Electrical stimulation of human mesenchymal stem cells on biomineralized conducting polymers enhances their differentiation towards osteogenic outcomes

Hardy, John G.; Sukhavasi, Rushi C.; Aguilar, David; Villancio-Wolter, Maria K.; Mouser, David J.; Geissler, Sydney A.; Nguy, Lindsey; Chow, Jacqueline K.; Kaplan, David L.; Schmidt, Christine E.

doi:10.1039/c5tb00714c

Cited by 38 publications

(24 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As an alternative, MSCs with self-renewing abilities can differentiate into chondrocytes and offer a reliable resource for ES-based therapies for damaged cartilage defects. However, although recent studies have reported the effects of ES on proliferation and differentiation of MSCs3637, it remains unclear whether ES induces chondrogenic differentiation of MSCs.…”

mentioning

confidence: 99%

Electrical stimulation drives chondrogenesis of mesenchymal stem cells in the absence of exogenous growth factors

Kwon

Lee

Chun

2016

Sci Rep

View full text Add to dashboard Cite

Electrical stimulation (ES) is known to guide the development and regeneration of many tissues. However, although preclinical and clinical studies have demonstrated superior effects of ES on cartilage repair, the effects of ES on chondrogenesis remain elusive. Since mesenchyme stem cells (MSCs) have high therapeutic potential for cartilage regeneration, we investigated the actions of ES during chondrogenesis of MSCs. Herein, we demonstrate for the first time that ES enhances expression levels of chondrogenic markers, such as type II collagen, aggrecan, and Sox9, and decreases type I collagen levels, thereby inducing differentiation of MSCs into hyaline chondrogenic cells without the addition of exogenous growth factors. ES also induced MSC condensation and subsequent chondrogenesis by driving Ca2+/ATP oscillations, which are known to be essential for prechondrogenic condensation. In subsequent experiments, the effects of ES on ATP oscillations and chondrogenesis were dependent on extracellular ATP signaling via P2X4 receptors, and ES induced significant increases in TGF-β1 and BMP2 expression. However, the inhibition of TGF-β signaling blocked ES-driven condensation, whereas the inhibition of BMP signaling did not, indicating that TGF-β signaling but not BMP signaling mediates ES-driven condensation. These findings may contribute to the development of electrotherapeutic strategies for cartilage repair using MSCs.

show abstract

mentioning

confidence: 99%

Electrical stimulation drives chondrogenesis of mesenchymal stem cells in the absence of exogenous growth factors

Kwon

Lee

Chun

2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…8 In this research, we attempted to evaluate their possible functional synergism for bone tissue engineering. 8 In this research, we attempted to evaluate their possible functional synergism for bone tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, direct stimulation, capacitive-coupling, and inductive-coupling methods were utilized to deliver electrical stimulus with dissimilar effects on osteoblasts and mesenchymal stem cells. [7][8][9][10][11][12][13] Among conductive polymers, polypyrrole, polyaniline, and polythiophenes have been extensively utilized in biomedical engineering as biosensors, neural probes, tissue engineering scaffolds, drug delivery carriers, and so on. On the other hand, inductive-coupling inhibited cell growth and enhanced mineralization and expression of BMP-2 and transforming growth factor beta 1 (TGFb-1).…”

Section: Introductionmentioning

confidence: 99%

Sequential application of mineralized electroconductive scaffold and electrical stimulation for efficient osteogenesis

Oftadeh

Bakhshandeh

Dehghan

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

Osteogenic differentiation is enhanced by many inductive factors including biochemical agents, biomechanical stresses, and electrical stimulation. Regularly studies have focused on one factor at a time, while synergies can promote more effective and functional osteogenesis. Herein, for the first time, functional synergism between application of electrical stimulation and HA nanoparticles was evaluated in osteogenic differentiation. Prepared electrospun biocompatible conductive scaffold by amalgamating chitosan, aniline-pentamer, and hydroxyapatite incorporation was seeded by human bone-marrow-derived mesenchymal stem cells. The cells seeded on the scaffolds with and without hydroxyapatite were exposed to electrical stimulation and subsequently, osteogenic molecular markers and related signaling pathways were investigated. In general, all investigated osteogenic markers (osteocalcin, alkaline phosphatase, osteonectin, and Runx2) were upregulated transcriptionally in the cells seeded on the chitosan-embedded scaffolds. Separate utilization of electrical stimulation or hydroxyapatite-enhanced osteogenesis, while the cells exposed to both stimulators simultaneously, expressed higher levels of some of osteogenic genes significantly. Considering the functions and the positions of the markers in osteogenic signaling pathways, it can be concluded that HA might cooperate in the allocation of stem cells to osteoprogenitors in the early phase of osteogenesis while electrical stimulation helps committed cells with maturation and acquiring functional phenotypes. Altogether investigation of the synergism between different stimulators and exploiting the interactions in an optimized manner could lead to more efficient osteogenesis protocol for effective bone regeneration and tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1200-1210, 2018.

show abstract

“…The beneficial effect of cell interaction with an electrically active substrate based on CPs has been well documented on cell growth, attachment and proliferation, as well as control of cell migration and protein conformation . More recently CPs are being used to manipulate the properties of stem cells through electrical or photostimulation .…”

Section: Conjugated Polymers Tested In Vivomentioning

confidence: 99%

Conjugated Polymers in Bioelectronics: Addressing the Interface Challenge

Fidanovski

Mawad

2019

Adv Healthcare Materials

View full text Add to dashboard Cite

Conjugated polymers are the material of choice for organic bioelectronic interfaces as they combine mechanical flexibility with electric and ionic conductivity. Their attractive properties are largely demonstrated in vitro, while the in vivo applications are limited to the coating of inorganic electrodes, where they are used to improve the intimate electronic contact between the device and the tissue. However, there has not been a commensurate rise in the in vivo applications of entirely organic implantable electronic devices based on conjugated polymers. To date, there is no comprehensive understanding of how these devices will interface with real biological systems. With the push toward increasingly thinner and more flexible next generation medical implants, this limitation remains a major detractor in the translation of conjugated polymers toward biological applications. This research news article examines the few reported in vivo studies and attempts to establish why there is such a dearth in the literature.

show abstract

Electrical stimulation of human mesenchymal stem cells on biomineralized conducting polymers enhances their differentiation towards osteogenic outcomes

Abstract: Tissue scaffolds allowing the behaviour of the cells that reside on them to be controlled are of particular interest for tissue engineering.

Cited by 38 publications

References 85 publications

Electrical stimulation drives chondrogenesis of mesenchymal stem cells in the absence of exogenous growth factors

Electrical stimulation drives chondrogenesis of mesenchymal stem cells in the absence of exogenous growth factors

Sequential application of mineralized electroconductive scaffold and electrical stimulation for efficient osteogenesis

Conjugated Polymers in Bioelectronics: Addressing the Interface Challenge

Contact Info

Product

Resources

About