Electrochemical approach of anticancer drugs–DNA interaction

Rauf, Sakandar; Gooding, J. Justin; Akhtar, Kalsoom; Ghauri, Muhammad Afzal; Rahman, Md Jamilur; Anwar, Munir A.; Khalid, Ahmad Mukhtar

doi:10.1016/j.jpba.2004.10.037

Cited by 290 publications

(129 citation statements)

References 84 publications

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“…1,2 However mechanism of interactions between drug molecules and DNA is still relatively little known. It is necessary to introduce more simple methods for investigating the mechanism of interaction.…”

Section: Introductionmentioning

confidence: 99%

Study on the interaction between doxorubicin and Deoxyribonucleic acid with the use of methylene blue as a probe

Hajian

Shams

Mohagheghian

2009

J. Braz. Chem. Soc.

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Neste trabalho, a interação de doxorrubicina com DNA (obtido de timo de bezerro) em fita dupla foi investigada através de técnicas de espectrofotometria UV-Vis, voltametria e espectrofluorometria, usando azul de metrileno (MB) como marcador. O comportamento voltamétrico da doxorrubicina foi investigado em eletrodo de carbono vítreo usando voltametria de pulso diferencial. A doxorrubicina é reduzida, produzindo um pico de redução. Os dois estudos, espectrofotometria UV-Vis e voltametria de pulso diferencial, confirmam a reação de intercalação. Os resultados mostraram que a doxorrubicina e a molécula de MB foram intercaladas na dupla hélice do DNA. A constante de ligação aparente de doxorrubicina com DNA foi 3,2 × 10 4 L mol -1 . O sinal de fluorescência da doxorrubicina e azul de metileno é suprimido com a adição de DNA. A equação de Stern-Volmer baseou-se na supressão do sinal de fluorescência da doxorrubicina.In this work, the interaction of doxorubicin with calf thymus double strand Deoxyribonucleic acid (ds-DNA) has been investigated with the use of Methylene Blue (MB) dye as a probe by the application of UV-Vis spectrophotometry, voltammetry and spectrofluorometry. The voltammetric behavior of doxorubicin has been investigated at glassy carbon electrode using differential pulse voltammetry. Doxorubicin is reduced, yielding one reduction peak. Both UV-Vis spectrophotometry and differential pulse voltammetry studies confirm the intercalation reaction. The results showed that both doxorubicin and the MB molecule could intercalate into the double helix of the DNA. The apparent binding constant of doxorubicin with DNA has been found to be 3.2 × 10 4 L mol -1 . The fluorescence signal of doxorubicin and methylene blue was quenched with DNA addition. The Stern-Volmer equation was plotted based on quenching fluorescence signal of doxorubicin.Keywords: doxorubicin, DNA, chemotherapy, spectrophotometry, voltammetry, spectrofluorometry IntroductionStudy of interactions between drugs and DNA is very interesting and significant not only in understanding the mechanism of interaction, but also for the design of new drugs. 1,2 However mechanism of interactions between drug molecules and DNA is still relatively little known. It is necessary to introduce more simple methods for investigating the mechanism of interaction. By understanding the mechanism of interaction, designing of new DNA-targeted drugs and the screening of these in vitro will be possible.A great variety of substances, including several agents of importance in cancer chemotherapy, 3 are known to bind to DNA by intercalation. 4 Attention has been concentrated on the classical intercalating drugs, acridines and ethidium bromide. [4][5][6] Studies on the binding of various dyes, drugs and antibiotics to DNA and chromatin have contributed to the understanding of the structure of these macromolecules, 6-14 and have suggested possible mechanisms of the biological activity of some drugs. 3 Molecular models of the intercalation of some drugs into DNA have been describ...

