Electrically conductive nanomaterials for cardiac tissue engineering

Ashtari, Khadijeh; Nazari, Hojjatollah; Ko, Hyojin; Tebon, Peyton; Akhshik, Masoud; Akbari, Mohsen; Alhosseini, Sanaz Naghavi; Mozafari, Masoud; Mehravi, Bita; Soleimani, Masoud; Ardehali, Reza; Warkiani, Majid Ebrahimi; Ahadian, Samad; Khademhosseini, Ali

doi:10.1016/j.addr.2019.06.001

Cited by 147 publications

(109 citation statements)

References 170 publications

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“…The nanotopography and electrical conductivity of scaffolds plays an important role in improving cell‐scaffold interactions in heart tissue engineering . Different kinds of and nanoparticles or nanostructures have been used for fabrication of nano‐enabled electrically conductive scaffolds for cardiac tissue engineering . A part of these nanostructures are 2D nanosheets, super‐molecules which consist of a layer atoms, such as graphene or MoS2.…”

Section: Discussionmentioning

confidence: 94%

Incorporation of two‐dimensional nanomaterials into silk fibroin nanofibers for cardiac tissue engineering

Nazari

Heirani‐Tabasi

Hajiabbas

et al. 2019

Polymers for Advanced Techs

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Mimicking the extracellular matrix to have a similar nanofibrous structure regarding electrical conductivity and mechanical properties would be highly beneficial for cardiac tissue engineering. The molybdenum disulfide, MoS 2 , and reduced graphene oxide, rGO, nanosheets are two-dimensional nanomaterials which can be considered as great candidates for enhancing the electrical and mechanical properties of biological scaffolds for cardiac tissue engineering applications. In this study, MoS 2 and rGO nanosheets were synthesized and incorporated into silk fibroin nanofibers, SF, via electrospinning method. Then, the human iPSCs transfected with TBX-18 gene, TBX18-hiPSCs, were seeded on these scaffolds for in vitro studies. The MoS 2 and rGO nanosheets were studied by Raman spectroscopy. After incorporation of the nanosheets into SF nanofibers, the associated characterizations were carried out including scanning electron microscopy, transmission electron microscopy, water contact angle, and mechanical test. Furthermore, SF, SF/MoS 2 , and SF/rGO scaffolds were used for in vitro studies. Herein, the scaffolds exhibited acceptable biocompatibility and considerable attachment to TBX18-hiPSCs confirmed by 3-(4, 5dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, MTT, assay, and scanning electron microscopy. Also, the real-time PCR and immunostaining studies confirmed the maturity and upregulation of cardiac functional genes, including GATA-4, c-TnT, and α-MHC in the SF/MoS 2 and SF/rGO scaffolds compared with the bare SF one.Therefore, the reinforcement of these SF-based scaffolds with MoS 2 and rGO endues them as a suitable candidate for cardiac tissue engineering.

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Section: Discussionmentioning

confidence: 94%

Incorporation of two‐dimensional nanomaterials into silk fibroin nanofibers for cardiac tissue engineering

Nazari

Heirani‐Tabasi

Hajiabbas

et al. 2019

Polymers for Advanced Techs

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“…Cardiac tissue engineering favors the use of materials that provide similar physicochemical properties to native ECM and are more biocompatible and physiologically relevant to native tissues (Ashtari et al, 2019). Among the experimental materials, natural biomaterials appeared to better satisfy these desires compared to synthetics, particularly naturally derived dECMs (Badylak, Freytes, & Gilbert, 2009; Traverse et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Decellularized muscle‐derived hydrogels support in vitro cardiac microtissue fabrication

