Electrochemical Behaviors of Glucose Oxidase Based on Biocatalytic Deposition of Gold Nanoparticles

Abstract

Coupling nanotechnology with biocatalysis, a highly sensitive glucose biosensor for the study of electrochemical behaviors of glucose oxidase (GOx) was proposed by using monkshoodvine root-bark like carbon (MLC) as the platform for the biocatalytic deposition of AuNPs. The biosensor showed a linear range from 0.25 to 130 mM with a detection limit of 0.1 mM (S/N = 3) towards glucose and sensitivity of 3010 mA/mM. K M app value was calculated to be 67.4 mM. Furthermore, the proposed AuNPs/GOx-MLC modified pyrolytic graphite electrode (AuNPs/GOx-MLC/PGE) achieved direct electron transfer of GOx. G* was calculated to be 2.79 × 10 -11 mol/cm 2 and k s was 1.79 s -1 . It also showed a remarkable electrocatalysis towards glucose.