A new biomimetic sensor for leucomalachite green host-guest interactions and potentiometric transduction is presented. e arti�cial host was imprinted in methacrylic acid or acrylamido-2-methyl-1-propanesulfonic acid-based polymers. Molecularly imprinted particles were dispersed in 2-nitrophenyloctyl ether and trapped in poly(vinyl chloride). e potentiometric sensors exhibited a near-Nernstian response in steady state evaluations, with slopes and detection limits ranging from 45.8 to 81.2 mV decade −1 and 0.28 to 1.01 g mL −1 , respectively. ey were independent from the pH of test solutions within 3 to 5. Good selectivity was observed towards drugs that may contaminate water near �sh cultures, such as oxycycline, doxycycline, enro�oxacin, trimethoprim, creatinine, chloramphenicol, and dopamine. e sensors were successfully applied to �eld monitoring of leucomalachite green in river samples. e method offered the advantages of simplicity, accuracy, applicability to colored and turbid samples, and automation feasibility.