Electroacupuncture alleviates nerve injury after cerebra ischemia in rats through inhibiting cell apoptosis and changing the balance of MMP-9/TIMP-1 expression

Ma, Rong; Yuan, Bo; Du, Junhui; Wang, Lina; Ma, Louyan; Liu, Songfang; Shu, Qing; Sun, Hongzhi

doi:10.1016/j.neulet.2016.09.033

Cited by 16 publications

(5 citation statements)

References 22 publications

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“…To date, it has been shown that after birth in the rat brain, Timp-1 mRNA expression significantly decreased (Fager and Jaworski 2000 ). Similarly, downregulation of Timp-1 mRNA and protein expression was observed in Sprague Dawley rats with ischemic-reperfusion (Ma et al 2016 ). In patients with end-stage neurological disease, chronic activation of astrocytes by IL-1β caused downregulation of TIMP-1 mRNA and protein expression in CSF and brain tissues (Gardner and Ghorpade 2003 ).…”

Section: Discussionmentioning

confidence: 79%

Impact of Elastin-Derived Peptide VGVAPG on Matrix Metalloprotease-2 and -9 and the Tissue Inhibitor of Metalloproteinase-1, -2, -3 and -4 mRNA Expression in Mouse Cortical Glial Cells In Vitro

2018

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Degradation products of elastin, i.e. elastin-derived peptides (EDPs), are involved in various physiological and pathological processes. EDPs are detectable in cerebrospinal fluid in healthy people and in patients after ischemic stroke. However, to date, no studies concerning the role of EDP in the nervous system were conducted. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) play important roles during the repair phases of cerebral ischemia, particularly during angiogenesis and reestablishment of cerebral blood flow. Therefore, the aim of this study was to investigate the impact of the specific elastin-derived peptide VGVAPG on Mmp-2, -9 and Timp-1, -2, -3 and -4 mRNA expression in mouse cortical glial cells in vitro. Primary glial cells were maintained in DMEM/F12 without phenol red supplemented with 10% fetal bovine serum and the cells were exposed to 50 nM, 1 and 50 μM of the VGVAPG peptide. After 3 and 6 h of exposition to the peptide, expression of Mmp-2, -9 and Timp-1, -2, -3 and -4 mRNA was measured. Moreover, siRNA gene knockdown, cytotoxicity and apoptosis measurement were included in our experiments, which showed that VGVAPG in a wide range of concentrations exhibited neither proapoptotic nor cytotoxic properties in mouse glial cells in vitro. The peptides enhanced mRNA expression of Timp-2 and Timp-3 genes in an elastin-binding protein (EBP)-dependent manner. However, changes in mRNA expression of Mmp-2, Mmp-9 and Timp-4 were partially EBP-dependent. The decrease in mRNA expression of Timp-1 was EBP-independent. However, further studies underlying the VGVAPG peptide's mechanism of action in the nervous system are necessary.

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Section: Discussionmentioning

confidence: 79%

Impact of Elastin-Derived Peptide VGVAPG on Matrix Metalloprotease-2 and -9 and the Tissue Inhibitor of Metalloproteinase-1, -2, -3 and -4 mRNA Expression in Mouse Cortical Glial Cells In Vitro

2018

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“…MMP-2/MMP-9 expression was suppressed by EA treatment in brain ischemic rats and EA may alleviate learning and memory dysfunction as well as anatomical damage in post-stroke rats (Lin et al, 2016). 2 Hz EA at Baihui and Siguan markedly elevated the expression of tissue inhibitors of metalloproteinases-1 (TIMP-1) and reduced the expression of matrix metalloproteinase−9 (MMP-9) at protein and mRNA levels, leading to a balance disorder of MMP-9/TIMP-1expression, which might be involved in neuroprotection caused by EA at the Siguan and Baihui acupoints against ischemic brain injury (Ma et al, 2016).…”

Section: Literature Research Criteriamentioning

confidence: 99%

Mechanisms Involved in the Neuroprotection of Electroacupuncture Therapy for Ischemic Stroke

Xing

Zhang

et al. 2018

Front. Neurosci.

