Electroanalytical Method for Determination of Trace Metals in Struvite Using Electrochemically Treated Screen-Printed Gold Electrodes

Vasconcellos, Maria de Lourdes S.; Rocha, Diego P.; Castro, S.; Silva, Luiz; Muñoz, Rodrigo A.A.; Freitas, M.B.J.G.; Ferreira, Rafael de Queiroz

doi:10.21577/0103-5053.20200081

Cited by 6 publications

(1 citation statement)

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“…The activation of the modified electrode was performed by 10 cycles in 1 mol L −1 H 2 SO 4 solution to eliminate impurities that can hinder the adsorption of molecules, diminishing the reproducibility and stability of the modified surface [45][46][47].…”

Section: Electrodeposition Of Gold Nanoparticles On Carbon Electrodesmentioning

confidence: 99%

Development of an Electrochemical Immunosensor for Specific Detection of Visceral Leishmaniasis Using Gold-Modified Screen-Printed Carbon Electrodes

et al. 2020

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Visceral leishmaniasis is a reemerging neglected tropical disease with limitations for its diagnosis, including low concentration of antibodies in the serum of asymptomatic patients and cross-reactions. In this context, this work proposes an electrochemical immunosensor for the diagnosis of visceral leishmaniasis in a more sensitive way that is capable of avoiding cross-reaction with Chagas disease (CD). Crude Leishmania infantum antigens tested in the enzyme-linked immunosorbent assay (ELISA) were methodologically standardized to best engage to the sensor. The antibodies anti-Trypanosoma cruzi and anti-Leishmania sp. Present in serum from patients with diverse types of CD or leishmaniasis were chosen. A screen-printed carbon electrode modified with gold nanoparticles was the best platform to guarantee effective adsorption of all antigens so that the epitope of specific recognition for leishmaniasis occurred efficiently and without cross-reaction with the evaluated CD. The current peaks reduced linearly after the recognition, and still were able to notice the discrimination between different kinds of diseases (digestive, cardiac, undetermined Chagas/acute and visceral chronic leishmaniasis). Comparative analyses with ELISA were performed with the same groups, and a low specificity (44%) was verified due to cross-reactions (high number of false positives) on ELISA tests, while the proposed immunosensor presented high selectivity and specificity (100%) without any false positives or false negatives for the serum samples from isolated patients with different types of CD and visceral leishmaniasis. Furthermore, the biosensor was stable for 5 days and presented a detection limit of 200 ng mL−1.

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Section: Electrodeposition Of Gold Nanoparticles On Carbon Electrodesmentioning

confidence: 99%

Development of an Electrochemical Immunosensor for Specific Detection of Visceral Leishmaniasis Using Gold-Modified Screen-Printed Carbon Electrodes

et al. 2020

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A portable electroanalytical procedure to determine paracetamol in organic fertilizers

Silva

Vasconcellos

Ferreira

2022

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Portable and sustainable electrochemical platform for the sub-minute detection of norfloxacin in organic fertilizers

Silva,

Stefano,

de Lourdes Soprani Vasconcellos

et al. 2023

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Electroanalytical Method for Determination of Trace Metals in Struvite Using Electrochemically Treated Screen-Printed Gold Electrodes

Cited by 6 publications

References 0 publications

Development of an Electrochemical Immunosensor for Specific Detection of Visceral Leishmaniasis Using Gold-Modified Screen-Printed Carbon Electrodes

Development of an Electrochemical Immunosensor for Specific Detection of Visceral Leishmaniasis Using Gold-Modified Screen-Printed Carbon Electrodes

A portable electroanalytical procedure to determine paracetamol in organic fertilizers

Portable and sustainable electrochemical platform for the sub-minute detection of norfloxacin in organic fertilizers

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