et al. 2020

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Cardiovascular research has considerably benefited from in vitro models of cardiac tissue. Two important elements of these constructs, cardiac cells and the extracellular matrix (ECM), play essential roles that mimic the structural and functional aspects of myocardium. Here, we compared decellularized ECM from cardiac muscle (D-CM), skeletal muscle (D-SM), aorta (D-Ao), liver (D-Liv), small intestine submucosa (D-SIS), and human umbilical cord (D-hUC) in terms of their biocompatibility and potential for differentiation of human embryonic stem cell-derived cardiac progenitor cells (hESCderived CPCs) to cardiovascular lineage cells. The decellularization procedure successfully removed resident cells of the tissues but preserved ECM components such as laminin and fibronectin, which was identified by histological studies of decellularized tissue (D-tissues) and immunostaining. Encapsulation of hESC-derived CPCs and human umbilical vein endothelial cells within hydrogels that were obtained from all decellularized tissues did not induce cytotoxicity after 10 days of culture. Upregulation of cardiac specific genes, cTNT and αMHC, as well as the presence of cTNT + cardiomyocytes were also observed in CPCs cultured on D-CM, D-SM, D-Liv, and D-SIS, which showed their support for cardiogenic differentiation. However, D-CM provided substantially higher expression of cardiac markers compared to the other D-tissues. The endothelial and smooth muscle specific genes, CD31 and PDGFRα, were upregulated in cells cultured on D-Ao and D-hUC, which reflected their support for vascular lineage cell differentiation. In conclusion, it might be imperative to use decellularized tissue of muscle origins in combination with naturally derived vascular tissues to generate in vitro vascularized human cardiac microtissues. K E Y W O R D S cardiac progenitor cells, extracellular matrix, hydrogel, tissue engineering 1 | INTRODUCTION Regenerative therapy for cardiac ischemic diseases is a developing scientific field that has attracted considerable attention. In particular, tissue engineering has emerged as a very promising supplement to favorable cell therapy approaches (Saludas, Pascual-Gil, Prosper, Garbayo, & Blanco-Prieto, 2017). Acellular therapies based on tissue engineering have shown substantial outcomes (Liberski, Latif, Raynaud, Bollensdorff, & Yacoub, 2016; Traverse et al., 2019). Among proposed biomaterials, the extracellular matrix (ECM) provides cells with environments that have the highest similarity to their native niche (Yi, Ding, Gong, & Gu, 2017), resulting in similar biochemical profiles and growth factor reserves that help to mimic the tissue microenvironment and support function (Freedman et al., 2015;

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“…This has been the case of load‐bearing and complex electroactive tissues (e.g., cardiac, nerve, and skeletal muscle) . In this regard, several electrically conductive nanomaterials, such as graphene, carbon nanotubes, and silicon/gold nanowires, provided promising outcomes in cardiac tissue engineering . By impregnating gold nanowires in alginate scaffolds researchers were able to enhance the electrical interconnectivity of engineered cardiac 3D microtissues and improve cardiomyocytes phenotype, expression of contractile function markers and ability to generate synchronous contractions .…”

Section: Cell–biomaterials Assembliesmentioning

confidence: 99%

Advanced Bottom‐Up Engineering of Living Architectures

et al. 2019

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Bottom‐up tissue engineering is a promising approach for designing modular biomimetic structures that aim to recapitulate the intricate hierarchy and biofunctionality of native human tissues. In recent years, this field has seen exciting progress driven by an increasing knowledge of biological systems and their rational deconstruction into key core components. Relevant advances in the bottom‐up assembly of unitary living blocks toward the creation of higher order bioarchitectures based on multicellular‐rich structures or multicomponent cell–biomaterial synergies are described. An up‐to‐date critical overview of long‐term existing and rapidly emerging technologies for integrative bottom‐up tissue engineering is provided, including discussion of their practical challenges and required advances. It is envisioned that a combination of cell–biomaterial constructs with bioadaptable features and biospecific 3D designs will contribute to the development of more robust and functional humanized tissues for therapies and disease models, as well as tools for fundamental biological studies.

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Electrically conductive nanomaterials for cardiac tissue engineering

Cited by 147 publications

References 170 publications

Incorporation of two‐dimensional nanomaterials into silk fibroin nanofibers for cardiac tissue engineering

Incorporation of two‐dimensional nanomaterials into silk fibroin nanofibers for cardiac tissue engineering

Decellularized muscle‐derived hydrogels support in vitro cardiac microtissue fabrication

Advanced Bottom‐Up Engineering of Living Architectures

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