Electroacupuncture Ameliorates Abnormal Defaecation and Regulates Corticotrophin-Releasing Factor in a Rat Model of Stress

Zhao, Yong; Changxiang, Cui; Yu, Xiao-Chun; Xin, Juan-Juan; Lu, Fanghong; Gao, Junhong; Zhu, Bing

doi:10.1136/acupmed-2016-011080

Cited by 10 publications

(11 citation statements)

References 27 publications

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“…CRHR1 and CRH elevation induced by hepatectomy was alleviated by EA. The same result was also found in cold stress (Eshkevari et al, 2013 ), chronic heterotypic stress (Zhao et al, 2017 ) and CUMS (Le et al, 2016 ), which demonstrated a role for EA in the HPA axis regulation.…”

Section: Discussionsupporting

confidence: 66%

Electroacupuncture Alleviates Surgical Trauma-Induced Hypothalamus Pituitary Adrenal Axis Hyperactivity Via microRNA-142

Zhu

Chen

Meng

et al. 2017

Front. Mol. Neurosci.

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Electroacupuncture (EA) could improve the hyperactivity of the hypothalamus pituitary adrenal (HPA) axis induced by hepatectomy. However, its underlying mechanism still remains largely unclear. Here, we found that hypothalamic corticotrophin releasing hormone (CRH) modulates the function of the HPA axis, while hepatectomy induced an HPA axis disorder and EA application could regulate the hypothalamic CRH. We first demonstrated that microRNAs (miRNAs) target on CRH via bioinformatics analysis and screened them in the primary hypothalamic neurons. MicroR-142 (miR-142) and miR-376c were identified to inhibit CRH at the mRNA and protein levels, and a dual luciferase reporter assay confirmed their binding to the 3′-untranslated regions (3′-UTR) of CRH. Further analyses revealed a decrease in hypothalamic miR-142 expression in the hepatectomy rats and an increase in miR-142 and miR-376c after EA intervention. Importantly, the improvement effect of EA on the HPA axis regulatory function in hepatectomy rats was blocked by miR-142 antagomir. Our findings illustrated that EA could up-regulate hypothalamic miR-142 expression and decrease the CRH level to alleviate the hyperactivity of the HPA axis induced by hepatectomy.

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Section: Discussionsupporting

confidence: 66%

Electroacupuncture Alleviates Surgical Trauma-Induced Hypothalamus Pituitary Adrenal Axis Hyperactivity Via microRNA-142

Zhu

Chen

Meng

et al. 2017

Front. Mol. Neurosci.

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“…Acupoints of ST36 (Zusanli) on bilateral hind limbs were used for EA treatment. 29 The acupoint of ST36 in rats is located 10 mm below the knee joint and 5 mm lateral to the anterior tubercle of the tibia. Acupuncture needles at each hind limb were inserted as follows: one into the ST36 acupoint and the other one 5 mm below the ST36 acupoint (5 mm in depth).…”

Section: Ea Treatmentmentioning

confidence: 99%

<p>Electroacupuncture Treatment Attenuates Paclitaxel-Induced Neuropathic Pain in Rats via Inhibiting Spinal Glia and the TLR4/NF-κB Pathway</p>

Zhao¹,

Yao²,

Li³

et al. 2020

JPR

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Background and Purpose: Neuropathic pain is a major side-effect of paclitaxel (PTX) chemotherapy. Although the precise mechanisms responsible for this pain are unclear, the activation of neuroglia and upregulation of the TLR4/NF-κB pathway are known to be involved. In this study, we determined whether electroacupuncture (EA) could limit mechanical hypersensitivity resulting from the chemotherapeutic drug PTX in rats, and investigated the potential mechanisms involved. Methods: Rats intraperitoneally received a cumulative dose of 8 mg/kg PTX (2 mg/kg per day) or vehicle control on alternate days (day 0, 2, 4 and 6). EA treatment (10 Hz, 1 mA) was applied at bilateral ST36 acupoints in rats once every other day on days 0-14. For sham EA, needles were inserted at ST36 acupoints without electrical stimulation. Mechanical allodynia was measured by mechanical withdrawal latency (MWL) of paws to a mechanical stimulus every 2 days. Protein expression of TLR4 and NF-κB p65, as well as TMEM119 and GFAP (indicators of microglia and astrocytes, respectively) in spinal cord was quantified by Western blot analysis. Levels of inflammatory cytokines IL-1β and TNF-α in spinal cord and serum were detected by ELISA. Results: Mechanical allodynia induced by PTX in both paws (right and left) of rats was significantly attenuated by EA but not sham EA treatment. In addition, EA, but not sham EA, inhibited the activation of both microglia (TMEM119) and astrocytes (GFAP) in lumbar spinal cord. Moreover, Western blot analysis revealed that protein expression of TLR4 and NF-κB in spinal cord was suppressed by EA but not sham EA treatment. PTX significantly increased inflammatory cytokines in spinal cord and serum, which were ameliorated by EA treatment but not by sham EA. Conclusion: These results indicate that EA treatment attenuates PTX-induced mechanical allodynia. The putative mechanism corroborating this finding could be related to the suppression of activated microglia and astrocytes in spinal cord, as well as the inhibition of the activated TLR4/NF-κB signaling pathway by EA treatment.

