Electrochemical Analysis of Glycoprotein Samples Prepared on a Pneumatically‐controlled Microfluidic Device

Abstract

In this work, we designed and manufactured a microfluidic device for isolation and purification of glycoprotein samples. The conceived sample preparation device was fabricated in polycarbonate by micro‐milling. The flow control and the fluid dosage into the micro‐channels was solved by equipping the device with integrated pneumatic valves. The biochemical functionality was provided by beaded support modified by molecules with affinity to glycoproteins which was stacked inside the micro‐channel reminiscent of packed affinity columns used in glycoprotein lectin assays. Unlabeled glycoproteins, namely fetuin, asialofetuin, and prostate‐specific antigen, were voltammetrically analyzed using catalytic peak H at silver amalgam electrode.

Common Post-translational Modifications (PTMs) of Proteins: Analysis by Up-to-Date Analytical Techniques with an Emphasis on Barley

“…The columns, injection channel, ESI tip, and connections are housed on a laminated polyimide chip, which is inserted into the ChipCube interface in a credit-card-sized stainless-steel holder, with the Infinity LC instrument serving as the solvent delivery system. In 2019 Novotný et al 53 introduced a microfluidic device for isolation and purification of glycoprotein samples. As seen in Figure 3 , the microfluidic chip contained a bed of microbeads coupled to immobilized biomolecules that served as a bioaffinity column.…”

“…Which fluid is injected is determined by the valves and CDA-manifold. Reprinted with permission from ref ( 53 ). Copyright 2019 John Wiley and Sons.…”

Use of Lectin-based Affinity Techniques in Breast Cancer Glycoproteomics: A Review

“…Recently, an alternative approach where LAC is miniaturized to a microfluidic format has also been reported and has also become very popular for glycan and glycoproteins analysis. Commercial or custom fabricated miniaturized devices are profitable for both analytical and economic reasons (reduced sample and reagent consumption, elimination of contamination, and improved reproducibility and detection limits). − …”