Electrocardiogram Recordings in Anesthetized Mice using Lead II

Ha, Tae Woong; Oh, Bermseok; Kang, Ji‐One

doi:10.3791/61583

Cited by 4 publications

(3 citation statements)

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“…To address these issues and refine the recording of ECGs in zebrafish, we adapted the PowerLab system, previously used for recording ECGs in mice and rats ( Adriaens et al, 2018 ; Ha et al, 2020 ; Kim et al, 2019 ; Meo et al, 2016 ; Zhu et al, 2020 ). We integrated this system with components dissembled from the iWorx ECG system and fabricated components as follows.…”

Section: Resultsmentioning

confidence: 99%

Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

Duong

Rose

Blazeski

et al. 2021

Disease Models &Amp; Mechanisms

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Clinically pertinent electrocardiogram (ECG) data from model systems, such as zebrafish, are crucial for illuminating factors contributing to human cardiac electrophysiology abnormalities and disease. Current zebrafish ECG collection strategies have not adequately addressed the consistent acquisition of high-quality traces or sources of phenotypic variation that could obscure data interpretation. Thus, we developed a novel platform to ensure high-quality recording of in vivo subdermal adult zebrafish ECGs and zERG (Zebrafish ECG Reading GUI), a program to acquire measurements from traces commercial software cannot examine due to erroneous peak calling. We evaluate normal ECG trait variation, revealing the intervals are highly reproducible while wave amplitude variation appears largely driven by recording artifacts, and identify sex and body size as potential confounders to PR, QRS, and QT intervals. With this framework, we characterize the effect of the class I anti-arrhythmic drug flecainide acetate on adults, provide support for the impact of a Long QT syndrome model, and establish power calculations for this and other studies. These results highlight our pipeline as a robust approach to evaluate zebrafish models of human cardiac electrophysiologic phenotypes.

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Section: Resultsmentioning

confidence: 99%

Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

Duong

Rose

Blazeski

et al. 2021

Disease Models &Amp; Mechanisms

View full text Add to dashboard Cite

show abstract

“…After the optimal anesthesia level was achieved, the mice were placed on a heated thermal plate in the supine position to obtain ECG recordings. For Lead II, the needle ECG electrodes were located under the skin of the right arm and the left leg ( Figure 2 ) [ 21 ]. Surface ECG recordings were obtained using PowerLab/SP8 (ADInstruments, Australia) with an ECG sampling frequency of 4 kHz.…”

Section: Methodsmentioning

confidence: 99%

Classical heart rate variability and non-linear heart rate analysis in mice under Na-Pentobarbital and Ketamine/Xylazine anesthesia.

KAZDAĞLI¹,

ÖZEL²,

ÖZBEK³

et al. 2022

Turkish Journal of Medical Sciences

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Background/aim Anesthetics are often used in animal experiments to achieve immobilization and relieve pain. However, many anesthetics can alter the dynamics of cardiovascular systems. We aimed to compare the effects of two frequently used anesthetics agents on heart rate variability (HRV) parameters in mice. Materials and methods This observational study was performed between May and June 2014 in 21 male BALB/c mice aged 16–20 weeks. The animals were divided into three groups: pentobarbital (P), (n = 7); pentobarbital+fentanyl (P+F), (n = 7); and ketamine+xylazine (K+X), (n = 7). Surface electrocardiography (ECG) electrodes were placed in lead II configuration. The tachogram of RR intervals was obtained after R waves were detected using the Pan-Tompkins real-time QRS recognition algorithm. Frequency-domain, time-domain, and nonlinear HRV analyses were performed. Results The bradycardia effect was higher in the K+X group (p < 0.01). Time-domain indices were higher in group K+X compared to group P (p < 0.01) and group P+F (p < 0.001). Very low frequency (VLF) power was significantly lower in group K+X compared to group P and group P+F (p < 0.01). Low frequency (LF) power, low frequency/high frequency (LF/HF) ratio, and total power (TP) were higher in group K+X compared to group P (p < 0.01) and group P+F (p < 0.001). The detrended fluctuation analysis short-term parameter (DFAα 1 ) was significantly higher in group K+X compared to group P+F (p < 0.05) and the long-term parameter (DFAα 2 ) was lower in group K+X compared to group P (p < 0.05). Standard deviations SD1 and SD2 were higher in group K+X compared to group P (p < 0.001) and group P+F (p < 0.001), SD2/SD1 ratio was lower in group K+X compared to group P (p < 0.05) and group P+F (p < 0.05). Entropy measures did not differ between groups. Conclusion HRV analyses, including nonlinear methods, indicated that a K+X combination reduces imbalance and disorder in the regulation of the autonomic nervous system (ANS) in comparison to both P and the P+F combination.

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“…In small animals, including rodents and rabbit, the ECG is frequently recorded in general anesthesia ( Ha, Oh, and Kang, 2020 ). In anesthetized animals, needle electrodes are usually placed under the skin of thorax or limbs.…”

Section: Ecg Recordingmentioning

confidence: 99%

Golden Standard or Obsolete Method? Review of ECG Applications in Clinical and Experimental Context

et al. 2022

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Cardiovascular system and its functions under both physiological and pathophysiological conditions have been studied for centuries. One of the most important steps in the cardiovascular research was the possibility to record cardiac electrical activity. Since then, numerous modifications and improvements have been introduced; however, an electrocardiogram still represents a golden standard in this field. This paper overviews possibilities of ECG recordings in research and clinical practice, deals with advantages and disadvantages of various approaches, and summarizes possibilities of advanced data analysis. Special emphasis is given to state-of-the-art deep learning techniques intensely expanded in a wide range of clinical applications and offering promising prospects in experimental branches. Since, according to the World Health Organization, cardiovascular diseases are the main cause of death worldwide, studying electrical activity of the heart is still of high importance for both experimental and clinical cardiology.

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Electrocardiogram Recordings in Anesthetized Mice using Lead II

Cited by 4 publications

References 0 publications

Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafish

Classical heart rate variability and non-linear heart rate analysis in mice under Na-Pentobarbital and Ketamine/Xylazine anesthesia.

Golden Standard or Obsolete Method? Review of ECG Applications in Clinical and Experimental Context

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