Electrochemical behaviour of anticancer drug lomustine and in situ evaluation of its interaction with DNA

Carvalho, Paulina Andréa Viana de; Lopes, Ilanna Campelo; Silva, Elizaura Hyeda C.; Bruzaca, Evellin Enny S.; Alves, Hugo José; Lima, Mayara Ingrid Sousa; Tanaka, Auro Atsushi

doi:10.1016/j.jpba.2019.112786

Cited by 13 publications

(6 citation statements)

References 24 publications

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“…In voltammetric techniques, the measured electrochemical signal is an algebraic sum of the undesirable capacitive current and the desired current related to the proper electrode reaction, the so-called Faraday current. DPV is an electrochemical technique which is widely used in chemical analysis and for studying the interactions of pharmaceuticals with DNA [ 41 , 43 , 45 , 51 , 60 , 63 , 65 , 94 , 95 ]. Its high sensitivity results from the pulsating change of potential applied to the working electrode.…”

Section: Electrochemical Methods Appliedmentioning

confidence: 99%

“…The most common are glassy carbon electrodes (GCEs) [ 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ], pencil graphite electrodes (PGEs) [ 50 , 51 , 52 , 53 , 54 , 55 , 56 ], carbon paste electrodes (CPEs) [ 57 , 58 ], hanging mercury drop electrodes (HMDEs) [ 59 ], platinum electrodes [ 60 ], and gold electrodes [ 61 , 62 ]. These electrodes can be used either in the bare form [ 44 , 54 , 63 ] or modified with a layer capable of increasing their sensitivity [ 50 , 51 , 52 , 53 , 55 , 56 ].…”

Section: Electrochemical Approach To Dna–drug Interaction Descriptionmentioning

confidence: 99%

“…Such analysis often involves examining the electrochemical behavior of a pharmaceutical in a solution to which DNA is gradually added. Consequently, the impact of the subsequent portions (increasing the DNA concentration) on the redox processes of the examined system is determined [ 24 , 26 , 44 , 46 , 59 , 63 ]. An alternative method is the modification of the electrode, which involves immobilizing one of the analyzed system elements (studied compound with biological activity [ 64 ] or DNA [ 41 , 42 , 57 , 61 , 62 , 63 , 65 , 66 ]) on the surface of the working electrode.…”

Section: Electrochemical Approach To Dna–drug Interaction Descriptionmentioning

confidence: 99%

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What Can Electrochemical Methods Offer in Determining DNA–Drug Interactions?

2021

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The interactions of compounds with DNA have been studied since the recognition of the role of nucleic acid in organisms. The design of molecules which specifically interact with DNA sequences allows for the control of the gene expression. Determining the type and strength of such interaction is an indispensable element of pharmaceutical studies. Cognition of the therapeutic action mechanisms is particularly important for designing new drugs. Owing to their sensitivity, simplicity, and low costs, electrochemical methods are increasingly used for this type of research. Compared to other techniques, they require a small number of samples and are characterized by a high reliability. These methods can provide information about the type of interaction and the binding strength, as well as the damage caused by biologically active molecules targeting the cellular DNA. This review paper summarizes the various electrochemical approaches used for the study of the interactions between pharmaceuticals and DNA. The main focus is on the papers from the last decade, with particular attention on the voltammetric techniques. The most preferred experimental approaches, the electrode materials and the new methods of modification are presented. The data on the detection ranges, the binding modes and the binding constant values of pharmaceuticals are summarized. Both the importance of the presented research and the importance of future prospects are discussed.

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Section: Electrochemical Methods Appliedmentioning

confidence: 99%

Section: Electrochemical Approach To Dna–drug Interaction Descriptionmentioning

confidence: 99%

Section: Electrochemical Approach To Dna–drug Interaction Descriptionmentioning

confidence: 99%

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What Can Electrochemical Methods Offer in Determining DNA–Drug Interactions?

