Electrically induced changes in Ca2+ inHelisoma neurons: Regional and neuron-specific differences and implications for neurite outgrowth

Torreano, Paul J.; Cohan, Christopher S.

doi:10.1002/(sici)1097-4695(199702)32:2<150::aid-neu2>3.0.co;2-7

Cited by 16 publications

(4 citation statements)

References 30 publications

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“…Such a transition from a silent to a firing state constitutes a profound change in the physiological state of a neuron, regardless of whether a neuron is undergoing neurite outgrowth during development or regeneration, or serving as a member of a neuronal circuit in the mature nervous system. For example, neuronal spiking will increase the intracellular Ca 2+ concentration ([Ca 2+ ] i ), which has been shown to have a wide range of effects in both developing and mature nervous systems [35] – [38] . Increases in [Ca 2+ ] i in growth cones from several neuron types have been shown to result in a decrease in neurite outgrowth [39] , filopodial elongation [36] , and growth cone turning [40] .…”

Section: Discussionmentioning

confidence: 99%

Nitric Oxide Regulates Neuronal Activity via Calcium-Activated Potassium Channels

Zhong

Estes²,

Artinian³

et al. 2013

PLoS ONE

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Nitric oxide (NO) is an unconventional membrane-permeable messenger molecule that has been shown to play various roles in the nervous system. How NO modulates ion channels to affect neuronal functions is not well understood. In gastropods, NO has been implicated in regulating the feeding motor program. The buccal motoneuron, B19, of the freshwater pond snail Helisoma trivolvis is active during the hyper-retraction phase of the feeding motor program and is located in the vicinity of NO-producing neurons in the buccal ganglion. Here, we asked whether B19 neurons might serve as direct targets of NO signaling. Previous work established NO as a key regulator of growth cone motility and neuronal excitability in another buccal neuron involved in feeding, the B5 neuron. This raised the question whether NO might modulate the electrical activity and neuronal excitability of B19 neurons as well, and if so whether NO acted on the same or a different set of ion channels in both neurons. To study specific responses of NO on B19 neurons and to eliminate indirect effects contributed by other cells, the majority of experiments were performed on single cultured B19 neurons. Addition of NO donors caused a prolonged depolarization of the membrane potential and an increase in neuronal excitability. The effects of NO could mainly be attributed to the inhibition of two types of calcium-activated potassium channels, apamin-sensitive and iberiotoxin-sensitive potassium channels. NO was found to also cause a depolarization in B19 neurons in situ, but only after NO synthase activity in buccal ganglia had been blocked. The results suggest that NO acts as a critical modulator of neuronal excitability in B19 neurons, and that calcium-activated potassium channels may serve as a common target of NO in neurons.

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Section: Discussionmentioning

confidence: 99%

Nitric Oxide Regulates Neuronal Activity via Calcium-Activated Potassium Channels

Zhong

Estes²,

Artinian³

et al. 2013

PLoS ONE

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“…ES has been reported to affect many cellular processes including gene expression, [ 117 ] Ca 2+ influx, [ 118 ] cytokine expression, [ 119 ] cell migration, [ 120 ] and cell proliferation. [ 121 ] The ability to affect biological processes with electricity is known as electric modulation (EM) and often projected in the form of electric fields (EFs).…”

Section: Electrical Modulation Of Cellsmentioning

confidence: 99%

“…Reversible electroporation is an activity that allows cells to recover after pore formation, while granting entry to [Ca 2+ ] into the cytoplasm. Although this is one school of thought, evidently, Hanna and co-workers' studies [124] have shown a correlation between electropermeabilization and [Ca 2+ ] i , which may lead to the activation of different biological processes [117][118][119][120] including proliferation. [121] Debatably, ES has been hypothesized to activate ion channels to increase [Ca 2+ ] influx.…”

Section: Ef Strength Duration Of Stimulation Frequency and Pulsed Efmentioning

confidence: 99%

Progress on Electro‐Enhancement of Cell Manufacturing

Tian,

Tay

2023

Small Methods

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With the long persistence of complex, chronic diseases in society, there is increasing motivation to develop cells as living medicine to treat diseases ranging from cancer to wounds. While cell therapies can significantly impact healthcare, the shortage of starter cells meant that considerable raw materials must be channeled solely for cell expansion, leading to expensive products with long manufacturing time which can prevent accessibility by patients who either cannot afford the treatment or have highly aggressive diseases and cannot wait that long. Over the last three decades, there has been increasing knowledge on the effects of electrical modulation on proliferation, but to the best of the knowledge, none of these studies went beyond how electro‐control of cell proliferation may be extended to enhance industrial scale cell manufacturing. Here, this review is started by discussing the importance of maximizing cell yield during manufacturing before comparing strategies spanning biomolecular/chemical/physical to modulate cell proliferation. Next, the authors describe how factors governing invasive and non‐invasive electrical stimulation (ES) including capacitive coupling electric field may be modified to boost cell manufacturing. This review concludes by describing what needs to be urgently performed to bridge the gap between academic investigation of ES to industrial applications.

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“…In fact, various electrical stimulation (ES) modalities have been proven effective in regulating cellular activities such as ion transportation and gene expression [ 1 ], production of proteins and intracellular reactive oxygen species [ 2 ], secretion of chemokines and cytokines, neurite growth, and cell migration, etc. [ 3 , 4 , 5 ]. All of these observations suggest that a physiologically relevant endogenous or exogenous ES may serve as an effective tool to affect or regulate cellular and tissue homeostasis [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Electrical Stimulation and Cellular Behaviors in Electric Field in Biomedical Research

2021

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Research on the cellular response to electrical stimulation (ES) and its mechanisms focusing on potential clinic applications has been quietly intensified recently. However, the unconventional nature of this methodology has fertilized a great variety of techniques that make the interpretation and comparison of experimental outcomes complicated. This work reviews more than a hundred publications identified mostly from Medline, categorizes the techniques, and comments on their merits and weaknesses. Electrode-based ES, conductive substrate-mediated ES, and noninvasive stimulation are the three principal categories used in biomedical research and clinic. ES has been found to enhance cell proliferation, growth, migration, and stem cell differentiation, showing an important potential in manipulating cellular activities in both normal and pathological conditions. However, inappropriate parameters or setup can have negative effects. The complexity of the delivered electric signals depends on how they are generated and in what form. It is also difficult to equate one set of parameters with another. Mechanistic studies are rare and badly needed. Even so, ES in combination with advanced materials and nanotechnology is developing a strong footing in biomedical research and regenerative medicine.

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Electrically induced changes in Ca2+ inHelisoma neurons: Regional and neuron-specific differences and implications for neurite outgrowth

Cited by 16 publications

References 30 publications

Nitric Oxide Regulates Neuronal Activity via Calcium-Activated Potassium Channels

Nitric Oxide Regulates Neuronal Activity via Calcium-Activated Potassium Channels

Progress on Electro‐Enhancement of Cell Manufacturing

Electrical Stimulation and Cellular Behaviors in Electric Field in Biomedical Research

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