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Stroke is one of the main causes of death all over the world. As the combination of acupuncture and electric stimulation, electroacupuncutre is a safe and effective therapy, which is commonly applied in ischemic stroke therapy in both experimental studies and clinical settings. The review was performed via searching for related articles in the databases of OVID, PUBMED, and ISI Web of Science from their respective inceptions to May 2018. In this review, we summarized the mechanism of EA for ischemic stroke via a series of factors, consisting of apoptosis related-factors, inflammatory factors, autophagy-related factors, growth factors, transcriptional factors, cannabinoid CB1 receptors, and other factors. In summary, EA stimulation may effectively alleviate ischemic brain injury via a series of signal pathways and various other factors.

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“…Hegu (LI4) and Taichong (LR3), known as Siguan, are the classical acupoints for relieving pain and inducing resuscitation. Previous studies have found that LI4 and LR3 exerted a neuroprotective role for the central system, manifested as repairing neurons, alleviating nerve inflammation, and inhibiting cell apoptosis [54][55][56]. Functional magnetic resonance imaging (fMRI) studies also found that stimulating LI4 and LR3 could transform the pain-activated brain regions to the inhibitory state, exert analgesic effects, and relieve physical pain caused by central sensitization [57,58].…”

Section: Discussionmentioning

confidence: 99%

Acupoints for Tension-Type Headache: A Literature Study Based on Data Mining Technology

Hao

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Objectives. This study aimed to explore the characteristics and principles of acupoints, which were applied for treating tension-type headache (TTH). Methods. Four databases were searched for the literature studies of treating TTH with acupuncture and moxibustion up to September 1, 2020. Titles, journals, authors, key words, interventions, main acupoints, and outcomes of the included literature studies were extracted and inputted into the self-established Data Excavation Platform of Acupoint Specificity for analysis. Results. In total, 128 papers containing 137 prescriptions, 89 meridian acupoints, and 7 extraordinary acupoints of treating TTH with acupuncture and moxibustion were included. The total frequency of acupoints’ application was 763 times. Fengchi (GB20), Baihui (GV20), Taiyang (EX-HN5), Hegu (LI4), and Taichong (LR3) were used most frequently. The acupoints in Yang meridians were utilized more than those in Yin meridians (66.1% vs. 17.8%), and the acupoints in the Gallbladder Meridian of Foot Shaoyang were applied most commonly. 59.9% (457/763) of the applied acupoints were on the head, face, and neck, and 31.7% (242/763) were on the four limbs. Additionally, the proportion of specific acupoints’ application was 78.2% (597/763). Conclusions. The prescription of Fengchi (GB20), Baihui (GV20), Taiyang (EX-HN5), Hegu (LI4), and Taichong (LR3) might be relatively reasonable in clinical practices of treating TTH with acupuncture, which should be verified in further studies.

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Electroacupuncture alleviates nerve injury after cerebra ischemia in rats through inhibiting cell apoptosis and changing the balance of MMP-9/TIMP-1 expression

Cited by 16 publications

References 22 publications

Impact of Elastin-Derived Peptide VGVAPG on Matrix Metalloprotease-2 and -9 and the Tissue Inhibitor of Metalloproteinase-1, -2, -3 and -4 mRNA Expression in Mouse Cortical Glial Cells In Vitro

Impact of Elastin-Derived Peptide VGVAPG on Matrix Metalloprotease-2 and -9 and the Tissue Inhibitor of Metalloproteinase-1, -2, -3 and -4 mRNA Expression in Mouse Cortical Glial Cells In Vitro

Mechanisms Involved in the Neuroprotection of Electroacupuncture Therapy for Ischemic Stroke

Acupoints for Tension-Type Headache: A Literature Study Based on Data Mining Technology

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