Electrochemical aptasensor for the detection of tetracycline with multi-walled carbon nanotubes amplification

Zhou, Lu; Li, Du-Juan; Gai, Ling; Wang, Jianping; Li, Yanbin

doi:10.1016/j.snb.2011.12.067

Cited by 159 publications

(66 citation statements)

References 44 publications

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“…The anti-TET oligonucleotides purchased from Shanghai Sangon Biological Engineering Technology & Services Co., Ltd. (Shanghai, China) with the following sequences were designed by Niazi [34] and Zhou [23]: 5'-NH 2 -(CH 2 )-CGT ACG GAA TTC GCT AGC CCC CCG GCA GGC CAC GGC TTG GGT TGG TCC CAC TGC GCG TGG ATC CGA GCT CCA CGT G -3' and probe aptamer 5'-GCA TGC CTT AAG CGA TCG GGG GGC CGT CCG GTG CCG AAC CCA ACC AGG GTG ACG CGC ACC TAG GCT CGA GGT GCA C-6 FAM (FITC)-3' Chloroauric acid (HAuCl 4 ) and ethanol were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Chitosan (CS) was purchased from Sangon Biotech Co., Ltd. (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

“…In recent years, new rapid electrochemical methods for TET detection appeared frequently, such as immunosensors [21], enzyme sensors [22] and aptasensor [23]. Aptamers are nucleic acid ligands selected from random sequence pools in vitro, which can be naturally folded into different three-dimensional structures and have the capability of binding specifically to biosurfaces [24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

“…Compared to antibodies, aptamers have more advantages, such as low cost, stability, simplicity, easy modification, target versatility and can be synthetically manufactured. To the best of our knowledge, electrochemical aptasensors for TET detection have been applied to detect TET in the analysis of the food safety [23,28]. Kim et al [28] developed a electrochemical aptasensor based on the screen printed gold electrode using square-wave voltammetry (SWV) technique for the detection of TET, but the proposed aptasensor suffered the drawbacks of low sensitivity, and the stability and reproducibility of the method were not discussed.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Aptasensor Based on Prussian Blue-Chitosan-Glutaraldehyde for the Sensitive Determination of Tetracycline

et al. 2014

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Abstract:In this paper, a novel and sensitive electrochemical aptasensor for detecting tetracycline (TET) with prussian blue (PB) as the label-free signal was fabricated. A PB-chitosan-glutaraldehyde (PB-CS-GA) system acting as the signal indicator was developed to improve the sensitivity of the electrochemical aptasensor. Firstly, the PB-CS-GA was fixed onto the glass carbon electrode surface. Then, colloidal gold nanoparticles (AuNPs) were droped onto the electrode to immobilize the anti-TET aptamer for preparation of the aptasensor. The stepwise assembly process of the aptasensor was characterized by cyclic voltammetry (C-V) and scanning electron microscope (SEM). The target TET captured onto the electrode induced the current response of the electrode due to the non-conducting biomoleculars. Under the optimum operating conditions, the response of differential pulse voltammetry (DPV) was used for detecting the concentration of TET. The proposed aptasensor showed a high sensitivity and a wide linear range of 10 −9 ∼ 10 −5 M and 10 −5 ∼ 10 −2 M with the correlation coefficients of 0.994 and 0.992, respectively. The detection limit was 3.2×10 −10 M (RSD 4.12%). Due to its rapidity, sensitivity and low cost, the proposed aptasensor could be used as a pre-scanning method in TET determination for the analysis of livestock products.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrochemical Aptasensor Based on Prussian Blue-Chitosan-Glutaraldehyde for the Sensitive Determination of Tetracycline

et al. 2014

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“…The decrease in DPV current at the electrode surface caused by the interaction with the analyte was used to quantify the content of TCs in milk samples. Another configuration involving the immobilization of the aptamer onto a MWCNTs/GCE and measurement of the decrease in the DPV response of [Fe(CN) 6 ] 3−/4− after complexation was reported [140]. Chen et al [135] constructed an impedimetric aptasensor by immobilizing an amino-modified aptamer on a gold electrode.…”

Section: Electrochemical Affinity Biosensors For Drug Residuesmentioning

confidence: 99%

Electrochemical Affinity Biosensors in Food Safety

2017

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Safety and quality are key issues of today's food industry. Since the food chain is becoming more and more complex, powerful analytical methods are required to verify the performance of food safety and quality systems. Indeed, such methods require high sensitivity, selectivity, ability for rapid implementation and capability of automatic screening. Electroanalytical chemistry has, for decades, played a relevant role in food safety and quality assessment, taking more and more significance over time in the solution of analytical problems. At present, the implementation of electrochemical methods in the food is evident. This is in a large part due to the relevant results obtained by combining the attractive advantages of electrochemical transduction strategies (in terms of relatively simple hardware, versatility, interface with automatic logging and feasibility of application outside the laboratory environment) with those from biosensors technology. Important examples of enzyme electrochemical biosensors are those dedicated to the determination of glucose, alcohol or cholesterol are important examples. In addition, other types of different electrochemical biosensing approaches have emerged strongly in the last years. Among these, the strategies involving affinity interactions have been shown to possess a large number of applications. Therefore, electrochemical immunosensors and DNA-based biosensors have been widely used to determine major and minor components in foodstuffs, providing sufficient data to evaluate food freshness, the quality of raw materials, or the origin of samples, as well as to determine a variety of compounds at trace levels related to food safety such as micotoxins, allergens, drugs residues or pathogen microorganisms. This review discusses some critical examples of the latest advances in this area, pointing out relevant methodologies related to the measurement techniques, including the use of nanostructured electrodes and strategies for signal amplification.

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“…Generally, CNTs are applied in electrochemical sensing of ECPs in the following modes: directly modifying the electrode with CNTs to improve the response [71,72]; hybridization of CNTs with other materials (e.g., AuNPs [73], SbNPs [74], ionic liquid [75], graphene [76], dye [77], and organometallic compound [78]) to achieve synergetic effects; modifying the CNTs or using them together with other recognizing units (e.g., MIP [73], antibody [75], aptamer [79], b-CD [80], and melamine [81]) to improve the selectivity of the sensor.…”

Section: Electrochemical Sensors Based On Carbon Nanomaterialsmentioning

confidence: 99%