2021

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“…Lomustine is a bifunctional alkylating antineoplastic agent that belongs to the nitrosurea class, and is used to treat brain tumors. The dsDNA in situ interaction with lomustine and with chemically degraded lomustine was investigated in incubated solutions, using dsDNA electrochemical biosensors and comet assays [79]. Lomustine metabolites initially cause condensation of the DNA double helix, followed by DNA unwinding.…”

Section: Alkylating Agentsmentioning

confidence: 99%

DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage

Chiorcea-Paquim

Oliveira-Brett

2021

Sensors

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Deoxyribonucleic acid (DNA) electrochemical biosensors are devices that incorporate immobilized DNA as a molecular recognition element on the electrode surface, and enable probing in situ the oxidative DNA damage. A wide range of DNA electrochemical biosensor analytical and biotechnological applications in pharmacology are foreseen, due to their ability to determine in situ and in real-time the DNA interaction mechanisms with pharmaceutical drugs, as well as with their degradation products, redox reaction products, and metabolites, and due to their capacity to achieve quantitative electroanalytical evaluation of the drugs, with high sensitivity, short time of analysis, and low cost. This review presents the design and applications of label-free DNA electrochemical biosensors that use DNA direct electrochemical oxidation to detect oxidative DNA damage. The DNA electrochemical biosensor development, from the viewpoint of electrochemical and atomic force microscopy (AFM) characterization, and the bottom-up immobilization of DNA nanostructures at the electrode surface, are described. Applications of DNA electrochemical biosensors that enable the label-free detection of DNA interactions with pharmaceutical compounds, such as acridine derivatives, alkaloids, alkylating agents, alkylphosphocholines, antibiotics, antimetabolites, kinase inhibitors, immunomodulatory agents, metal complexes, nucleoside analogs, and phenolic compounds, which can be used in drug analysis and drug discovery, and may lead to future screening systems, are reviewed.

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“…Among them, chemotherapy is still the mainstream method (Huang et al., 2019 ), so it is necessary to restrain the shortcomings of chemotherapy such as low therapeutic effect and multidrug resistance (Mangal et al., 2017 ; Zhao et al, 2017 ). Platinum-based drugs account for 80% of tumor treatment formulas (Rossi & Di Maio, 2016 ; Yu et al., 2020 ), and their therapeutic mechanism are closely concerned with the production of reactive oxygen species (ROS) (Ma et al., 2017 ) which is considered as a mediator of DNA damage (Srinivas et al., 2019 ) and a signal molecule in tumors (Moloney & Cotter, 2018 ; Carvalho et al., 2019 ). Though the low concentration of ROS can promote the proliferation, growth, and differentiation of tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Prodrug-based nano-delivery strategy to improve the antitumor ability of carboplatin in vivo and in vitro

Lang

Li³

et al. 2021

Drug Delivery

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Chemotherapy plays a major role in the treatment of cancer, but it still has great limitations in anti-tumor effect. Carboplatin (CAR) is the first-line drug in the treatment of non-small cell lung cancer, but the therapeutic effect is demonstrated weak. Therefore, we modified CAR with hexadecyl chain and polyethylene glycol, so as to realize its liposolubility and PEGylation. The synthesized amphiphilic CAR prodrugs could self-assemble into polymer micelles in water with an average particle size about 11.8 nm and low critical micelles concentration (0.0538 mg·mL –1 ). In vivo pharmacodynamics and cytotoxicity experiment evidenced that the polymer micelles were equipped with preferable anti-tumor effect, finally attained the aim of elevating anti-tumor effect and prolonging retention time in vivo . The self-assembled micelles skillfully solve the shortcomings of weak efficacy of CAR, which provides a powerful platform for the application of chemical drug in oncology.

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Electrochemical behaviour of anticancer drug lomustine and in situ evaluation of its interaction with DNA

Cited by 13 publications

References 24 publications

What Can Electrochemical Methods Offer in Determining DNA–Drug Interactions?

What Can Electrochemical Methods Offer in Determining DNA–Drug Interactions?

DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage

Prodrug-based nano-delivery strategy to improve the antitumor ability of carboplatin in vivo and in vitro

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