Electroanalysis of Biomolecules: Rational Selection of Sensor Construction

Shumyantseva, Victoria V.; Agafonova, L.E.; Булко, Т. В.; Kuzikov, Alexey V.; Masamrekh, Rami A.; Yuan, Jiayin; Pergushov, Dmitry V.; Sigolaeva, Larisa V.

doi:10.1134/s0006297921140108

Cited by 9 publications

(7 citation statements)

References 64 publications

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“…We assume that the specific characteristics of MWCNTs, such as strong adsorptive property and high specific surface area, combined with the advantages of PILs, such as high dispersing power, contribute to the efficient adsorption of dsDNA onto the SPE and therefore to sensitivity of the dsDNA assay. Such a synergistic effect of MWCNTs combined with an ionic amphiphilic (micelle-forming) diblock copolymer was already reported by us previously with respect to the quantitative electrochemical detection of myoglobin [45]. The herein described easy-preparable PIL/MWCNT-modified SPEs compared to other electrochemical setups intended for the dsDNA quantification exhibit a similar or better sensitivity for the electrochemical oxidation of dsDNA [26,46].…”

Section: Discussionsupporting

confidence: 76%

Long-term stable poly(ionic liquid)/MWCNTs inks enable enhanced surface modification for electrooxidative detection and quantification of dsDNA

Sigolaeva

Булко

Kozin

et al. 2019

Polymer

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This work demonstrates the use of imidazolium-based poly(ionic liquid)s (PILs) as efficient dispersants of multi-walled carbon nanotubes (MWCNTs). With these polymeric dispersants, highly stable fine dispersions of MWCNTs (inks) can be easily prepared in aqueous media and applied for rather simple but efficient surface modification of screen-printed electrodes (SPEs). Such a modification of SPEs remarkably increases the electroactive surface area and accelerates the electron transfer rate due to synergistic combination of specific features of MWCNTs such as strong adsorptive property and high specific surface with the advantages of PILs like ion conductivity and dispersability. We further show that the PIL/MWCNT-modified SPEs can be beneficially utilized for direct electrochemical analysis of double stranded DNA (dsDNA). Specifically, it is exemplified by the direct electrooxidation of guanine and adenine bases in salmon testes dsDNA chosen as a model system. The linear ranges for the determination of dsDNA correspond to 5-500 µg/mL for the oxidative peak of guanine and 0.5-50 µg/mL for the oxidative peak of adenine. This makes direct electrochemical dsDNA detection with the use of the easy-preparable PIL/MWCNT-modified SPEs strongly competing to currently applied spectral and fluorescent techniques. Furthermore, we show that the developed constructs are capable of sensing a single point mutation in the 12-bases single-stranded DNA fragments. Such detection is of high clinical significance in choosing an adequate anticancer treatment, where the electrochemical identification of the point mutation could offer time and cost benefits.

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Section: Discussionsupporting

confidence: 76%

Long-term stable poly(ionic liquid)/MWCNTs inks enable enhanced surface modification for electrooxidative detection and quantification of dsDNA

Sigolaeva

Булко

Kozin

et al. 2019

Polymer

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“…This type of electrode makes it possible to work with biological objects without the stage of sensor regeneration. Modification of the electrode surface with nanoparticles (electrode nanostructuring) makes it possible to select the optimal conditions, adjust the sensor to the selected electrochemical reaction, the substance to be determined, and provide the necessary analytical characteristics of the method [19][20][21][22][23][24][25]. In this work, we used electrodes modified with single-walled carbon nanotubes, stabilized with carboxymethylcellulose.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Studies of the Interaction of Phospholipid Nanoparticles with dsDNA

et al. 2022

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The effect of phospholipid nanoparticles with different contents of phosphatidylcholine (PhNP80 and PhNP100) on dsDNA was studied by means of the electrochemical method. Changes in the electrochemical behavior of heterocyclic bases guanine, adenine and thymine in the range of potentials of 0.2–1.2 V in the presence of PhNPs were used for the assessment of the binding mechanism of the ligand–DNA interaction. Comparative analysis of the effect of PhNPs with different contents of phosphatidylcholine showed a more pronounced effect on the dsDNA of the PhNP100 nanosystem. From the obtained experimental data on the decrease in the amplitude of the nucleobases' electrochemical oxidation currents, the electrochemical coefficient of the toxic effect was calculated as the ratio of the electrooxidation currents of dsDNA and dsDNA in the presence of phospholipid nanoparticles. PhNP80/100 (up to 11.4 mg/mL) does not influence dsDNA, PhNP80/100 (14.3–28.5 mg/mL) has a moderate toxic effect on dsDNA, PhNP80/100 at concentrations above 28.5 mg/mL already have a toxic effect, significantly reducing the maximum amplitude of the heterocyclic bases’ electrochemical oxidation current. Peak potentials of electrochemical oxidation of nucleobases did not shift in the presence of PhNP80 and PhNP100 (in the concentration range of 2.3–42.2 mg/mL), which could be possible through a groove-binding mode of phospholipid nanoparticle interaction with dsDNA.

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“…• rational design of sensor structures and recording circuits using various modifications of electrodes to enhance the signal [143,144].…”

Section: Electrochemical Analysis Of Surface Antigenic Proteins and P...mentioning

confidence: 99%

Perspectives for the creation of a new type of vaccine preparations based on pseudovirus particles using polio vaccine as an example

Zhdanov,

Ivin,

Shishparenok

et al. 2023

BIOMED KHIM

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Traditional antiviral vaccines are currently created by inactivating the virus chemically, most often using formaldehyde or β-propiolactone. These approaches are not optimal since they negatively affect the safety of the antigenic determinants of the inactivated particles and require additional purification stages. The most promising platforms for creating vaccines are based on pseudoviruses, i.e., viruses that have completely preserved the outer shell (capsid), while losing the ability to reproduce owing to the destruction of the genome. The irradiation of viruses with electron beam is the optimal way to create pseudoviral particles. In this review, with the example of the poliovirus, the main algorithms that can be applied to characterize pseudoviral particles functionally and structurally in the process of creating a vaccine preparation are presented. These algorithms are, namely, the analysis of the degree of genome destruction and coimmunogenicity. The structure of the poliovirus and methods of its inactivation are considered. Methods for assessing residual infectivity and immunogenicity are proposed for the functional characterization of pseudoviruses. Genome integrity analysis approaches, atomic force and electron microscopy, surface plasmon resonance, and bioelectrochemical methods are crucial to structural characterization of the pseudovirus particles.

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Electroanalysis of Biomolecules: Rational Selection of Sensor Construction

Cited by 9 publications

References 64 publications

Long-term stable poly(ionic liquid)/MWCNTs inks enable enhanced surface modification for electrooxidative detection and quantification of dsDNA

Long-term stable poly(ionic liquid)/MWCNTs inks enable enhanced surface modification for electrooxidative detection and quantification of dsDNA

Electrochemical Studies of the Interaction of Phospholipid Nanoparticles with dsDNA

Perspectives for the creation of a new type of vaccine preparations based on pseudovirus particles using polio vaccine as an example

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