Electrical Stimulation, Recording and Impedance-Based Real-Time Position Detection of Cultured Neurons Using Thin-Film-Transistor Array

Shaik, Faruk Azam; Cathcart, Grant A.; Ihida, Satoshi; Ikeuchi, Yoshiho; Tixier‐Mita, Agnès; Toshiyoshi, Hiroshi

doi:10.1109/jmems.2018.2810285

Cited by 6 publications

(3 citation statements)

References 31 publications

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“…Recently, the research group of the authors has got interest in using the TFT technology for biological applications, such as electrophysiology, cells culture monitoring by impedance sensing and bio-sensing [3][4][5]. Actually, when compared to commercial MEAs with 64 electrodes per chip in general, TFT-µEAs are advantageous in higher electrode density with about 1.2 × 10 4 electrodes cm -2 (50-100 µm pitches and ∼5 µm spacing between neighboring electrodes) to avoid misaligning with cells, size of which is about 10-20 µm in diameter [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, Bakkum et al have conducted a two-dimensional mapping analysis for cell viability and function using human-derived cells, such as human hepatocarcinoma cell line Hep G2, by electrical impedance variation simultaneously with microscopic observation of fluorescent imaging on the transparent TFT-µEAs [2]. Furthermore, real-time extracellular potential measurement for cardiomyocytes and neural cells was also performed with similar devices toward the application to electrophysiological research [4,5]. It offers an alternative to MEA and CMOS for characterizing two-dimensional analyses of cardiomyocytes and neural cells on a large surface, with using high resolution and a dimension of pixels is as small as few cells.…”

Section: Introductionmentioning

confidence: 99%

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Toward the development of a label-free multiple immunosensor based on thin film transistor microelectrode arrays

Zhu

Cathcart

Ihida

et al. 2021

J. Micromech. Microeng.

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A label-free immunosensor using thin film transistor microelectrode arrays (TFT-µEAs) derived from flat-panel technology has been developed for the first time. The TFT-µEAs used here incorporate a large sensing area of 15 mm × 15 mm with a 150 × 150 array of transparent indium tin oxide (ITO) electrodes placed at a 100 μm pixel pitch. Anti-human serum albumin (HSA) IgG was characterized to be immobilized on functionalized ITO electrodes. HSA, which is one of the indexes for liver functions, was successfully detected by recording the change of real-time current based on the change in the electric double layer capacitance before and after conjugation of HSA with anti-HSA IgG. Ionic strength and pH value of HSA solution were optimized. In 0.01 mM phosphate buffered saline with pH 6.0, linear range of HSA was determined as 0.3–300 pM. The present TFT-µEAs are applicable to a label-free multiple immunosensing by patterning antibodies on its surface.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Toward the development of a label-free multiple immunosensor based on thin film transistor microelectrode arrays

Zhu

Cathcart

Ihida

et al. 2021

J. Micromech. Microeng.

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“…In addition, these microelectrodes can also be used as sensors like impedance sensors. Actually, the authors have already shown the possibility to perform impedance sensing on cells, to stimulate neurons, and register their activity, or to perform electrowetting droplet motion for cells patterning [27][28][29]. As a consequence, a versatile device is proposed, with which cells patterning can be performed by DEP, creating whatever complex the pattern has to be, and with sensing possibility to monitor cells positioning or cells culture evolution.…”

Section: Introductionmentioning

confidence: 99%

2D Dielectrophoresis using an active matrix array made by thin‐film‐transistor technology

Tixier‐Mita

Ihida

Blanchard³

et al. 2019

IEEJ Transactions Elec Engng

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New types of devices made using thin‐film‐transistor (TFT) technology were used in this article for dielectrophoresis purpose with microbeads, yeast cells, and HepG2 cells. That technology allows obtaining centimeter size transparent substrates covered with a large and dense array of 100 µm microelectrodes individually controllable. The purpose of these devices is the development of platforms for various applications on cells and cells culture, like manipulation, sensing, monitoring, or stimulation on the same device. Dielectrophoresis is one of the applications and is a key target for precise patterning and 2D displacement of cells on the device. In this article, 2D results of dielectrophoresis with microbeads and yeast cells and device programming are described. Then, application to mammal HepG2 liver cells shows the possibility of cells patterning with such devices. © 2019 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.

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Single Cell Electrophysiology

Shaik¹,

Ihida²,

Tixier‐Mita³

et al. 2020

Handbook of Single Cell Technologies

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Electrical Stimulation, Recording and Impedance-Based Real-Time Position Detection of Cultured Neurons Using Thin-Film-Transistor Array

Cited by 6 publications

References 31 publications

Toward the development of a label-free multiple immunosensor based on thin film transistor microelectrode arrays

Toward the development of a label-free multiple immunosensor based on thin film transistor microelectrode arrays

2D Dielectrophoresis using an active matrix array made by thin‐film‐transistor technology

Single Cell Electrophysiology

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