Electrocardiography and heart rate variability in Göttingen Minipigs: Impact of diurnal variation, lead placement, repeatability and streptozotocin-induced diabetes

Lyhne, Mille Kronborg; Debes, Karina Poulsdóttir; Helgogaard, Terese; Vegge, Andreas; Pedersen‐Bjergaard, Ulrik; Olsen, Lisbeth H.

doi:10.1016/j.vascn.2022.107221

Cited by 3 publications

(2 citation statements)

References 46 publications

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“…In our previous study using the QT/RR plot analysis of microminipig [ 4 ], Fridericia’s formula corrected the QT interval for the heart rate better than Van de Water’s or Bazett’s one. The same result has been reported for some miniature swine including Göttingen [ 8 ] and NIBS [ 6 ]. Since appropriate correction formula has not been studied for Clawn miniature swine, in this study we adopted Fridericia’s formula for microminipig as well as Clawn miniature swine.…”

supporting

confidence: 86%

Characterization of the electrocardiogram of <i>microminipig</i> in comparison with that of Clawn miniature swine: impacts of miniaturization of body size on electrocardiographic indices

Goto

Kambayashi

Saito

et al. 2023

The Journal of Veterinary Medical Science

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Effects of body size reduction on electrocardiographic indices were examined using microminipigs in comparison with Clawn miniature swine (Clawn). Electrocardiogram was recorded using Holter electrocardiograph in conscious state for 24 hr for microminipigs (male: 11.6 ± 0.1 kg, 12–17 months, n=5; and female: 9.9 ± 0.4 kg, 6 months, n=5) and Clawn (female: 20.3 ± 0.4 kg, 8–9 months, n=8). Microminipig had shorter PR interval and QRS width than Clawn, whereas no significant difference was detected in JTcF/QTcF between them. Ratios of PR interval, QRS width, and body weight cubic root for microminipigs to Clawn ranged between 0.713 and 0.830. These findings indicate that PR interval and QRS width will depend on distance for excitatory current propagation, whereas JTcF/QTcF may be governed by local electrical activities.

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supporting

confidence: 86%

Characterization of the electrocardiogram of <i>microminipig</i> in comparison with that of Clawn miniature swine: impacts of miniaturization of body size on electrocardiographic indices

Goto

Kambayashi

Saito

et al. 2023

The Journal of Veterinary Medical Science

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“…However, the naturally low concentrations of cfDNA require substantial volumes of blood, making some of the most used models, such as rodent models, impractical. Established pig models, such as the Göttingen minipig, are thus an attractive alternative for studying the role of cfDNA in cardiometabolic disease [ 16–22 ].…”

Section: Introductionmentioning

confidence: 99%

Nanopore sequencing reveals methylation changes associated with obesity in circulating cell-free DNA from Göttingen Minipigs

et al. 2023

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Profiling of circulating cell-free DNA (cfDNA) by tissue-specific base modifications, such as 5-methylcytosines (5mC), may enable the monitoring of ongoing pathophysiological processes. Nanopore sequencing allows genome-wide 5mC detection in cfDNA without bisulphite conversion. The aims of this study were: i) to find differentially methylated regions (DMRs) of cfDNA associated with obesity in Göttingen minipigs using Nanopore sequencing, ii) to validate a subset of the DMRs using methylation-specific PCR (MSP-PCR), and iii) to compare the cfDNA DMRs with those from whole blood genomic DNA (gDNA). Serum cfDNA and gDNA were obtained from 10 lean and 7 obese Göttingen Minipigs both with experimentally induced myocardial infarction and sequenced using Oxford Nanopore MinION. A total of 1,236 cfDNA DMRs (FDR<0.01) were associated with obesity. In silico analysis showed enrichment of the adipocytokine signalling, glucagon signalling, and cellular glucose homoeostasis pathways. A strong cfDNA DMR was discovered in PPARGC1B , a gene linked to obesity and type 2 diabetes. The DMR was validated using MSP-PCR and correlated significantly with body weight ( P < 0.05). No DMRs intersected between cfDNA and gDNA, suggesting that cfDNA originates from body-wide shedding of DNA. In conclusion, nanopore sequencing detected differential methylation in minute quantities (0.1–1 ng/µl) of cfDNA. Future work should focus on translation into human and comparing 5mC from somatic tissues to pinpoint the exact location of pathology.

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Pharmacokinetics and pharmacodynamics of standard nerve agent medical countermeasures in Göttingen Minipigs

Langston,

Moffett,

Pennington

et al. 2024

Toxicology Letters

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Electrocardiography and heart rate variability in Göttingen Minipigs: Impact of diurnal variation, lead placement, repeatability and streptozotocin-induced diabetes

Cited by 3 publications

References 46 publications

Characterization of the electrocardiogram of <i>microminipig</i> in comparison with that of Clawn miniature swine: impacts of miniaturization of body size on electrocardiographic indices

Characterization of the electrocardiogram of <i>microminipig</i> in comparison with that of Clawn miniature swine: impacts of miniaturization of body size on electrocardiographic indices

Nanopore sequencing reveals methylation changes associated with obesity in circulating cell-free DNA from Göttingen Minipigs

Pharmacokinetics and pharmacodynamics of standard nerve agent medical countermeasures in Göttingen Minipigs

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