Electroanalysis of amino acid substitutions in bioengineered acetylcholinesterase

Biological Activity of sym-Triazines with Acetylcholine-like Substitutions as Multitarget Modulators of Alzheimer’s Disease

“…After each measurement, screen-printed electrodes were disposed of, thereby preventing contamination by electrode material from previous measurements. 24,25 Electrochemical characterization of AChE activity was further validated by Ellman's colorimetric assay.…”

High content of pyridinic- and pyrrolic-nitrogen-modified carbon nanotubes derived from blood biomass for the electrocatalysis of oxygen reduction reaction in alkaline medium

“…Interestingly, the synthesis of these materials can be derived from several biological proteins, such as peptides [8], hemoglobin [9] and collagen [10]. In recent years, approaches have included the study of oxygen reduction reaction behavior in many nitrogen-doped electrocatalysts utilizing N-enriched amino acids as the N sources or metal macrocyclic complexes contained inside hemin biomaterials as effective precursors [11][12][13][14][15]. Although they have superior ORR performance, their stability still can't meet the requirements for practical application in renewable energy.…”

Electroenzymatic model system for the determination of catalytic activity of Erwinia carotovora L- asparaginase

“…Estimation of ASNase catalytic activity is based on different approaches, such as determination of L-Asn content or ammonia concentration measured by high-performance liquid chromatography (HPLC), direct amino acid quanti cation by circular dichroism (CD) (Kudryashova & Sukhoverkov 2016), electrophoresis assays, and determination by a colorimetric assay from complexation with hydroxylamine (Magri et al 2018). The simultaneous production of L-aspartic acid (L-Asp) and NH (Somji et al 2012). Amino acid substitutions introduced through site-directed mutagenesis of AChE were detected using square-wave voltammetry on a screen-printed carbon electrode.…”

“…Amino acid substitutions introduced through site-directed mutagenesis of AChE were detected using square-wave voltammetry on a screen-printed carbon electrode. The authors underlined that complex conformational changes of the polypeptide chain have a more dominant in uence on the oxidation pro le of the mutants than the individual amino acid substitutions (Somji et al 2012).…”

“…In our investigation, the ability and sensitivity of amino acids of the polypeptide backbone to conformational changes were used to estimate the catalytic activity of ASNase. We assumed that in the course of catalytic conversion of L-Asn as a substrate, structural uctuations of ASNase occurred, which led to the rearrangement of the protein chain and changes in the intensities of electroactive amino acids as speci c electrochemical labels (Somji et al 2012). These properties of protein can be registered with voltammetric techniques such as differential pulse voltammetry.…”