Electrically induced NGF production by astroglial cells

Koyama, Sumihiro; Haruyama, Tetsuya; Kobatake, Eiry; Aizawa, Masuo

doi:10.1038/nbt0297-164

Cited by 54 publications

(32 citation statements)

References 15 publications

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“…1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a neurotoxin which causes PD-like symptoms and is arguably the most commonly used agent to investigate PD pathogenesis. Astrocytes take up MPTP and convert it to MPP+ through the action of monoamine oxidase B (MAO-B) (217, 218). Such discoveries made by using cultured astrocytes have ultimately led to the development of MAO-B inhibitors (selegiline, rasagiline) for the treatment of PD (219).…”

Section: Discoveries Made By Employing Primary Cultures To Understandmentioning

confidence: 99%

Primary Cultures of Astrocytes: Their Value in Understanding Astrocytes in Health and Disease

et al. 2012

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During the past decades of astrocyte research it has become increasingly clear that astrocytes have taken a central position in all central nervous system activities. Much of our new understanding of astrocytes has been derived from studies conducted with primary cultures of astrocytes. Such cultures have been an invaluable tool for studying roles of astrocytes in physiological and pathological states. Many central astrocytic functions in metabolism, amino acid neurotransmission and calcium signaling were discovered using this tissue culture preparation and most of these observations were subsequently found in vivo. Nevertheless, primary cultures of astrocytes are an in vitro model that does not fully mimic the complex events occurring in vivo. Here we present an overview of the numerous contributions generated by the use of primary astrocyte cultures to uncover the diverse functions of astrocytes. Many of these discoveries would not have been possible to achieve without the use of astrocyte cultures. Additionally, we address and discuss the concerns that have been raised regarding the use of primary cultures of astrocytes as an experimental model system.

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Section: Discoveries Made By Employing Primary Cultures To Understandmentioning

confidence: 99%

Primary Cultures of Astrocytes: Their Value in Understanding Astrocytes in Health and Disease

et al. 2012

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“…Additionally, indirect influence on the differentiation of NPCs could potentially be attributed to EF effects on differentiated astrocytes and oligodendrocytes. Astrocytes have been shown to release greater quantities of NGF when stimulated with an EF [64]. Thus, the endogenous EF found in development [1,2] or after injury to the nervous system [11] may synergistically favor the production of new neurons by (1) influencing NPCs via membrane potential depolarization [56,65] and (2) increased neurogenic receptor stimulation (EF-augmented secretion of growth factors by mature nervous system cells).…”

Section: Electric Field Induced Neurogenesismentioning

confidence: 99%

The Influence of Electric Fields on Hippocampal Neural Progenitor Cells

Ariza

Fleury

Tormos

et al. 2010

Stem Cell Rev and Rep

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The differentiation and proliferation of neural stem/progenitor cells (NPCs) depend on various in vivo environmental factors or cues, which may include an endogenous electrical field (EF), as observed during nervous system development and repair. In this study, we investigate the morphologic, phenotypic, and mitotic alterations of adult hippocampal NPCs that occur when exposed to two EFs of estimated endogenous strengths. NPCs treated with a 437 mV/mm direct current (DC) EF aligned perpendicularly to the EF vector and had a greater tendency to differentiate into neurons, but not into oligodendrocytes or astrocytes, compared to controls. Furthermore, NPC process growth was promoted perpendicularly and inhibited anodally in the 437 mV/mm DC EF. Yet fewer cells were observed in the DC EF, which in part was due to a decrease in cell viability. The other EF applied was a 46 mV/mm alternating current (AC) EF. However, the 46 mV/mm AC EF showed no major differences in alignment or differentiation, compared to control conditions. For both EF treatments, the percent of mitotic cells during the last 14 h of the experiment were statistically similar to controls. Reported here, to our knowledge, is the first evidence of adult NPC differentiation affected in an EF in vitro. Further investigation and application of EFs on stem cells is warranted to elucidate the utility of EFs to control phenotypic behavior. With progress, the use of EFs may be engineered to control differentiation and target the growth of transplanted cells in a stem cell-based therapy to treat nervous system disorders.

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“…In addition, calcium is involved in the release of neurotrophic factor from glial cells (Koyama et al, 1997;Trang et al, 2009). Therefore, we further investigated the role of calcium ions in the ES-induced NGF release by using pharmacological interventions.…”

Section: Introductionmentioning

confidence: 99%

Electrical stimulation induces calcium‐dependent release of NGF from cultured Schwann cells

Huang

et al. 2009

Glia

110

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Production of nerve growth factor (NGF) from Schwann cells (SCs) progressively declines in the distal stump, if axonal regeneration is staggered across the suture site after peripheral nerve injuries. This may be an important factor limiting the outcome of nerve injury repair. Thus far, extensive efforts are devoted to modulating NGF production in cultured SCs, but little has been achieved. In the present in vitro study, electrical stimulation (ES) was attempted to stimulate cultured SCs to release NGF. Our data showed that ES was capable of enhancing NGF release from cultured SCs. An electrical field (1 Hz, 5 V/cm) caused a 4.1-fold increase in NGF release from cultured SCs. The ES-induced NGF release is calcium dependent. Depletion of extracellular or/and intracellular calcium partially/ completely abolished the ES-induced NGF release. Further pharmacological interventions showed that ES induces calcium influx through T-type voltage-gated calcium channels and mobilizes calcium from 1, 4, 5-trisphosphate-sensitive stores and caffeine/ryanodine-sensitive stores, both of which contributed to the enhanced NGF release induced by ES. In addition, a calcium-triggered exocytosis mechanism was involved in the ES-induced NGF release from cultured SCs. These findings show the feasibility of using ES in stimulating SCs to release NGF, which holds great potential in promoting nerve regeneration by enhancing survival and outgrowth of damaged nerves, and is of great significance in nerve injury repair and neuronal tissue engineering.

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Electrically induced NGF production by astroglial cells

Cited by 54 publications

References 15 publications

Primary Cultures of Astrocytes: Their Value in Understanding Astrocytes in Health and Disease

Primary Cultures of Astrocytes: Their Value in Understanding Astrocytes in Health and Disease

The Influence of Electric Fields on Hippocampal Neural Progenitor Cells

Electrical stimulation induces calcium‐dependent release of NGF from cultured Schwann cells

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