Electrochemical behaviour and voltammetric determination of rosuvastatin calcium in pharmaceutical preparations using a square-wave voltammetric method

Abstract

In this study, the electrochemical behaviour of rosuvastatin calcium, which is a hydroxy methyl glutaryl Co-A inhibitor (a member of the statin group), used for the treatment of hypercholesterolemia and dyslipidemia was investigated using cyclic voltammetry (CV) and chronoamperometry (CA) methods. According to these studies it is assumed that the reaction is a diffusion-controlled process and irreversible. The results from the CA were calculated using Cottrell's equation and the diffusion coefficient was found to be 5.79 Â 10 À5 AE 0.22 Â 10 À5 cm 2 s À1 . It was calculated that 2 electrons were transferred. For the determination of rosuvastatin calcium from the pharmaceutical preparations, a square wave voltammetry (SWV) method was selected and developed because it is more sensitive and faster than the other voltammetric methods. Rosuvastatin calcium's reduction peak was seen at À1184 mV in pH 5 acetate buffer with a hanging mercury drop electrode (HMDE) used as the working electrode, an Ag/AgCl with saturated 3 M KCl reference electrode and a platinum wire counter electrode. 70 Hz frequency, 4 mV scan increment and 25 mV pulse amplitude were chosen as optimum parameters. This method was validated according to the ICH guidelines on analytical method validation processes. Linearity for rosuvastatin calcium was found between 0.20 and 10.00 mg mL À1 . While the limit of detection for rosuvastatin calcium was 0.07 mg mL À1 , the limit of quantitation was 0.20 mg mL À1 . As a result of these validation studies, the selective, accurate and precise square wave voltammetric method, which gives sensitive and repeatable results, was applied to the determination of rosuvastatin calcium from pharmaceutical preparations. The results obtained from the developed method were compared with a spectrophotometric method and a capillary electrophoresis method reported in the literature and no significant difference was found statistically. Fig. 1 Chemical structure of rosuvastatin calcium.

Analytical Methods for the Determination of Rosuvastatin in Pharmaceutical Formulations and Biological Fluids: A Critical Review

“…Other techniques for ROS quantification were also applied, including capillary zone electrophoresis, mercury drop electrode, highly sensitive enzyme-linked immunosorbent assay (ELISA), high-performance thin-layer chromatography (HPTLC), and TLC, as demonstrated in Table 5. [54,[130][131][132][133][134][135][136][137][138] A microwell-based automated spectrophotometric method for determination of drugs using a colored charge-transfer (CT) complex has been developed as an alternative for the conventional spectrophotometric technique. [137][138][139][140] This non-conventional spectrophotometric assay demonstrated advantages, such as automation, lower consumption of organic solvents, and relative sensitivity with the limit of quantification of 7.85 mg/well for the quantification of ROS.…”

Electrochemical determination of an anti-hyperlipidimic drug pitavastatin at electrochemical sensor based on electrochemically pre-treated polymer film modified GCE

“…The reduction peak at −1.31 V vs. Ag/AgCl for PST was an irreversible one, as on reversing the potential no anodic peak corresponding to the reduction process was observed even at positive potential. The irreversible nature of the electrode process was further established by increasing scan rates which resulted in shift of peak potential to negative values with increased peak currents [34] .

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“…The regression data of calibration plot obtained by the developed electrochemical method for the determination of PST are presented in Table 2 . The limit of detection (LOD) and limit of quantification (LOQ) were calculated using the equations [34] , where s is standard deviation and m is slope of the calibration plot. The calculated LOD was found to be 9.66±0.04 nM and LOQ was amounted to be 32.25±0.07 nM.…”

Quantitative Analysis of Rosuvastatin Calcium in Bulk and Solid Pharmaceutical Dosage Forms Using Green and Rapid Fourier-Transform Infrared Spectroscopic Method

“…Similar procedure was applied for the pharmaceutical samples also. 2 mg of sample was taken in 98 mg KBr electrophoresis [56] , Voltammetry: hanging mercury drop electrode [57] , Charge-transfer complex [58] . No doubt these methods are able to deliver desired analytical accuracy but came with added disadvantages too.…”