,0×10 -5 M) [34], çinko oksit nanopartikül/c-MWCNT/polianilin modifiye Pt elektroda dayalı biyosensörden (1,0×10 -4 -1,0×10 -1 M) [30], polimerik medyatör/MWCNT'e dayalı biyosensörden (2,0×10 -6 -2,8×10 -5 M, 2,8×10 -5 -4,6×10 -5 M, 4,6×10 -5 -8,6×10 -5 M) [20] ve kobalt oksit nanopartikül/MWCNT modifiye GCE'ye dayalı biyosensörden (2,0×10 -7 -1,6×10 -5 M) [22] daha geniş bulundu. Biyosensörün gözlenebilme sınırının da literatürdeki bazı ksantin biyosensörlerinden daha düşük [35,36] bazıları ile ise kıyaslanabilir olduğu görüldü [34,37].…”

Section: Biyosensörlerin Analitik Cevabıunclassified

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Ksantin Biyosensörlerin Performansına Çeşitli Çok Duvarlı Karbon Nanotüplerin Etkisi

Kaçar

Dalkıran

Erden

et al. 2018

SDÜ Fen Bil Enst Der

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Özet: Bu çalışmada, fonksiyonel grup içermeyen (MWCNT) ve karboksil (COOH-MWCNT), hidroksil (OH-MWCNT) ve amino (NH2-MWCNT) grubu içeren çok duvarlı karbon nanotüplerin amperometrik ksantin biyosensörünün cevabına etkileri incelendi. Bu amaçla, MWCNT'ler ve ksantin oksidaz (XO) enzimi jelatin çözeltisi içinde dağıtıldı ve bu karışımlardan belirli miktarların camsı karbon elektrot yüzeyine damlatılması ile ksantin biyosensörleri hazırlandı. Bu biyosensörlerin optimum pH, enzim miktarı gibi çalışma koşulları belirlendi ve bu koşullarda biyosensörlerin performans faktörleri incelendi. NH2-MWCNT ile hazırlanan biyosensörün en yüksek duyarlık ile ksantine cevap verdiği gözlendi. Bu biyosensör ksantine <15 s gibi kısa bir cevap süresi ile 9,9×10 -7 M -9,4×10 -4 M aralığında doğrusal cevap gösterdi ve gözlenebilme sınırı 8,7×10 -7 M, duyarlığı 23,36 µA mM -1 olarak belirlendi. The Effect of Various Multiwalled Carbon Nanotubes on the Performance of Xanthine BiosensorsKeywords Biosensor, Amperometry, Multiwalled carbon nanotube, Xanthine, Xanthine oxidase Abstract:In this study effects of multiwalled carbon nanotubes (MWCNT), and multiwalled carbon nanotubes with carboxyl (COOH-MWCNT), hydroxyl (OH-MWCNT) and amino (NH2-MWCNT) functional groups on the response of amperometric xanthine biosensors were investigated. For this purpose, MWCNT and xanthine oxidase (XO) enzyme were dispersed in gelatin solution and aliquots of this solution was drop casted onto the surface of glassy carbon electrode in order to prepare the xanthine biosensors. Working conditions such as optimum pH and enzyme amount of these biosensors were investigated and analytical performances of the biosensors were determined under this optimum conditions. The biosensor prepared with NH2-MWCNT exhibited a xanthine response with the highest sensitivity. The response time, linear working range, detection limit and sensitivity of this biosensor was <15 s; 9.9×10 -7 M -9.4×10 -4 M; 8.7×10 -7 M; 23.36 µA mM -1 respectively.

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“…IDE is an electrochemical biosensor having promising features such as low-cost, portable and sensitive, makes a wide range of applications, in particular with environmental monitoring and medical diagnosis [11,12]. Enhancing the electrical property on the sensing surface improves the detection of biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Nanodetection of Head and Neck Cancer on Titanium Oxide Sensing Surface

Wang

Guo

et al. 2020

Nanoscale Res Lett

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Head and neck cancer is a heterogeneous disease, originating in the squamous cells lining the larynx (voice box), mouth, pharynx (throat), nasal cavity and salivary glands. Head and neck cancer diagnosis at the later stage is greatly influencing the survival rate of the patient. It makes a mandatory situation to identify this cancer at the earlier stages of development with a suitable biomarker. Squamous cell carcinoma antigen (SCC-Ag) is a circulating serum tumour biomarker, and the elevated level has been found in the head and neck cancer patients and highly correlated with the tumour volume. The present research was carried out to detect and quantify the level of SCC-Ag on titanium oxide (TiO 2 )-modified interdigitated electrode sensor (IDE) by SCC-Ag antibody. The detection of SCC-Ag was found at the level of 100 fM, while it was improved to 10 fM when the antibody was conjugated with gold nanostar, representing a 10-fold improvement. Interestingly, this enhancement in sensitivity is 1000-folds higher than other substrates. Moreover, the specificity analysis was carried out using two different control proteins and noticed that the antibody only recognised SCC-Ag, indicating the specific detection on IDE-TiO 2 sensing surface.

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Electrochemical biosensors and nanobiosensors

Cited by 294 publications

References 56 publications

Wireless Point-of-Care Platform With Screen-Printed Sensors for Biomarkers Detection

Wireless Point-of-Care Platform With Screen-Printed Sensors for Biomarkers Detection

Ksantin Biyosensörlerin Performansına Çeşitli Çok Duvarlı Karbon Nanotüplerin Etkisi

Nanodetection of Head and Neck Cancer on Titanium Oxide Sensing Surface

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