Electrically Conductive Gold-Coated Collagen Nanofibers for Placental-Derived Mesenchymal Stem Cells Enhanced Differentiation and Proliferation

Abstract

Gold-coated collagen nanofibers (GCNFs) were produced by a single-step reduction process and used for the growth and differentiation of human adult stem cells. The nanomaterials were characterized by a number of analytical techniques including electron microscopy and spectroscopy. They were found to be biocompatible and to improve the myocardial and neuronal differentiation process of the mesenchymal stem cells isolated from the placental chorionic component. The expression of specific differentiation markers (atrium, natriuretic peptide, actin F and actin monomer, glial fibrilary acidic protein, and neurofilaments) was investigated by immunocytochemistry.

Reversing chemoresistance of malignant glioma stem cells using gold nanoparticles

“…Generally, gold nanoparticles have been reported to have good biocompatibility with nonstem cells, but with effects dependent upon the particular cell line used in the experiment. [32][33][34] Moreover, the surface chemistry, 35 size, 35 and concentration have been shown to affect the internalization mechanism for such nanomaterials. 36 The surface modifiers used to stabilize gold nanoparticles include a range of anionic, cationic, and neutral groups, such as citrate, amine, and glucose.…”

Nano-Engineered Biomaterials for Tissue Regeneration: What Has Been Achieved So Far?

“…The incorporation of neonatal cardiac cells into these AuNP-conjugated scaffolds reduced the threshold required for contraction compared to the biomaterial alone, which was accompanied by greater gap junction protein expression, striation formation similar to cardiac muscle, and velocity of calcium of transients in response to electrical stimulation (Shevach et al, 2014). AuNPs have also been incorporated into thermo-responsive hydrogels of collagen nanofibers (Orza et al, 2011) and chitosan polymer (Baei et al, FiGURe 2 | Schematic illustrating the properties of nanoparticles that can enhance the design and function of tissue engineering constructs, either injectable materials or cardiac patches, for cardiac regeneration. (i) metal oxide nanoparticles, including reduced graphene oxide (RGO) flakes have antioxtdant properties to protect stem cells [e.g., mesenchymal stem cells (MSCs)] delivered to the infarcted myocardium from reactive oxygen species (ROS).…”

Influence of nanomaterials on stem cell differentiation: designing an appropriate nanobiointerface

“…12 Researchers have focused on application of nanomaterials in the biomedical field because of the fact that provision of an appropriate nanobiointerface can secure the control of cellular behavior, and, therefore, optimal tissue regeneration. [13][14][15][16] Moreover, research activity in this field has been fuelled by recent advances in nanomaterial preparation, increasing awareness on the part of materials science and tissue engineering researchers regarding the potential role of stem cells in regenerative medicine, and advances in stem cell biology. Most of the research has focused on development of novel nanoparticles or nanotubes for stem cell imaging 17 and for potential delivery of chemotherapeutic agents to tumors, respectively.…”