Electrochemical biosensor for investigation of anticancer drugs interactions (doxorubicin and ellipticine) with DNA

Abstract

The mechanisms of action of chemotherapeutics are different, but very often interfere with processes of cell division and can bind onto DNA. The aim of this work was to investigate interactions of antitumour drugs (ellipticine and doxorubicin) with DNA. For these purposes our experiments the screen-printed nanotube carbon electrodes were used. DNA gives oxidation signals for all bases (adenine, cytosine, guanine and thymine). We introduced various ellipticine, doxorubicin and ethidium bromide concentrations to DNA on carbon electrodes fabricated by us and measured the changes in the height of nucleic acid bases signals. In the voltammograms measured the changes associated with the interactions of the target compounds with DNA were evident. The height of DNA signals decreased with increasing ellipticine, doxorubicin and ethidium bromide concentrations. The decrease can be related to changes of DNA structure caused by the intercalation.

Electrochemical DNA Sensors with Layered Polyaniline—DNA Coating for Detection of Specific DNA Interactions

“…DNA immobilized onto reduced graphene oxide decorated with Au nanoparticles has been employed for quantification of interaction with doxorubicin by DPV technique using oxidation peak current promoted by the drug binding (limit of detection (LOD) 8 μg/mL) [10]. Various DNA intercalators have been detected with carbon nanotube printed electrode in the presence of single- and double-stranded DNA molecules based on nucleotide oxidation currents [11]. Among others, doxorubicin was determined in micromolar range of its concentrations.…”

The Study of Naphthoquinones and Their Complexes with DNA by Using Raman Spectroscopy and Surface Enhanced Raman Spectroscopy: New Insight into Interactions of DNA with Plant Secondary Metabolites

“…Their cytotoxic effect is based on their interactions with DNA, especially on the possibility of forming various types of covalent adducts with DNA. The most important compounds, whose effect is based on DNA interaction, are anthracyclines, cytotoxic antibiotics, and platinum-based cytostatics [ 18 – 21 ].…”