Electrokinetic phenomena have their fundamental significance and have found their wide applications for manipulating fluid flows, particles and cells in microfluidics. However the electrokinetic phenomena in confined microfluidic domains have been received less attention. The phenomenon of particle transport in confined microfluidic I would like to thank Mr. Yuan Kee Hock, the technician in charge of Thermal and Fluids Research Laboratory for purchase matters. Also technical guidance and assistance in microfabrication from the research staff, technical staff and research students at Micro-Machines Centre (MMC) are much appreciated. Furthermore, I would extend my gratitude to Nanyang Technological University for offering me the Ph.D. scholarship and providing me with the opportunity to come to Singapore and pursue my interests in research. Finally, I would like to express my gratitude to my parents and parents-in-law. A special appreciation is given to my son Liang Xuanming and my wife Ms. Wen Yadan, a Ph.D. candidate in Yong Loo Lin School of Medicine, National University of Singapore, for their continuous love, encouragement and support in the past four years. Without them, I definitely cannot have reached where I am now.