Electro-biocatalytic production of formate from carbon dioxide using an oxygen-stable whole cell biocatalyst

Hwang, Hea-Ju; Yeon, Young Joo; Lee, Sumi; Choe, Hyunjun; Jang, Min; Cho, Dae Haeng; Park, Sehkyu; Kim, Yong Hwan

doi:10.1016/j.biortech.2015.02.086

Cited by 65 publications

(33 citation statements)

References 12 publications

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“…The reduction of CO 2 to formic acid by using multiwalled carbon nanotubes and cobalt tetra-amino phthalocyanine as the cathode in MEC was reported previously (Zhao et al, 2012). In addition, biocathode immobilized by Methylobacterium extorquens AM1 was also shown to be promise for formic acid production from CO 2 (Hwang et al, 2015). Furthermore, Nevin et al (2010) reported the production of acetate and 2-oxobutyrate from CO 2 in a MEC with Sporomusa Ovata growing on the cathode, and enriched mixed culture grown on the cathode was also feasible for acetate production from CO 2 in MEC (Xiang et al, 2017).…”

Section: Biogas Upgrading Through Microbial Electrochemical Methodsmentioning

confidence: 83%

Biogas upgrading and utilization: Current status and perspectives

Angelidaki

Treu

Tsapekos

et al. 2018

Biotechnology Advances

1,008

560

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Biogas production is an established sustainable process for simultaneous generation of renewable energy and treatment of organic wastes. The increasing interest of utilizing biogas as substitute to natural gas or its exploitation as transport fuel opened new avenues in the development of biogas upgrading techniques. The present work is a critical review that summarizes state-of-the-art technologies for biogas upgrading and enhancement with particular attention to the emerging biological methanation processes. The review includes comprehensive description of the main principles of various biogas upgrading methodologies, scientific and technical outcomes related to their biomethanation efficiency, challenges that have to be addressed for further development and incentives and feasibility of the upgrading concepts.

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Section: Biogas Upgrading Through Microbial Electrochemical Methodsmentioning

confidence: 83%

Biogas upgrading and utilization: Current status and perspectives

Angelidaki

Treu

Tsapekos

et al. 2018

Biotechnology Advances

1,008

560

View full text Add to dashboard Cite

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“…Electrochemical CO 2 reductions were performed as previously reported 19 . The electrochemical reduction system consisted of a copper plate cathode (2 × 1.5 cm), a reference electrode (Ag/AgCl), and a platinum wire as anode electrode.…”

Section: Methodsmentioning

confidence: 99%

“…However, the enzyme primarily responsible for the synthesis of formate from CO 2 remained unknown. Previous studies were focused on the optimization of reaction conditions such as electron mediators, cell mass concentration and pH value 19 .…”

Section: Introductionmentioning

confidence: 99%

Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens

Jang

Jeon

Kim

2018

Sci Rep

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The conversion of carbon dioxide to formate is a fundamental step for building C1 chemical platforms. Methylobacterium extorquens AM1 was reported to show remarkable activity converting carbon dioxide into formate. Formate dehydrogenase 1 from M. extorquens AM1 (MeFDH1) was verified as the key responsible enzyme for the conversion of carbon dioxide to formate in this study. Using a 2% methanol concentration for induction, microbial harboring the recombinant MeFDH1 expressing plasmid produced the highest concentration of formate (26.6 mM within 21 hours) in electrochemical reactor. 60 μM of sodium tungstate in the culture medium was optimal for the expression of recombinant MeFDH1 and production of formate (25.7 mM within 21 hours). The recombinant MeFDH1 expressing cells showed maximum formate productivity of 2.53 mM/g-wet cell/hr, which was 2.5 times greater than that of wild type. Thus, M. extorquens AM1 was successfully engineered by expressing MeFDH1 as recombinant enzyme to elevate the production of formate from CO2 after elucidating key responsible enzyme for the conversion of CO2 to formate.

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“…More recently, by overexpressing suitable recombinant FDHs in E. coli JM109(DE3), high formate yields were obtained from CO 2 hydrogenation, without need for cellular growth on formate for induction [ 129 ]. An alternative whole-cell system was later reported, using an electrochemical cell, where the reducing equivalents are generated by an electrode, rather than H 2 oxidation, as has been done for purified enzymes [ 130 ].…”

Section: Reviewmentioning

confidence: 99%

Biocatalysis for the application of CO₂ as a chemical feedstock

Alissandratos¹,

Easton²

2015

Beilstein J. Org. Chem.

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SummaryBiocatalysts, capable of efficiently transforming CO2 into other more reduced forms of carbon, offer sustainable alternatives to current oxidative technologies that rely on diminishing natural fossil-fuel deposits. Enzymes that catalyse CO2 fixation steps in carbon assimilation pathways are promising catalysts for the sustainable transformation of this safe and renewable feedstock into central metabolites. These may be further converted into a wide range of fuels and commodity chemicals, through the multitude of known enzymatic reactions. The required reducing equivalents for the net carbon reductions may be drawn from solar energy, electricity or chemical oxidation, and delivered in vitro or through cellular mechanisms, while enzyme catalysis lowers the activation barriers of the CO2 transformations to make them more energy efficient. The development of technologies that treat CO2-transforming enzymes and other cellular components as modules that may be assembled into synthetic reaction circuits will facilitate the use of CO2 as a renewable chemical feedstock, greatly enabling a sustainable carbon bio-economy.

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Electro-biocatalytic production of formate from carbon dioxide using an oxygen-stable whole cell biocatalyst

Cited by 65 publications

References 12 publications

Biogas upgrading and utilization: Current status and perspectives

Biogas upgrading and utilization: Current status and perspectives

Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens

Biocatalysis for the application of CO₂ as a chemical feedstock

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Electro-biocatalytic production of formate from carbon dioxide using an oxygen-stable whole cell biocatalyst

Cited by 65 publications

References 12 publications

Biogas upgrading and utilization: Current status and perspectives

Biogas upgrading and utilization: Current status and perspectives

Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens

Biocatalysis for the application of CO2 as a chemical feedstock

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Product

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Biocatalysis for the application of CO₂ as a chemical feedstock