Electro-Acupuncture Inhibits p66Shc-Mediated Oxidative Stress to Facilitate Functional Recovery After Spinal Cord Injury

Cheng, Ming; Wu, Xiaojing; Wang, Fei; Tan, Bo; Hu, Jiang

doi:10.1007/s12031-020-01609-5

Cited by 11 publications

(15 citation statements)

References 36 publications

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“…Fork-head transcription factor (FoxO) is the key effector of JNK-mediated tumor suppression [ 15 ]. Activation of FoxO regulated by JNK enhances the ability of animals to resist oxidative stress and longevity [ 16 , 17 ]. It has been reported [ 18 ] that fork-head box O3a (FoxO3a) is involved in the myocardial injury process caused by IRI, and the activation of the PI3K/Akt pathway can phosphorylate FoxO3a and transfer it from the nucleus to the cytoplasm to participate in reducing the apoptosis of cardiomyocytes.…”

Section: Introductionmentioning

confidence: 99%

Influence of Melatonin on Behavioral and Neurological Function of Rats with Focal Cerebral Ischemia-Reperfusion Injury via the JNK/FoxO3a/Bim Pathway

Chen

Shen

Lai

et al. 2022

Computational and Mathematical Methods in Medicine

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Objective. To investigate the influence of melatonin on behavioral and neurological function of rats with focal cerebral ischemia-reperfusion injury via the JNK/FoxO3a/Bim pathway. Methods. One hundred and twenty healthy male SD rats were randomized into the model group (Model: the middle cerebral artery occlusion (MCAO) model was constructed and received an equal volume of normal saline containing 5% DMSO), sham operation group (Sham: received no treatment except normal feeding), and low, medium, and high dose of melatonin group (L-MT, M-MT, and H-MT intraperitoneally injected 10, 20, and 40 mg/kg melatonin 30 min after IR, respectively), with 24 rats in each group. Following 24 h of reperfusion, the rats in each of the above groups were tested for neurological deficit symptoms and behavioral changes to screen the rats included in the study. HE and TUNEL stainings were performed to observe pathological changes. Levels of oxidative stress-related indexes, inflammatory factor-related indexes, nuclear factor-κB p65 (NF-κB p65), and interferon-γ (IFN-γ) in the rat brain were measured by ELISA. The JNK/FoxO3a/Bim pathway-related proteins as well as Bcl-2, Caspase-3, and Bax were examined using Western blot. Results. Detection of behavioral indicators showed that the MACO model was successfully constructed in rats. L-MT, M-MT, and L-MT groups presented reduced malondialdehyde (MDA), reactive oxygen species (ROS), tumor necrosis factor- (TNF-) α, interleukin- (IL-) 6, IL-1β, IFN-γ, NF-κB p65, and apoptosis compared with the Model group ( P < 0.05 ), and the improvement degree was better in the M-MT group versus the L-HT group. Bcl-2 protein expression in the brain tissue of L-MT, M-MT, and H-MT groups increased significantly, while Bax, Caspase-3, p-JNK, p-FoxO3a, and Bim protein expression declined markedly, versus the Model group ( P < 0.05 ). The changes of indexes were greater in the M-MT group compared with that in the L-MT group. No significant difference was observed in all the above indexes between the M-MT group and the H-MT group ( P > 0.05 ). Conclusions. In the MACO rat model, melatonin can effectively reduce Bax and Caspase-3 levels by modulating the JNK/FoxO3a/Bim pathway, inhibit neuronal apoptosis, and alleviate neurological deficits by reducing the release of proinflammatory mediators, with anti-inflammatory and antioxidant effects. In addition, 20 mg/kg is the optimal melatonin concentration.

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Section: Introductionmentioning

confidence: 99%

Influence of Melatonin on Behavioral and Neurological Function of Rats with Focal Cerebral Ischemia-Reperfusion Injury via the JNK/FoxO3a/Bim Pathway

Chen

Shen

Lai

et al. 2022

Computational and Mathematical Methods in Medicine

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“…Similar to the effect of the reactive oxygen species (ROS) scavenger, acupuncture can inhibit superoxide anion production, decrease JNK/p66Shc-mediated ROS generation, upregulate the apolipoprotein E (ApoE) and nuclear factor E2-related factor 2 (Nrf2)/heme-oxygenase-1 (HO-1) signaling pathways, and reduce the ROS-induced p38MAPK and ERK activation in microglia after SCI ( Choi et al, 2012 ; Dai et al, 2021 ). Notably, the inhibitory effect of electroacupuncture on p38MAPK is significantly enslaved to the acupuncture frequency ( Cheng et al, 2020 ).…”

Section: Mechanism Of Acupuncture Therapy In Spinal Cord Injurymentioning

confidence: 99%

Mechanism Underlying Acupuncture Therapy in Spinal Cord Injury: A Narrative Overview of Preclinical Studies

Jiang¹,

Sun²,

Chen³

2022

Front. Pharmacol.

