Electrochemical Biosensor for the Determination of Amlodipine Besylate Based on Gelatin–Polyaniline Iron Oxide Biocomposite Film

Djaalab, Elbahi; Samar, Mohamed El Hadi; Zougar, Saida; Kherrat, R.

doi:10.3390/catal8060233

Cited by 19 publications

(9 citation statements)

References 42 publications

(37 reference statements)

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“…AML alone has been estimated by spectrophotometric, [9, 10] fluorometric, [11] reverse-phase high-performance liquid chromatography (RP-HPLC), [12–14] and liquid chromatography–tandem mass spectrometry (LCMS/MS) methods [15]. AML, along with other antihypertensive drugs, were determined by spectrophotometric [16], high-performance thin-layer chromatography (HPTLC), [17] and electroanalytical methods [18–23]. Besides, few high-performance liquid chromatography (HPLC) procedures have been described for the concurrent estimation of AML with statins.…”

Section: Introductionmentioning

confidence: 99%

Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation

et al. 2019

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Recently, the United States Food and Drug Administration approved a new oral dosage preparation of amlodipine besylate (AML) and celecoxib (CEL) for the management of hypertension and osteoarthritis. However, no simultaneous estimation procedures for these two analytes have been described. Hence, two simple, accurate, and precise ultraviolet spectroscopic procedures that manipulated the ratio spectra were established for concurrent quantification of AML and CEL using ethanol as a solvent. The first method involves determining the peak-to-trough amplitude difference of the ratio spectra of AML and CEL. The second method involves determining the peak amplitude of the ratio first derivative (Δλ 4 nm) spectra of AML and CEL at 334.2 nm and 254.2 nm, correspondingly. Both methods showed linearity in the range of 1–6 μg mL-1 for AML and 5–40 μg mL-1 for CEL with an excellent correlation coefficient (<0.999). The proposed procedures were validated by following the International Conference on Harmonization guidelines for accuracy, precision, selectivity, recovery, and stability studies. It is evident from the low %RSD and %RE that both analytical procedures were found to be accurate and precise, respectively. The percent recovery of AML and CEL from the formulation was found to be 99.79% and 99.34% using the ratio-difference method and 100.13% and 99.70% using the ratio first-derivative method, with a low percent relative standard deviation. Further, the proposed techniques permit concurrent quantification of AML and CEL in different concentration ratios without interference from each other; hence, these techniques can be adopted for regular quality-control studies.

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Section: Introductionmentioning

confidence: 99%

Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation

et al. 2019

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“…By using the method of crosslinked gelatin immobilization, many enzyme-based biosensors were developed for medical diagnosis, such as Pt/zein/gelatin-GOx biosensors ( Figure 1 a) [ 33 ], three-electrode array biosensors ( Figure 1 b) [ 34 ], Pt-PANI@Fe 2 O 3 -GA biosensors ( Figure 1 c) [ 35 ], gelatin/GOx-Pt glucose biosensors ( Figure 1 d) [ 36 ], and AsOx/GB-ZnO/ITO ascorbic acid biosensors ( Figure 1 e) [ 37 ].…”

Section: Gelatin As a Matrix For Immobilized Biorecognition Materialsmentioning

confidence: 99%

Applications of Gelatin in Biosensors: Recent Trends and Progress

Guan

Huang

2022

Biosensors

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Gelatin is a natural protein from animal tissue with excellent biocompatibility, biodegradability, biosafety, low cost, and sol–gel property. By taking advantage of these properties, gelatin is considered to be an ideal component for the fabrication of biosensors. In recent years, biosensors with gelatin have been widely used for detecting various analytes, such as glucose, hydrogen peroxide, urea, amino acids, and pesticides, in the fields of medical diagnosis, food testing, and environmental monitoring. This perspective is an overview of the most recent trends and progress in the development of gelatin-based biosensors, which are classified by the function of gelatin as a matrix for immobilized biorecognition materials or as a biorecognition material for detecting target analytes.

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“…For example, a biosensor based on lipase was developed for amlodipine besylate (AMD) drug using a mixture of polyaniline, iron oxide and gelatin (Figure 4). After preparation of the sensitive material (step 1), the enzyme was entrapped in the biocomposite matrix film with the aid of a glutaraldehyde cross-linking reagent (step 2) to establish the immobilization of the lipase (step 3) [109]. Cyclic voltammetry (A) and impedometry (B) were then used for detection experiments which proved that such material was a good candidate for the construction of a sensitive biosensor for AMD analysis (Figure 4b).…”

Section: Conducting Polymer-based Enzyme Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors Based on Conducting Polymers: A Review

Lakard

2020

Applied Sciences

112

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Conducting polymers are an important class of functional materials that has been widely applied to fabricate electrochemical biosensors, because of their interesting and tunable chemical, electrical, and structural properties. Conducting polymers can also be designed through chemical grafting of functional groups, nanostructured, or associated with other functional materials such as nanoparticles to provide tremendous improvements in sensitivity, selectivity, stability and reproducibility of the biosensor’s response to a variety of bioanalytes. Such biosensors are expected to play a growing and significant role in delivering the diagnostic information and therapy monitoring since they have advantages including their low cost and low detection limit. Therefore, this article starts with the description of electroanalytical methods (potentiometry, amperometry, conductometry, voltammetry, impedometry) used in electrochemical biosensors, and continues with a review of the recent advances in the application of conducting polymers in the recognition of bioanalytes leading to the development of enzyme based biosensors, immunosensors, DNA biosensors, and whole-cell biosensors.

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Electrochemical Biosensor for the Determination of Amlodipine Besylate Based on Gelatin–Polyaniline Iron Oxide Biocomposite Film

Cited by 19 publications

References 42 publications

Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation

Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation

Applications of Gelatin in Biosensors: Recent Trends and Progress

Electrochemical Biosensors Based on Conducting Polymers: A Review

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