Electrochemical biosensor evaluation of the interaction between DNA and metallo-drugs

Abstract

Electrochemical techniques were used to study the interaction between a panel of antiproliferative metallo-drugs and double-stranded DNA immobilized on screen-printed electrodes as a model of the analogous interaction occurring in solution. The propensity of a given metal drug to interact with DNA was measured as a function of the decrease of guanine oxidation signal, which was detected by square wave voltammetry. Estimates of variations in experimental parameters, such as the concentration of complexes, time following dissolution (ageing time) and the presence of chloride, are provided.

Voltammetric and spectroscopic studies on the interaction of tilmicosin with bovine serum albumin at different pHs

“…Moreover, the determination and monitoring of drug-protein adducts have important clinical, pharmacological and toxicological implications [11]. UV spectrometry [12][13][14][15] and electrochemical methods [6,[16][17][18][19] have been generally used in the studies on the interaction of drug-protein.…”

The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

“…This approach has been successfully employed for the study of the interaction between a series of antitumor metallo-drugs and DNA saline solution [6][7][8][9]. A complete, albeit qualitative, description concerning (i) the reactivity of the metal complex, (ii) the possible mass effect of anions acting as leaving ligands (i.e., chlorides), (iii) the intrinsic affinity of the generated electrophilic agent to DNA, and (iv) the strength of perturbation caused in the DNA chain by such metallo-drugs can be obtained using this technique.…”

“…The DNA-biosensor protocol, utilized in the previous experiments [8], consisted of four steps: (i) electrochemical conditioning of the SPE surface, (ii) electrochemical deposition of ds-DNA onto SPE, (iii) interaction of the drug with the immobilized DNA for a brief period of time, and (iv) interrogation of the electrode surface by dipping the electrode in a suitable buffer, and performing a SWV scan. In the present study, a new experimental protocol was developed in order to increase the sensitivity.…”

Synthesis, spectroscopic, thermal, electrical conductivity and antimicrobial interpretations of new VO(II), Zn(II), Pd(II), Au(III) and Pt(IV) complexes with hypertensive atenolol drug

“…The values of X M as calculated from equation (2) are corrected for the diamagnetism of the ligands using Pascal's constants, and then applied in Curie's equation (3).…”