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Spinal cord injury (SCI) results from various pathogenic factors that destroy the normal structure and function of the spinal cord, subsequently causing sensory, motor, and autonomic nerve dysfunction. SCI is one of the most common causes of disability and death globally. It leads to severe physical and mental injury to patients and causes a substantial economic burden on families and the society. The pathological changes and underlying mechanisms within SCI involve oxidative stress, apoptosis, inflammation, etc. As a traditional therapy, acupuncture has a positive effect promoting the recovery of SCI. Acupuncture-induced neuroprotection includes several mechanisms such as reducing oxidative stress, inhibiting the inflammatory response and neuronal apoptosis, alleviating glial scar formation, promoting neural stem cell differentiation, and improving microcirculation within the injured area. Therefore, the recent studies exploring the mechanism of acupuncture therapy in SCI will help provide a theoretical basis for applying acupuncture and seeking a better treatment target and acupuncture approach for SCI patients.

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“…87 Deng et al 88 and Lv et al 89 found that EA treatment could up-regulate Nrf2 and its downstream antioxidant molecules hemeoxygenase-1 (HO-1) and glutamate-cysteine ligase modifier subunit (GCLM), and reduce the intensity of neuroinflammation. Cheng et al 90 also showed that EA can alleviate the inflammatory response and promote recovery of myeloid functions by inhibiting the oxidative stress response mediated by p66Shc after SCI. He 91 proposed that EA could reduce the level of NO in the brain of VaD rats and increase the content of superoxide dismutase (SOD), so as to mitigate the level of oxidative stress and the impairment of cognitive function.…”

Section: Mechanism Of Ea-induced Attenuation Of Neuroinflammationmentioning

confidence: 99%

Section: Ea Restrains Damage Secondary To Oxidative Stressmentioning

confidence: 99%

Electroacupuncture ameliorates neuroinflammation in animal models

Xin

Wang

2022

Acupunct Med

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Background: Neuroinflammation refers to a wide range of immune responses occurring in the brain or spinal cord. It is closely related to a variety of neurodegenerative diseases, for which it potentially represents a new direction for treatment. Electroacupuncture (EA) is one method of acupuncture treatment, which can be used as an adjuvant therapy for many diseases. This review focuses on molecular mechanisms of EA in the reduction of neuroinflammation, summarizes relevant basic research and outlines future directions for investigation. Findings: A growing body of basic research has shown that EA can ameliorate neuroinflammation centrally (in animal models of ischemic stroke, Alzheimer’s disease, traumatic brain injury, spinal cord injury, Parkinson’s disease and vascular dementia) and peripherally (e.g. after a surgical insult or injection of lipopolysaccharide) and that its effects involve different molecular mechanisms, including activation of the α7 nicotinic acetylcholine receptor signaling pathway and P2 type purinergic receptors, inhibition of nuclear factor κB, and mitigation of damage secondary to oxidative stress and NOD-like receptor protein 3 inflammasome activation. Conclusions: EA is capable of regulating multiple cell signal transduction pathways to alleviate neuroinflammation in animal models. Although the findings of animal studies are encouraging, further prospective clinical trials are needed to verify the efficacy of EA for the treatment of neuroinflammation.

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Electro-Acupuncture Inhibits p66Shc-Mediated Oxidative Stress to Facilitate Functional Recovery After Spinal Cord Injury

Cited by 11 publications

References 36 publications

Influence of Melatonin on Behavioral and Neurological Function of Rats with Focal Cerebral Ischemia-Reperfusion Injury via the JNK/FoxO3a/Bim Pathway

Influence of Melatonin on Behavioral and Neurological Function of Rats with Focal Cerebral Ischemia-Reperfusion Injury via the JNK/FoxO3a/Bim Pathway

Mechanism Underlying Acupuncture Therapy in Spinal Cord Injury: A Narrative Overview of Preclinical Studies

Electroacupuncture ameliorates neuroinflammation in animal models

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