Electrical Stimulation Elicits Neural Stem Cells Activation: New Perspectives in CNS Repair

Huang, Yanhua H.; Li, YeE; Chen, Jian; Zhou, Hongxing; Tan, Suxu

doi:10.3389/fnhum.2015.00586

Cited by 54 publications

(61 citation statements)

References 79 publications

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“…Pharmaceutical medicines are not efficient in spinal cord injury and traumatic brain injury. Tissue engineering is an almost new procedure to restore and regenerate the functions of damaged cells or tissue . Stem cells and scaffolds are 2 main factors which play an exceptionally significant role in tissue engineering .…”

Section: Discussionmentioning

confidence: 99%

“…Conductive scaffolds have the capability to transmit electrical current to the cell‐seeded scaffold to regulate the biophysical properties of cells; increased proliferation, stem cell differentiation, and neurite extension . Electrical stimulation via conductive scaffold, as a new approach, has some effects on axonal regeneration, cell migration, and neural differentiation of cells …”

Section: Introductionmentioning

confidence: 99%

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Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

et al. 2019

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An electrical stimulus is a new approach to neural differentiation of stem cells. In this work, the neural differentiation of conjunctiva mesenchymal stem cells (CJMSCs) on a new 3D conductive fibrous scaffold of silk fibroin (SF) and reduced graphene oxide (rGo) were examined. rGo (3.5% w/w) was dispersed in SF‐acid formic solution (10% w/v) and conductive nanofibrous scaffold was fabricated using the electrospinning method. SEM and TEM microscopies were used for fibrous scaffold characterization. CJMSCs were cultured on the scaffold and 2 electrical impulse models (Current 1:115 V/m, 100‐Hz frequency and current 2:115 v/m voltages, 0.1‐Hz frequency) were applied for 7 days. Also, the effect of the fibrous scaffold and electrical impulses on cell viability and neural gene expression were examined using MTT assay and qPCR analysis. Fibrous scaffold with the 220 ± 20 nm diameter and good dispersion of graphene nanosheets at the surface of nanofibers were fabricated. The MTT result showed the viability of cells on the scaffold, with current 2 lower than current 1. qPCR analysis confirmed that the expression of β‐tubulin (2.4‐fold P ≤ 0.026), MAP‐2 (1.48‐fold; P ≤ 0.03), and nestin (1.5‐fold; P ≤ 0.03) genes were higher in CJMSCs on conductive scaffold with 100‐Hz frequency compared to 0.1‐Hz frequency. Collectively, we proposed that SF‐rGo fibrous scaffolds, as a new conductive fibrous scaffold with electrical stimulation are good strategies for neural differentiation of stem cells and the type of electrical pulses has an influence on neural differentiation and proliferation of CJMSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

et al. 2019

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“…One strategy to overcome these impairments ideally would be to produce biomaterials that provide a favorable matrix for neuroregeneration. [ 41,42 ] Being the most abundant cell type in the CNS, astrocytes are indispensable for the brain. They are involved in synaptic formation and pruning, functioning of synapses, and establishment of neuronal circuitries.…”

Section: Cellular Attachment and Proliferation Of Ccf-sttg1 And Sh-mentioning

confidence: 99%

Poly(3,4‐ethylenedioxythiophene):GlycosAminoGlycan Aqueous Dispersions: Toward Electrically Conductive Bioactive Materials for Neural Interfaces

Mantione

Agua

Schaafsma³

et al. 2016

Macromolecular Bioscience

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GAGs coatings are performed using SH-SY5Y and CCF-STTG1 cell lines and with ATP and Ca(2+) . Results show full biocompatibility and a pronounced anti-inflammatory effect. This last characteristic becomes crucial if implanted in the body. These materials can be used for in vivo applications, as transistor or electrode for electrical recording and for all the possible situations when there is contact between electronic circuits and living tissues.

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“…The increase of neuroblast migration has potential significance for NSC therapy in neurodegenerative diseases, spinal cord injury, stroke, and depression. Deep‐brain electrical stimulation, transcranial magnetic stimulation, and transcranial direct current stimulation, all promoted neurogenesis in experimental rodent models of these illnesses (Huang et al, ).…”

Section: Proliferation Migration Differentiation and Necrosis/apopmentioning

confidence: 99%

Regulation of adult neural progenitor cell functions by purinergic signaling

Tang

Illéš

2016

Glia

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Extracellular purines are signaling molecules in the neurogenic niches of the brain and spinal cord, where they activate cell surface purinoceptors at embryonic neural stem cells (NSCs) and adult neural progenitor cells (NPCs). Although mRNA and protein are expressed at NSCs/NPCs for almost all subtypes of the nucleotide-sensitive P2X/P2Y, and the nucleoside-sensitive adenosine receptors, only a few of those have acquired functional significance. ATP is sequentially degraded by ecto-nucleotidases to ADP, AMP, and adenosine with agonistic properties for distinct receptor-classes. Nucleotides/nucleosides facilitate or inhibit NSC/NPC proliferation, migration and differentiation. The most ubiquitous effect of all agonists (especially of ATP and ADP) appears to be the facilitation of cell proliferation, usually through P2Y1Rs and sometimes through P2X7Rs. However, usually P2X7R activation causes necrosis/apoptosis of NPCs. Differentiation can be initiated by P2Y2R-activation or P2X7R-blockade. A key element in the transduction mechanism of either receptor is the increase of the intracellular free Ca concentration, which may arise due to its release from intracellular storage sites (G protein-coupling; P2Y) or due to its passage through the receptor-channel itself from the extracellular space (ATP-gated ion channel; P2X). Further research is needed to clarify how purinergic signaling controls NSC/NPC fate and how the balance between the quiescent and activated states is established with fine and dynamic regulation. GLIA 2017;65:213-230.

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Electrical Stimulation Elicits Neural Stem Cells Activation: New Perspectives in CNS Repair

Cited by 54 publications

References 79 publications

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

Poly(3,4‐ethylenedioxythiophene):GlycosAminoGlycan Aqueous Dispersions: Toward Electrically Conductive Bioactive Materials for Neural Interfaces

Regulation of adult neural progenitor cell functions by purinergic signaling

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