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Section: Introductionmentioning

confidence: 99%

Study on the interaction between doxorubicin and Deoxyribonucleic acid with the use of methylene blue as a probe

Hajian

Shams

Mohagheghian

2009

J. Braz. Chem. Soc.

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“…Interaction between DNA and drug molecules is of current general interest and importance, 1,2 especially for the designing of new DNA-targeted drugs and the screening of these in vitro.…”

Section: Introductionmentioning

confidence: 99%

Study on the interaction between morin-bi(III) complex and DNA with the use of methylene blue dye as a fluorophor probe

Ensafi

Hajian

Ebrahimi

2009

J. Braz. Chem. Soc.

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Com base em nossa investigação, ambos os complexos, morin-Bi(III) e Morin, podem vincularse ao DNA, embora a natureza da ligação seja diferente para cada um deles. Na presença e ausência do DNA, o morin-Bi(III) mostrou características espectrais diferentes, o que está de acordo com as observadas para outros intercaladores. Neste trabalho, a interação do complexo morin-Bi(III) com o DNA de timo de vitela foi investigada com o uso do azul de metileno (MB), como uma sonda de corante espectral e aplicação de espectrofotometria UV-Vis, espectroscopia de fluorescência e voltametria cíclica. , enquanto que o morin liga-se por um modelo de não-intercalação.Based on our investigation, although both morin-Bi(III) complex and morin can bind to DNA, the nature of the binding was found to be different for each of them. In the presence and absence of the DNA, the morin-Bi(III) complex shows different spectral characteristics which agree with those observed for other intercalators. In this work, the interaction of morin-Bi(III) complex with calf thymus DNA was investigated with the use of methylene blue (MB) dye as a spectral probe and application of UV-Vis spectrophotometry, fluorescence spectroscopy and cyclic voltammetry. The 2:1 morin-Bi(III) complex ratio was calculated by UV-Vis spectroscopy (mole ratio method). The fluorescence signal of Bi(III)-morin complex is increased with DNA addition whereas the fluorescence signal of Morin is decreased with DNA addition. The fluorescence signal of the DNA-complex is quenched by addition of MB which confirms the displacement of the complex with MB. Cyclic voltammetry studies confirm the intercalation reaction. The results showed that only morin-Bi(III) complex can intercalate into the double helix of the DNA. The apparent binding constant of morin-Bi(III) complex with DNA is found to be 2.8 × 10 4 L mol -1, while morin binds in a non-intercalation mode.

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“…Different types of electrode materials have been used for the investigation of drug-DNA interaction such as carbon paste electrode, gold electrode, pencil graphite electrode, glassy carbon electrode and screen printed electrodes. Drug-DNA interactions have been investigated with electrochemical techniques such as cyclic voltammetry, square wave voltammetry, differential pulse voltammetry and chronopotentiometry 25 . The mechanism of interaction can be investigated in three different ways: 1.…”

Section: Dna Interaction Studiesmentioning

confidence: 99%

Development of Electrochemical DNA Biosensors-A Review

2015

Chem Sci Trans

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Electrochemical DNA biosensors are used widely due to its sensitivity, accessibility and accuracy. This electrochemical era in DNA biosensors took almost three decades for its development and application. Purine bases in the DNA namely guanine and adenine oxidizes at the working electrode during application of a positive potential. This property of DNA has been utilized in electrochemical DNA biosensors in various fields from the clinical diagnostics, biomedical, forensic applications till environmental studies. With the use of nanotechnology, various nanomaterials have been utilized over the past years in order to increase the sensitivity of the biosensor. A clear understanding on the DNA immobilization strategy is required for sensor preparation for its various applications. A detailed account on the initial developments till current scenario in electrochemical DNA biosensors have been provided in order to understand the basics of electrochemical DNA biosensor and its application in developing a DNA biosensor.

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Electrochemical approach of anticancer drugs–DNA interaction

Cited by 290 publications

References 84 publications

Study on the interaction between doxorubicin and Deoxyribonucleic acid with the use of methylene blue as a probe

Study on the interaction between doxorubicin and Deoxyribonucleic acid with the use of methylene blue as a probe

Study on the interaction between morin-bi(III) complex and DNA with the use of methylene blue dye as a fluorophor probe

Development of Electrochemical DNA Biosensors-A Review

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