Electrochemical bioplatforms for the simultaneous determination of interleukin (IL)-8 mRNA and IL-8 protein oral cancer biomarkers in raw saliva

Torrente‐Rodríguez, Rebeca M.; Campuzano, Susana; Montiel, Víctor Ruiz‐Valdepeñas; Gamella, María; Pingarrón, José M.

doi:10.1016/j.bios.2015.10.016

Cited by 90 publications

(48 citation statements)

References 22 publications

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“…5c which shows that this saliva-based assay exhibits a linear response from 1 pg/mL to 100 ng/mL (R 2 = 0.9655). Similar to PBS, this Pf HRP2 assay exhibits a very low limit of detection of 2.2 pg/mL in unadulterated saliva, which is 30× lower than previously reported electrochemical measurements of protein biomarker in raw saliva (Torrente-Rodríguez et al 2016). Furthermore, the detection range of this sensor is within the clinical concentrations found in patients with P. falciparum infection (Wilson et al 2008; Fung et al 2012).…”

Section: Resultsmentioning

confidence: 64%

Enzyme-free electrochemical immunosensor based on methylene blue and the electro-oxidation of hydrazine on Pt nanoparticles

Dutta

Nagarajan

Lapidus

et al. 2017

Biosensors and Bioelectronics

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Enzyme-free electrochemical sensors enable rapid, high sensitivity measurements without the limitations associated with enzyme reporters. However, the performance of non-enzymatic electrochemical sensors tends to suffer from slow electrode kinetics and poor signal stability. We report a new enzyme-free electrochemical immunosensor based on a unique competitive detection scheme using methylene blue (MB), hydrazine and platinum nanoparticles (Pt NPs). This scheme is coupled with a robust immunosandwich format employing a MB-labelled detection antibody as a non-enzymatic reporter. In the presence of the target antigen, surface-immobilized MB consumes interfacial hydrazine thereby diminishing the electro-oxidation of hydrazine on Pt NPs. Thus, the concentration of the antigen is directly proportional to the reduction in the electrochemical signal. For proof-of-concept, this sensor was used to detect Plasmodium falciparum histidine-rich protein 2 (PfHRP2), an important malaria biomarker, in unadulterated human saliva samples. Chronocoulometric measurements showed that this platform exhibits pM-range sensitivity, high specificity and good reproducibility, making it well suited for many biosensing applications including noninvasive diagnostic testing.

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Section: Resultsmentioning

confidence: 64%

Enzyme-free electrochemical immunosensor based on methylene blue and the electro-oxidation of hydrazine on Pt nanoparticles

Dutta

Nagarajan

Lapidus

et al. 2017

Biosensors and Bioelectronics

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“…In the recent years, many examples have been proposed for the electrochemical detection of DNA and RNA sequences using disposable electrodes [64][65][66][67][68][69][70][71]. Among these, many are those based on different signal amplification procedures that allow to achieve very low detection limits [72][73][74][75][76][77][78][79][80].…”

Section: Dna-based Electrochemical Disposable Sensorsmentioning

confidence: 99%

“…In the period considered in this review several examples have been proposed to develop electrochemical disposable sensors based on the use of DNA aptamers or DNA sequences for the detection of aptamers' targets, proteins, transcription-factors and anti-DNA antibodies [65,[90][91][92][93][94][95].…”

Section: Dna-based Electrochemical Disposable Sensorsmentioning

confidence: 99%

Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis

Arduini

Micheli

Moscone

et al. 2016

TrAC Trends in Analytical Chemistry

317

166

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A B S T R A C TThis review addresses recent advances in the development of screen-printed electrode based biosensors modified with different nanomaterials such as carbon nanotubes, graphene, metallic nanoparticles as gold, silver and magnetic nanoparticles, and mediator nanoparticles (Prussian Blue, Cobalt Phthalocyanine, etc.), coupled with biological recognition elements such as enzymes, antibodies, DNA and aptamers to obtain probes with improved analytical features. Examples of clinical applications are illustrated, together with examples of paper-based electrochemical devices, of multiple detections using arrays of screen printed electrodes, and of the most recent developments in the field of wearable biosensors. Also the use of smartphones as final detectors is briefly depicted.

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“…These disposable biosensors provided a LOD of 2.9 nM for the synthetic target without any target or signal amplification process as well as complete discrimination towards a single base mismatch in untreated and undiluted spiked human serum and saliva samples. The same group developed a MB-based genosensing approach for the determination of mRNA associated to interleukin-8 (IL-8) mRNA [25]. The method was based on the hybridization of the target biotinylated oligonucleotide onto Strep-MBs modified with a specific biotinylated hairpin probe, labeling with a Strep-HRP polymer and amperometric detection at SPCEs using the H 2 O 2 /hydroquinone (HQ) system.…”

Section: Electrochemical Genosensing Of Circulating Biomarkersmentioning

confidence: 99%

Electrochemical Genosensing of Circulating Biomarkers

Campuzano

Yáñez‐Sedeño

Pingarrón

2017

Sensors

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Management and prognosis of diseases requires the measurement in non- or minimally invasively collected samples of specific circulating biomarkers, consisting of any measurable or observable factors in patients that indicate normal or disease-related biological processes or responses to therapy. Therefore, on-site, fast and accurate determination of these low abundance circulating biomarkers in scarcely treated body fluids is of great interest for health monitoring and biological applications. In this field, electrochemical DNA sensors (or genosensors) have demonstrated to be interesting alternatives to more complex conventional strategies. Currently, electrochemical genosensors are considered very promising analytical tools for this purpose due to their fast response, low cost, high sensitivity, compatibility with microfabrication technology and simple operation mode which makes them compatible with point-of-care (POC) testing. In this review, the relevance and current challenges of the determination of circulating biomarkers related to relevant diseases (cancer, bacterial and viral infections and neurodegenerative diseases) are briefly discussed. An overview of the electrochemical nucleic acid–based strategies developed in the last five years for this purpose is given to show to both familiar and non-expert readers the great potential of these methodologies for circulating biomarker determination. After highlighting the main features of the reported electrochemical genosensing strategies through the critical discussion of selected examples, a conclusions section points out the still existing challenges and future directions in this field.

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Electrochemical bioplatforms for the simultaneous determination of interleukin (IL)-8 mRNA and IL-8 protein oral cancer biomarkers in raw saliva

Cited by 90 publications

References 22 publications

Enzyme-free electrochemical immunosensor based on methylene blue and the electro-oxidation of hydrazine on Pt nanoparticles

Enzyme-free electrochemical immunosensor based on methylene blue and the electro-oxidation of hydrazine on Pt nanoparticles

Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis

Electrochemical Genosensing of Circulating Biomarkers

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