Electrochemical Behavior of Catechol and 3,4-Dihydroxytoluene in Acetonitrile at a Platinum-Disk Electrode Modified with a Tyrosinase Containing Polyacrylamide Film

Miyasaka, Takahiro; Takahashi, Yuriko; Nakamura, Toshio

doi:10.2116/analsci.17.1055

Cited by 7 publications

(8 citation statements)

References 11 publications

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“…A number of cation [8,9] and anion [10] selective electrodes and biosensors [11] were developed at our laboratory using a non-plasticized hydrophilic poly(acrylamide) (PAA) as membrane matrix for use in dipolar aprotic solvents and in the study of the thermodynamic properties of ions. Studies of the response mechanisms of the electrodes were also carried out by voltammetry and spectrophotometry [12,13].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical characterization of poly(acrylamide) membrane coated on a gold disk electrode in acetonitrile

Ji¹,

Jin²,

Ren³

et al. 2005

Journal of Electroanalytical Chemistry

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The electrochemical behavior of a poly(acrylamide) (PAA) membrane electrode (ME), in which the PAA works as a membrane matrix for use in nonaqueous solutions, was investigated in acetonitrile. It was found that PAA MEs possess properties of microelectrode ensembles (MEEs).For the thin PAA membrane, the electrode behaved similar to a macroelectrode and peak-shaped voltammograms were achieved at low scan rates, while at high scan rates the electrode acted as a microelectrode and sigmoidal-shaped voltammograms were obtained. For the thick PAA membrane electrode, however, voltammograms were sigmoidal-shaped at low scan rates, and at high scan rates the voltammograms became peak-shaped again. Quantitative and semiquantitative analyses of the electrochemical response characteristics of these ensembles were conducted. The results show that the electrochemical behavior of these ensembles is in close agreement with the predictions of established microwell electrochemical theory.

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Section: Introductionmentioning

confidence: 99%

Electrochemical characterization of poly(acrylamide) membrane coated on a gold disk electrode in acetonitrile

Ji¹,

Jin²,

Ren³

et al. 2005

Journal of Electroanalytical Chemistry

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“…8 In the absence of substrates, both the tyrosinase, NG-MTP-Au and the NG-MTP-Au electrode gave no detectable signal. And after the substrate addition into the cell, no response was also observed either at the bare gold electrode ( Fig.…”

Section: Cyclic Voltammetric Measurementsmentioning

confidence: 97%

“…The reduction potential was almost the same as that obtained at the tyrosinase immobilized polyacrylamide (PAA) electrode in AN. 8 The 1,2-benzoquinone was produced from the enzymatic oxidation of catechol with dissolved oxygen at the tyrosinase modified electrode (Scheme 2) and it was reduced electrically at the nano-gold surface, and the electron transfer occurred through MTP to give a reduction current on cyclic voltammograms.…”

Section: Cyclic Voltammetric Measurementsmentioning

confidence: 99%

“…A tyrosinase-immobilized polyacrylamide membrane platinum electrode has been prepared and the electrochemical behavior of o-hydroxybenzene derivatives in acetonitrile 8 and dimethylacetamide 9 has been studied. Because of unique properties of nanoparticles, recent investigations have focused on the development of enzyme-modified nanoparticle-based electrochemical biosensors.…”

Section: Introductionmentioning

confidence: 99%

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Application of the Nanogold-4,4′-bis(methanethiol)biphenyl Modified Gold Electrode to the Determination of Tyrosinase-Catechol Reaction Kinetics in Acetonitrile