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“…Rats in each group were consciously restrained in a wooden holder. As described in our previous study, 15 EA treatment was applied at ST36 in the bilateral hind limbs of rats, located 10 mm below the knee joint and 5 mm lateral to the anterior tubercle of the tibia. Acupuncture needles were inserted bilaterally at ST36 to a depth of 5 mm and connected to an EA apparatus (HANS-200A).…”

Section: Ea Proceduresmentioning

confidence: 99%

“…Interestingly, electroacupuncture (EA) has been reported to have a regulatory role in the modulation of CRF receptors in the amygdala and hypothalamus. 13,14 In addition, our previous study showed that EA significantly attenuated stress-induced abnormal defecation in a rat model of chronic stress; 15 Sprague-Dawley (SD) rats were exposed to chronic heterotypic stress (CHS), which involved a combination of different types of stressors and resulted in accelerated colorectal motility with excessive defecation that was ameliorated by EA at ST36 through inhibition of central and peripheral CRF signals. However, there is limited research focusing on stress-induced alterations in the small intestine, and the possible role and mechanisms of action of EA in the regulation of such dysmotility.…”

Section: Introductionmentioning

confidence: 99%

Electroacupuncture ameliorates corticotrophin-releasing factor-induced jejunal dysmotility in a rat model of stress

et al. 2020

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Background Central injection of corticotrophin-releasing factor (CRF) mimics the effect of stress on gastrointestinal (GI) responses, including inhibition of GI motility. This study was designed to explore the effects of electroacupuncture (EA) on disordered jejunal motility in a rat model of stress induced by intracisternal (IC) injection of CRF. Methods A stress model was established by IC injection of CRF in Sprague–Dawley rats. GI motility was evaluated by assessing gastric emptying (GE), gastrointestinal transit (GIT) and jejunal motility in vivo. EA was performed at ST36. The functional roles of CRF receptor subtype 1 and subtype 2 (CRFr1 and CRFr2) were examined by IC administration of the corresponding selective CRF antagonists. Protein expression of CRFr1 and CRFr2 in the hypothalamus and jejunum was detected by Western blotting. Results IC injection of CRF significantly inhibited GE, GIT and jejunal motility. EA treatment remarkably improved the disturbed GI motility. Intriguingly, the disordered jejunal motility induced by central CRF was abolished by IC injection of a selective CRFr2 antagonist, indicating the essential role of central CRFr2 in mediating the stress-induced jejunal motor disorder. EA at ST36 decreased central and peripheral expression of CRFr2, which might be one of the potential mechanisms underlying the beneficial effect of EA on jejunal dysmotility in this rat model of stress. Conclusion This study suggested that EA at ST36 could ameliorate disordered jejunal motility induced by stress, and that this might be associated with the down-regulation of CRFr2.

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Electroacupuncture Ameliorates Abnormal Defaecation and Regulates Corticotrophin-Releasing Factor in a Rat Model of Stress

Cited by 10 publications

References 27 publications

Electroacupuncture Alleviates Surgical Trauma-Induced Hypothalamus Pituitary Adrenal Axis Hyperactivity Via microRNA-142

Electroacupuncture Alleviates Surgical Trauma-Induced Hypothalamus Pituitary Adrenal Axis Hyperactivity Via microRNA-142

<p>Electroacupuncture Treatment Attenuates Paclitaxel-Induced Neuropathic Pain in Rats via Inhibiting Spinal Glia and the TLR4/NF-κB Pathway</p>

Electroacupuncture ameliorates corticotrophin-releasing factor-induced jejunal dysmotility in a rat model of stress

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