Electrochemical biosensor based on base-stacking-dependent DNA hybridization assay for protein detection

Li, Hao; Zhang, Wei; Zhou, Hong-Hao

doi:10.1016/j.ab.2013.12.019

Cited by 10 publications

(2 citation statements)

References 37 publications

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“…The working area was incubated with 0.1 mM of MCH at room temperature for 5 minutes then washed thoroughly. Here, using the MCH enhances the capture probe alignment, displaces any weakly adsorbed and nonspecific capture probes bound to the working area (Steel et al, 1998;Steel et al, 2000) and reduces as well the steric and electrostatic hindrances among the packed capture probes (Herne and Tarlov, 1997;Si et al, 2014;Li et al, 2010;Li et al, 2014;Jiang et al, 2019).…”

Section: Immobilization Stepsmentioning

confidence: 99%

Developing a nano-biosensor for early detection of pancreatic cancer

Rawashdeh

Al-Fandi

Makableh

et al. 2020

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Purpose The purpose of this paper is to report on the development of a simple electrochemical nanobiosensor for early detection of pancreatic cancer. The nanobiosensor uses the newly emerged stable micro ribonucleic acid (miR-21) as a cancer-associated biomarker for diagnosis, prognosis or therapy response. Design/methodology/approach The biosensing practice consists of two main steps: capturing probe immobilization on a working electrode modified with multi-walled carbon nanotubes and gold nanoparticles (MWCNTs-AuNPs) and then sensing the miR-21 interaction electrochemically. Two electrochemical techniques, atomic force microscopy and Fourier-transform infrared spectroscopy, were applied for characterizations. Findings The nanobiosensor sensitivity exhibited satisfying results to miR-21 and demonstrated a wide dynamic range with a detection limit of just about 3.68 femtomolar using the source measure unit (SMU). Originality/value Researchers commonly use potentiostats to perform the differential pulse voltammetry (DPV) measurements for the electrochemical biosensing applications. In this study, the SMU was used to perform the DPV to detect the biomarker miR-21 using the MWCNTs-AuNPs screen-printed electrode as the electrochemical system.

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Section: Immobilization Stepsmentioning

confidence: 99%

Developing a nano-biosensor for early detection of pancreatic cancer

Rawashdeh

Al-Fandi

Makableh

et al. 2020

View full text Add to dashboard Cite

show abstract

“…This highly sensitive detection method may depend on the following factors: on the one hand, ILs provided a large number of active sites and were employed as substrate for the immobilization of considerable amount of HRP-labeled anti-S. pullorum. On the other hand, the AuNPs and HRP amplified the electrochemical signal, which could further enhance the sensitivity of the immunosensor (Kang et al, 2013;Li et al, 2014).…”

Section: Calibration Curve Of the Immunosensormentioning

confidence: 99%