Electroantennogram and Single Sensillum Recording in Insect Antennae

Olsson, Shannon B.; Hansson, Bill S.

doi:10.1007/978-1-62703-619-1_11

Cited by 89 publications

(64 citation statements)

References 28 publications

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“…6 E and F and SI Methods and Materials), however, the antenna responded to mechanical (air puffs) and olfactory (lavender oil) stimuli, showing the adequacy of the present electrophysiological preparation. These responses were consistent with previously reported antennal sensitivity to mechanical and olfactory stimulation (20).…”

Section: Bumblebee Hairs Exhibit Neural Correlates To DC Electric Fieldsupporting

confidence: 93%

Mechanosensory hairs in bumblebees ( Bombus terrestris ) detect weak electric fields

Sutton

Clarke

Morley

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Bumblebees (Bombus terrestris) use information from surrounding electric fields to make foraging decisions. Electroreception in air, a nonconductive medium, is a recently discovered sensory capacity of insects, yet the sensory mechanisms remain elusive. Here, we investigate two putative electric field sensors: antennae and mechanosensory hairs. Examining their mechanical and neural response, we show that electric fields cause deflections in both antennae and hairs. Hairs respond with a greater median velocity, displacement, and angular displacement than antennae. Extracellular recordings from the antennae do not show any electrophysiological correlates to these mechanical deflections. In contrast, hair deflections in response to an electric field elicited neural activity. Mechanical deflections of both hairs and antennae increase with the electric charge carried by the bumblebee. From this evidence, we conclude that sensory hairs are a site of electroreception in the bumblebee.electric fields | bees | behavior | sensory biology

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Section: Bumblebee Hairs Exhibit Neural Correlates To DC Electric Fieldsupporting

confidence: 93%

Mechanosensory hairs in bumblebees ( Bombus terrestris ) detect weak electric fields

Sutton

Clarke

Morley

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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“…A further technique for investigating the olfactory response of insects towards semiochemicals utilizes single sensillum recordings. Here, the electrical activity of one sensillum is measured rather than all sensilla on the antennal flagellum . This method, alone and in combination with GC, has been used for decades in aphid olfaction research and more recently has been applied successfully in psyllid olfaction research .…”

Section: Identification and Evaluation Of Plant‐emitted Whitefly Semimentioning

confidence: 89%

Prospects for management of whitefly using plant semiochemicals, compared with related pests

Schlaeger

Pickett

Birkett

2018

Pest Management Science

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Whitefly (Hemiptera: Sternorrhyncha: Aleyrodidae) pests, including the tobacco whitefly, Bemisia tabaci, and the greenhouse whitefly, Trialeurodes vaporariorum, are important economically in agriculture. Whiteflies are controlled mainly by synthetic insecticides but resistance to these is evolving rapidly. A semiochemical‐based management strategy could provide an alternative to the use of insecticides, by exploiting natural volatile signalling processes to manipulate insect behaviour. Whitefly behaviour is affected by differences in plant odour blends. Selected compounds have been suggested as putative semiochemicals, but in only a few studies have potential volatiles been characterized by electrophysiology or olfactometry. Application of antennal preparation methods from closely related families, the aphids (Hemiptera: Aphididae) and psyllids (Hemiptera: Psyllidae), may help to facilitate whitefly electroantennography. Behavioural bioassays are essential to identify the repellent or attractant effect of each semiochemical. The relevance of semiochemicals in whitefly management needs to be evaluated in the respective cultivation system. Although the value of semiochemicals against whiteflies has not been demonstrated in the field, there is an emerging range of possible field applications and some promising prospects. Overall, the olfactory system of whiteflies needs to be elucidated in more detail. © 2018 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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“…Antennae were excised and both ends were trimmed with a scalpel, randomizing whether right or left antennae were excised first. Trimmed antennae were then attached to glass capillary reference and recording electrodes filled with insect saline solution (210 mM NaCl, 3.1 mM KCl, 10 mM CaCl 2 , 2.1 mM NaCO 3 , 0.1 NaH 2 PO 4 ) as previously described44. EAG responses were recorded on the EAD program of a Syntech™ IDAC-4 signal acquisition unit.…”

Section: Methodsmentioning

confidence: 99%

Odorant cues linked to social immunity induce lateralized antenna stimulation in honey bees (Apis mellifera L.)

McAfee

Collins

Madilao

et al. 2017

Sci Rep

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Hygienic behaviour (HB) is a social immunity trait in honey bees (Apis mellifera L.) whereby workers detect, uncap and remove unhealthy brood, improving disease resistance in the colony. This is clearly economically valuable; however, the molecular mechanism behind it is not well understood. The freeze-killed brood (FKB) assay is the conventional method of HB selection, so we compared odour profiles of FKB and live brood to find candidate HB-inducing odours. Surprisingly, we found that significantly more brood pheromone (β-ocimene) was released from FKB. β-ocimene abundance also positively correlated with HB, suggesting there could be a brood effect contributing to overall hygiene. Furthermore, we found that β-ocimene stimulated worker antennae in a dose-dependent manner, with the left antennae responding significantly stronger than right antennae in hygienic bees, but not in non-hygienic bees. Five other unidentifiable compounds were differentially emitted from FKB which could also be important for HB. We also compared odour profiles of Varroa-infested brood to healthy brood and found an overall interactive effect between developmental stage and infestation, but specific odours did not drive these differences. Overall, the data we present here is an important foundation on which to build our understanding the molecular mechanism behind this complex behaviour.

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Electroantennogram and Single Sensillum Recording in Insect Antennae

Cited by 89 publications

References 28 publications

Mechanosensory hairs in bumblebees ( Bombus terrestris ) detect weak electric fields

Mechanosensory hairs in bumblebees ( Bombus terrestris ) detect weak electric fields

Prospects for management of whitefly using plant semiochemicals, compared with related pests

Odorant cues linked to social immunity induce lateralized antenna stimulation in honey bees (Apis mellifera L.)

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