et al. 2006

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Many studies on the use of enzymatic activity in nonaqueous solutions have been carried out after Dastoli and Price's report of their investigation of the catalytic activity of xanthine oxidase suspended in several organic media. 1 Updike and Hicks published the results of their experiment involving an enzyme electrode with cross-linked polyacrylamide (PAA) membrane as matrix for use in aqueous solutions.2 Enzymes have been found to have numerous applications as practical and specific catalysts in chemical and pharmaceutical syntheses and as recognition elements in biosensors. The discovery that they are catalytically active in organic solvents containing little or no water has expanded their repertoire of use still further. 3,4 Some of the benefits in utilizing enzymes in nonaqueous solvents over the use of conventional aqueous reaction media include the high solubility of many hydrophobic substrates in such solvents, the suppression of various side reactions promoted by water, altered enzymatic selectivity and the ability to control it with the solvent, and the comparative simplicity of immobilization procedures due to the insolubility of enzymes in organic solvents. Many studies have been conducted in order to gain an understanding of so-called solvent effects due to organic solvent properties. Factors such as solvent hydrophobicity, 5 solvent polarity, water content, 6 and substrate hydrophobicity 7 can affect the enzymatic reaction. It remains important to continue the search for new enzymatic reaction systems because of the use which might be made of such systems in both pure and applied research.Many enzyme-based electrochemical biosensors have been described for use in both aqueous and nonaqueous solutions. A tyrosinase-immobilized polyacrylamide membrane platinum electrode has been prepared and the electrochemical behavior of o-hydroxybenzene derivatives in acetonitrile 8 and dimethylacetamide 9 has been studied. Because of unique properties of nanoparticles, recent investigations have focused on the development of enzyme-modified nanoparticle-based electrochemical biosensors. [10][11][12] The ability of nanoparticles to stabilize enzymes, and to improve the efficiency of immobilized enzymes is extremely useful when preparing biosensors. Moreover, other characteristics of nanoparticles, such as their high surface-to-volume ratio, their high surface energy, and their ability to decrease the distance between enzyme and metal particles, may facilitate electron transfer between redox sites and the electrode surfaces.In our laboratory, a gold nanoparticle self-assembled electrode has been constructed by using 4,4′-bis(methanethiol)biphenyl (MTP), rigid rod dithiols, as a binder between a gold disk and gold nanoparticles. The effect of gold nanoparticles on the interaction of Co(phen)3 3+ with DNA, and the characteristics of the electrode in a nonaqueous solution were described in a recent report. 13 We have also devised a method of preparing density-controlled gold nano-particle self-assembled monolayer (SAM...

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“…18 An immobilization of functional materials utilized for tracing reactions is also described in this paper. A tyrosinase(Tyro)-immobilized poly(acrylamide) (PAA) membrane platinum disk electrode was prepared and the electrochemical behaviors of o-hydroxybenzene derivatives in acetonitrile (AN) 19 and N,Ndimethylacetamide (DMA) 20 were reported. Electrochemical characterization of PAA membrane coated on a gold disk electrode in AN was carried out, and it was shown that the membrane electrode has array properties.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensors in Nonaqueous Solutions and Their Applications

Nakamura

2007

ANAL. SCI.

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This review first describes the invention of functional interfaces to promote biochemical redox reactions between substrates in dipolar aprotic solvents and enzymes or related compounds immobilized at the interface. The interfaces contain hydrophilic polymer membranes, a gold nanoparticle self-assembled electrode constructed by using rigid rod dithiols, and binary self-assembled monolayers composed of amino and carboxyl terminal groups. Other topics covered are: the electrochemical characterization of the hydrophilic polymer membrane; the development of biosensors to obtain reaction parameters of enzymatic and of electrochemical kinetics; and applications to the study of materials involved in metabolism.

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Electrochemical Behavior of Catechol and 3,4-Dihydroxytoluene in Acetonitrile at a Platinum-Disk Electrode Modified with a Tyrosinase Containing Polyacrylamide Film

Cited by 7 publications

References 11 publications

Electrochemical characterization of poly(acrylamide) membrane coated on a gold disk electrode in acetonitrile

Electrochemical characterization of poly(acrylamide) membrane coated on a gold disk electrode in acetonitrile

Application of the Nanogold-4,4′-bis(methanethiol)biphenyl Modified Gold Electrode to the Determination of Tyrosinase-Catechol Reaction Kinetics in Acetonitrile

Electrochemical Biosensors in Nonaqueous Solutions and Their Applications

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