Electrically modulated attachment and detachment of animal cells cultured on an optically transparent patterning electrode

Koyama, Sumihiro

doi:10.1016/j.jbiosc.2010.12.027

Cited by 13 publications

(16 citation statements)

References 21 publications

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“…Either a patterned working ITO electrode or patterned working GZO electrode was constructed by vacuum evaporation of either indium tin oxide (<10 Ω/cm 2 ) or gallium-doped zinc oxide (<10 Ω/cm 2 ) and an insulator of silicon dioxide (SiO 2 ) onto 76 × 26-mm 2 silica glass plates (1 mm thick) (Geomatec Co., Ltd., Yokohama, Japan). Detailed patterning of the reticulated optically transparent working electrode with arrayed square glass regions was described elsewhere (Koyama 2011 ; Koyama et al 2013 ). The 76 × 26-mm 2 and 5-mm-thick silicon rubber plate with a hollow interior measuring 66 × 16 mm 2 was glued to the 76 × 26-mm 2 slide glass by silicon bonding.…”

Section: Methodsmentioning

confidence: 99%

“…Constant and 9-MHz sine-wave potentials were applied to the optically transparent working electrode using the Ag/AgCl reference and the Pt counterelectrode (Koyama 2011 ; Koyama et al 2013 ). The constant potential application was monitored with a potentiostat (PS-14, Toho Technical Research, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…We collected the electrically attached living microorganisms and then detached them from the electrode by applying a high-frequency wave potential (Koyama et al 2013 ). The mechanisms for electrical detachment involve both oscillation of the negative zeta potential-charged microorganisms and insertion of water molecules between the electrode surface and the attached cells resulting from increments in hydrophilicity on the electrode surface (Koyama 2011 ; Koyama et al 2013 ). When we applied the electrical retrieval (ER) method to separate the microorganisms from sediment and soil particles, bacteria belonging to 19 phyla and 23 classes were collected without undesirable high molecular weight contaminants such as humic acids (Koyama et al 2013 ).…”

Section: Introductionmentioning

confidence: 99%

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Electrical Retrieval of Living Microorganisms from Cryopreserved Marine Sponges Using a Potential-Controlled Electrode

et al. 2015

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The purpose of this study was to develop a novel electrical retrieval method (ER method) for living sponge-associated microorganisms from marine sponges frozen at −80 °C. A −0.3-V vs. Ag/AgCl constant potential applied for 2 h at 9 °C induced the attachment of the sponge-associated microorganisms to an indium tin oxide/glass (ITO) or a gallium-doped zinc oxide/glass (GZO) working electrode. The electrically attached microorganisms from homogenized Spirastrella insignis tissues had intact cell membranes and showed intracellular dehydrogenase activity. Dead microorganisms were not attracted to the electrode when the homogenized tissues were autoclaved for 15 min at 121 °C before use. The electrically attached microorganisms included cultivable microorganisms retrieved after detachment from the electrode by application of a 9-MHz sine-wave potential. Using the ER method, we obtained 32 phyla and 72 classes of bacteria and 3 archaea of Crenarchaeota thermoprotei, Marine Group I, and Thaumarchaeota incertae sedis from marine sponges S. insignis and Callyspongia confoederata. Employment of the ER method for extraction and purification of the living microorganisms holds potential of single-cell cultivation for genome, transcriptome, proteome, and metabolome analyses of bioactive compounds producing sponge-associated microorganisms.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrical Retrieval of Living Microorganisms from Cryopreserved Marine Sponges Using a Potential-Controlled Electrode

et al. 2015

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“… 2007 ; Yap and Zhang 2007 ; Ino et al. 2009 ; Robertus, Browne and Feringa 2010 ; Koyama 2011 ). Micropatterning techniques based on animal cell–extracellular matrix interactions utilize the characteristics of cell adhesion activity to extracellular matrix proteins.…”

Section: Introductionmentioning

confidence: 99%

Involvement of flocculin in negative potential-applied ITO electrode adhesion of yeast cells

Koyama

Tsubouchi

Usui

et al. 2015

FEMS Yeast Research

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The purpose of this study was to develop novel methods for attachment and cultivation of specifically positioned single yeast cells on a microelectrode surface with the application of a weak electrical potential. Saccharomyces cerevisiae diploid strains attached to an indium tin oxide/glass (ITO) electrode to which a negative potential between −0.2 and −0.4 V vs. Ag/AgCl was applied, while they did not adhere to a gallium-doped zinc oxide/glass electrode surface. The yeast cells attached to the negative potential-applied ITO electrodes showed normal cell proliferation. We found that the flocculin FLO10 gene-disrupted diploid BY4743 mutant strain (flo10Δ /flo10Δ) almost completely lost the ability to adhere to the negative potential-applied ITO electrode. Our results indicate that the mechanisms of diploid BY4743 S. cerevisiae adhesion involve interaction between the negative potential-applied ITO electrode and the Flo10 protein on the cell wall surface. A combination of micropatterning techniques of living single yeast cell on the ITO electrode and omics technologies holds potential of novel, highly parallelized, microchip-based single-cell analysis that will contribute to new screening concepts and applications.

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“…These six cell micropatterning techniques cannot modulate both the attachment and detachment of animal cells iteratively at the same positions, however. The present work has demonstrated that a weak electrical potential can modulate the attachment and detachment of specifically positioned adhesive animal cells using a patterned indium tin oxide (ITO)/glass electrode culture system [1], (Figure 1). …”

Section: Introductionmentioning

confidence: 99%

Electrically modulated attachment and detachment of animal cells cultured on an optically transparent patterning electrode

Cited by 13 publications

References 21 publications

Electrical Retrieval of Living Microorganisms from Cryopreserved Marine Sponges Using a Potential-Controlled Electrode

Electrical Retrieval of Living Microorganisms from Cryopreserved Marine Sponges Using a Potential-Controlled Electrode

Involvement of flocculin in negative potential-applied ITO electrode adhesion of yeast cells

Electrically modulated attachment and detachment of animal cells cultured on an ITO patterning electrode surface

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