Electrically driven capillary liquid chromatography at ambient pressure

Seifar, Reza M.; Heemstra, S.; Kok, Wim Th.; Kraak, J.C.; Poppe, H.

doi:10.1002/(sici)1520-667x(1998)10:1<41::aid-mcs6>3.0.co;2-z

Cited by 14 publications

(4 citation statements)

References 17 publications

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“…Electroosmosis may also enhance intraparticle mass transfer due to flow within particles; however, this is still under investigation. [387][388][389] In addition, pressure differentials are not used to drive flow; therefore, it should be possible to use particles smaller than 2 µm without the problems associated with the pressure limits of current instrumentation. Flow rates of 1-3 mm/s are typical and can be easily obtained with 30 kV power supplies.…”

Section: Principles Of Cec For Fast Separationsmentioning

confidence: 99%

“…The plug flow profile associated with EOF decreases band broadening associated with resistance to mass transfer in the mobile phase allowing flat van Deemter curves as demonstrated in several studies. − The flat curves could potentially allow high efficiency at high flow rates and short times. Electroosmosis may also enhance intraparticle mass transfer due to flow within particles; however, this is still under investigation. − In addition, pressure differentials are not used to drive flow; therefore, it should be possible to use particles smaller than 2 μm without the problems associated with the pressure limits of current instrumentation. Flow rates of 1−3 mm/s are typical and can be easily obtained with 30 kV power supplies.…”

Section: Principles Of Cec For Fast Separationsmentioning

confidence: 99%

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Fast Analytical-Scale Separations by Capillary Electrophoresis and Liquid Chromatography

et al. 1999

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Section: Principles Of Cec For Fast Separationsmentioning

confidence: 99%

Section: Principles Of Cec For Fast Separationsmentioning

confidence: 99%

Fast Analytical-Scale Separations by Capillary Electrophoresis and Liquid Chromatography

et al. 1999

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“…The packing material (25 mg) was slurried in 250 mL of acetone, chosen because it gave well-dispersed slurries both with ODS-AQ reversed-phase material and normal-phase silica. Columns were packed at pressures of 500 bar, according to the procedure described elsewhere [7]. Prior to experimental use, columns were conditioned with the running mobile phase using an HPLC pump, Rheos 4000 (Flux Instruments AG, Switzerland), with several column volumes of mobile phase.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of ODS-AQ stationary phase for use in capillary electrochromatography

2001

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The aim of this study was to evaluate the applicability of ODS-AQ packing material as a stationary phase in capillary electrochromatography (CEC). The electroosmotic flow created on an ODS-AQ stationary phase was measured at different mobile phase compositions and at different column temperatures. It was observed that the electroosmotic flow generated in the column increased by 50% when the temperature of the system was raised from 20 degrees C to 60 degrees C, while all other conditions were kept constant. The electroosmotic flow produced by the ODS-AQ stationary phase was found to be comparable to the flow generated in a column packed with Nucleosil bare-silica material. In addition, a set of polar compounds (D-lysergic acid diethylamide derivatives) was utilized to determine the influence of temperature and mobile phase composition on their chromatographic behavior on an ODS-AQ stationary phase in a CEC mode. A linear relationship between the solute retention factor and column temperatures was seen over the temperature range studied (20 degrees C to 60 degrees C). A quadratic function was used to describe the changes in the solute retention factors with variation of acetonitrile concentration in the mobile phase.

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“…The effects of acid modifier, buffer concentration, mobile phase composition, applied voltage, temperature, and pseudostationary phase were thoroughly studied. Stead et al [3] and Seifar et al [4,5] studied the CEC analyses of endogenous steroids such as testosterone and progesterone; Que et al [6] used a macroporous acrylic monolithic stationary phase for isocratic and gradient analysis of endogenous steroids and their derivatives in CEC with laser-induced fluorescence (LIF) and electrospray ionization (ESI) mass spectrometry (MS) detection; Taylor et al [7] demonstrated the application of CEC to the analysis of corticosteroids. Wang et al [8] applied CEC to the analysis of norgestimate and its potential degradation products and reached detection limits as low as 0.01% for degradation impurities.…”

Section: Introductionmentioning

confidence: 99%

Method development for the separation of steroids by capillary electrochromatography

Jiskra

Claessens²,

Cramers

2002

J of Separation Science

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Method development for the separation of steroids by capillary electrochromatographyA rapid capillary electrochromatography (CEC) method was developed to separate five structurally related steroid compounds from the production line of steroid hormones. The separation was performed on a Hypersil C8 MOS and Unimicro C18 stationary phases using acetonitrile (ACN), methanol (MeOH), and tetrahydrofuran (THF) as organic modifiers and tris(hydroxymethyl)aminomethane (Tris) as buffer additive. The Hypersil C8 MOS stationary phase performed best together with ACN as organic modifier and Tris buffer. The method was extensively tested for ruggedness with respect to sensitivity to temperature, ACN composition, pH change, concentration of Tris buffer, injected plug length, and run-to-run and day-to-day repeatability. The minimal detectable concentration and amount were investigated for quantification purposes. The developed CEC method was shown to be fast, rugged, and well suited for quantification of the steroids under study.

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Electrically driven capillary liquid chromatography at ambient pressure

Cited by 14 publications

References 17 publications

Fast Analytical-Scale Separations by Capillary Electrophoresis and Liquid Chromatography

Fast Analytical-Scale Separations by Capillary Electrophoresis and Liquid Chromatography

Evaluation of ODS-AQ stationary phase for use in capillary electrochromatography

Method development for the separation of steroids by capillary electrochromatography

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