Electrically charged polymeric substrates enhance nerve fibre outgrowth In vitro

Valentini, Robert F.; Vargo, Terrence G.; Gardella, Joseph A.; Aebischer, Patrick

doi:10.1016/0142-9612(92)90069-z

Cited by 186 publications

(106 citation statements)

References 23 publications

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“…Extracellular electric fields (0.1-10 V/cm) applied in solution reversibly influenced the direction of neurite growth and increased the neurite initiation and length in Xenopus (Li and Hoffman-Kim, 2008). Applied electrical fields have been shown to influence the rate and orientation of neurite outgrowth from cultured neurons in vitro (Valentini et al, 1992). For example, applied electric fields influenced the extension and direction of neurite outgrowth from neurons cultured in vitro and pulsed electromagnetic fields stimulated sciatic nerve regeneration in vivo .…”

Section: Electrical Stimulation and Its Effectsmentioning

confidence: 99%

“…Piezoelectric polymeric materials generate transient surface charges by tiny mechanical deformations of the material under minute mechanical strain and do not require additional energy sources or electrodes (Schmidt et al, 1997;Valentini et al, 1992). Poly(vinylidenefluoride) (PVDF) is a synthetic, semicrystalline polymer with piezoelectric properties that generate transient surface charges due to their unique molecular structure (Valentini et al, 1992).…”

Section: Piezoelectric Polymeric Materialsmentioning

confidence: 99%

“…Enhanced neurite outgrowth has been attributed to the presence of their surface charges and transient charge generation (Schmidt et al, 1997;Aebischer et al, 1987). The use of intrinsically charged piezoelectric polymers as tissue culture substrates provide a means of exposing cells directly to local time-varying charges, such as those produced by elements of ECM (Valentini et al, 1992).…”

Section: Piezoelectric Polymeric Materialsmentioning

confidence: 99%

“…The inherent nature of neurons is to transmit electrochemical signals throughout the nervous system and, as a result, they are highly influenced by electrical stimuli (Yu et al, 2008b). Previous studies have shown that electrical stimulation is an effective cue in stimulating either the proliferation or differentiation of various cell types McCaig and Zhao, 1997;Sun et al, 2006;Ciombor and Aaron, 1993;Aaron and Ciombor, 1993;Goldman and Pollack, 1996;Dust and Bawornluck, 2006;Shi et al, 2008;Zhao et al,1996Zhao et al, , 1999Yaoita et al,1990;Guimarda et al, 2007;Rivers et al, 2002;Jeong et al, 2008;Whitehead et al, 2007;Ateh et al, 2006;Schmidt et al, 2003;Valentini et al, 1992). In this review we discuss the electrical properties of nerve cells, the most commonly utilized conductive polymers, namely polypyrrole (PPy) and polyaniline (PANI), the principle of electrical stimulation and the application of electrical stimulation through conductive scaffolds to nerve cells.…”

Section: Introductionmentioning

confidence: 99%

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Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Ghasemi‐Mobarakeh¹,

Prabhakaran²,

Morshed³

et al. 2011

J Tissue Eng Regen Med

603

413

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Among the numerous attempts to integrate tissue engineering concepts into strategies to repair nearly all parts of the body, neuronal repair stands out. This is partially due to the complexity of the nervous anatomical system, its functioning and the inefficiency of conventional repair approaches, which are based on single components of either biomaterials or cells alone. Electrical stimulation has been shown to enhance the nerve regeneration process and this consequently makes the use of electrically conductive polymers very attractive for the construction of scaffolds for nerve tissue engineering. In this review, by taking into consideration the electrical properties of nerve cells and the effect of electrical stimulation on nerve cells, we discuss the most commonly utilized conductive polymers, polypyrrole (PPy) and polyaniline (PANI), along with their design and modifications, thus making them suitable scaffolds for nerve tissue engineering. Other electrospun, composite, conductive scaffolds, such as PANI/gelatin and PPy/poly(ε-caprolactone), with or without electrical stimulation, are also discussed. Different procedures of electrical stimulation which have been used in tissue engineering, with examples on their specific applications in tissue engineering, are also discussed.

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Section: Electrical Stimulation and Its Effectsmentioning

confidence: 99%

Section: Piezoelectric Polymeric Materialsmentioning

confidence: 99%

Section: Piezoelectric Polymeric Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Ghasemi‐Mobarakeh¹,

Prabhakaran²,

Morshed³

et al. 2011

J Tissue Eng Regen Med

603

413

View full text Add to dashboard Cite

show abstract

“…According with electrical stimulation here exposed, to improve neural growth few studies have investigated neural tissue engineering applications of piezoelectric materials. A study regarding to piezoelectric properties of polyvinylidene fluoride (PVDF) polymer (70) where mechanical stress is caused by vibrations was realized. The piezoelectric response enhanced neural cell (Nb2a) differentiation and neurite outgrowth compared to non-piezoelectric controls.…”

Section: Canillas B Moreno-burriel E Chinarromentioning

confidence: 99%

Materials directed to implants for repairing Central Nervous System

Canillas¹,

Moreno²,

Chinarro³

2014

Bol. Soc. Esp. Ceram. Vidr.

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Human monocyte morphology is affected by local substrate charge heterogeneity

Kapur

Lilien

Picciolo

et al. 1996

J. Biomed. Mater. Res.

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Cells are sensitive to topological, chemical, and electrical properties of substrates on which they are grown. However, most studies of cell-surface interactions have neglected electrical effects or confounded them with other substrate properties. The use of nanofabrication technology has made it possible to fabricate optically transparent surfaces with controlled chemistry and topology, and with active, controllable surface charge density in domains as small as 1-4 microns. Human monocytes incubated on polystyrene with 3.3 microns-wide strip domains, alternately charged so as to maintain overall charge neutrality, show significant charge density and time-dependent increases (greater than twofold) in cell area and cell perimeter after challenge with a phagocytic trigger (human IgG opsonized zymosan particles). Additional utlrastructural studies on silicon dioxide substrates show charge-density-dependent qualitative morphological differences. These studies clearly demonstrate that human monocytes respond in vitro to local surface-charge heterogeneity in the absence of substrate topology and compositional variation.

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Electrically charged polymeric substrates enhance nerve fibre outgrowth In vitro

Cited by 186 publications

References 23 publications

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Materials directed to implants for repairing Central Nervous System

Human monocyte morphology is affected by local substrate charge heterogeneity

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