Electro-fermentation: Sustainable bioproductions steered by electricity

Virdis, Bernardino; Hoelzle, Robert D.; Marchetti, Angela; Boto, Santiago T.; Blasco-Gómez, Ramiro; Puig, Sebastià; Freguia, Stefano; Villano, Marianna

doi:10.1016/j.biotechadv.2022.107950

Cited by 46 publications

(21 citation statements)

References 205 publications

(250 reference statements)

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“…Pyruvate can either re-enter the TCA cycle to potentially enhance the acetoin production or accumulate in cells to trigger the acetoin excretion as a strategy for cells to maintain the intercellular pH during the stationary phase [ 45 ]. Alternatively, the applied anode potential may also change the oxidation–reduction potential (ORP) of the fermentation broth or the intracellular redox state, the principle of which is still to be discovered [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Anode-assisted electro-fermentation with Bacillus subtilis under oxygen-limited conditions

Sun

Kokko

Vassilev

2023

Biotechnol Biofuels

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Background Bacillus subtilis is generally regarded as a ubiquitous facultative anaerobe. Oxygen is the major electron acceptor of B. subtilis, and when oxygen is absent, B. subtilis can donate electrons to nitrate or perform fermentation. An anode electrode can also be used by microorganisms as the electron sink in systems called anodic electro-fermentation. The facultative anaerobic character of B. subtilis makes it an excellent candidate to explore with different electron acceptors, such as an anode. This study aimed to optimise industrial aerobic bioprocesses using alternative electron acceptors. In particular, different end product spectrum of B. subtilis with various electron acceptors, including anode from the electro-fermentation system, was investigated. Results B. subtilis was grown using three electron acceptors, i.e. oxygen, nitrate and anode (poised at a potential of 0.7 V vs. standard hydrogen electrode). The results showed oxygen had a crucial role for cells to remain metabolically active. When nitrate or anode was applied as the sole electron acceptor anaerobically, immediate cell lysis and limited glucose consumption were observed. In anode-assisted electro-fermentation with a limited aeration rate, acetoin, as the main end product showed the highest yield of 0.78 ± 0.04 molproduct/molglucose, two-fold higher than without poised potential (0.39 ± 0.08 molproduct/molglucose). Conclusions Oxygen controls B. subtilis biomass growth, alternative electron acceptors utilisation and metabolites formation. Limited oxygen/air supply enabled the bacteria to donate excess electrons to nitrate or anode, leading to steered product spectrum. The anode-assisted electro-fermentation showed its potential to boost acetoin production for future industrial biotechnology applications.

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Section: Discussionmentioning

confidence: 99%

Anode-assisted electro-fermentation with Bacillus subtilis under oxygen-limited conditions

Sun

Kokko

Vassilev

2023

Biotechnol Biofuels

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“…This type of direct communication with biofilms would be in contrast to electrogenetic systems 48 , in which electronic communication is achieved with planktonic cells via a soluble mediator. Delivery of excess reducing equivalents to cells also has important implications for microbial electrosynthesis 49 and electrofermentation 50 . Although wild type M. atlanticus biofilms have been shown to take up electrons for O 2 reduction when configured as a cathode 17 , more work is needed to understand where electrons go and how this could be harnessed using synthetic biology.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A living conductive marine biofilm engineered to sense and respond to small molecules

Bird

Leary

Hervey

et al. 2022

Preprint

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Engineered electroactive bacteria have potential applications ranging from sensing to biosynthesis. In order to advance the use of engineered electroactive bacteria, it is important to demonstrate functional expression of electron transfer modules in chassis adapted to operationally relevant conditions, such as non-freshwater environments. Here, we use the Shewanella oneidensis electron transfer pathway to induce current production in a marine bacterium, Marinobacter atlanticus, during biofilm growth in artificial seawater. Genetically encoded sensors optimized for use in E. coli were used to control protein expression in planktonic and biofilm attached cells. Significant current production required addition of menaquinone, which M. atlanticus does not produce, for electron transfer from the inner membrane to the expressed electron transfer pathway. Current through the S. oneidensis pathway in M. atlanticus was observed when inducing molecules were present during biofilm formation. Electron transfer was also reversible, indicating electron transfer into M. atlanticus could be controlled. These results show that an operationally relevant marine bacterium can be genetically engineered for environmental sensing and response using an electrical signal.

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“…The biogas upgrading bioelectrochemical reactor with a 1.3 L working volume was designed as a sleeve reactor (Figure .1) and operated at 37±1℃ following the previous study [11]. The reactor was divided into the anode and cathode chambers by a cylinder (70 mm ×140 mm) made from a cation exchange membrane (240mm × 140mm, CMI-7000, Membranes International Inc., USA).…”

Section: Reactor Setup and Constructionmentioning

confidence: 99%

Electro-fermentation enhances methane production from high-carbon potato starch wastewater

Shang

Guo

2023

IOP Conf. Ser.: Earth Environ. Sci.

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With the development of the potato starch industry, the output of potato starch wastewater has increased rapidly. In terms of the characteristics of potato industry starch wastewater, it was heavily polluted and difficult to degrade, this paper proposes to use the method of electro-fermentation to efficiently treat potato starch wastewater with high COD concentration to realize resource conversion. Under the constant potential control of -1.0 V, the methane yield was significantly increased (maximum 1254 mL/d), and the cumulative methane production increased by 43%. The COD degradation rate reached 90.56%, an increase of 66.53%. Electro-fermentation can solve the bottleneck of anaerobic digestion and is an emerging technology.

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Electro-fermentation: Sustainable bioproductions steered by electricity

Cited by 46 publications

References 205 publications

Anode-assisted electro-fermentation with Bacillus subtilis under oxygen-limited conditions

Anode-assisted electro-fermentation with Bacillus subtilis under oxygen-limited conditions

A living conductive marine biofilm engineered to sense and respond to small molecules

Electro-fermentation enhances methane production from high-carbon potato starch wastewater

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