Electrical slow waves in the mouse oviduct are dependent on extracellular and intracellular calcium sources

Dixon, Rose E.; Britton, Fiona C.; Baker, Salah A.; Hennig, Grant W.; Rollings, Christina; Sanders, Kenton M.; Ward, Sean M.

doi:10.1152/ajpcell.00293.2011

Cited by 15 publications

(16 citation statements)

References 60 publications

(79 reference statements)

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“…The fact that the majority of the dysregulated genes in 2PN and 2-cell embryos derived from ICSI using Dicer or Drosha cKO sperm are direct targets of miRNAs deficient or dysregulated in Dicer or Drosha cKO sperm strongly suggests that the paternal miRNAs act on their target mRNAs, which are mostly maternal transcripts, in the 2PN and 2-cell embryos. Many of the paternal miRNAs are also present in oocytes as the maternal miRNAs (Dixon et al, 2011;Hong et al, 2008). It remains puzzling that paternal miRNAs, rather than the same sets of maternal miRNAs, can affect maternal and early zygotic transcripts.…”

Section: Discussionmentioning

confidence: 99%

Sperm-borne miRNAs and endo-siRNAs are important for fertilization and preimplantation embryonic development

et al. 2015

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Although it is believed that mammalian sperm carry small noncoding RNAs (sncRNAs) into oocytes during fertilization, it remains unknown whether these sperm-borne sncRNAs truly have any function during fertilization and preimplantation embryonic development. Germlinespecific Dicer and Drosha conditional knockout (cKO) mice produce gametes (i.e. sperm and oocytes) partially deficient in miRNAs and/or endo-siRNAs, thus providing a unique opportunity for testing whether normal sperm ( paternal) or oocyte (maternal) miRNA and endosiRNA contents are required for fertilization and preimplantation development. Using the outcome of intracytoplasmic sperm injection (ICSI) as a readout, we found that sperm with altered miRNA and endo-siRNA profiles could fertilize wild-type (WT) eggs, but embryos derived from these partially sncRNA-deficient sperm displayed a significant reduction in developmental potential, which could be rescued by injecting WT sperm-derived total or small RNAs into ICSI embryos. Disrupted maternal transcript turnover and failure in early zygotic gene activation appeared to associate with the aberrant miRNA profiles in Dicer and Drosha cKO spermatozoa. Overall, our data support a crucial function of paternal miRNAs and/or endosiRNAs in the control of the transcriptomic homeostasis in fertilized eggs, zygotes and two-cell embryos. Given that supplementation of sperm RNAs enhances both the developmental potential of preimplantation embryos and the live birth rate, it might represent a novel means to improve the success rate of assisted reproductive technologies in fertility clinics.

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Section: Discussionmentioning

confidence: 99%

Sperm-borne miRNAs and endo-siRNAs are important for fertilization and preimplantation embryonic development

et al. 2015

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“…Previous studies of other visceral smooth muscle tissues have revealed that ICC pacemaker activity is dependent on [Ca 2þ ] o (extracellular Ca 2þ concentration) [9][10][11][12]. We have recently demonstrated that ICC-OVI pacemaker activity is also dependent on [Ca 2þ ] o because its reduction from 2.5 mM to nominally free [Ca 2þ ] o inhibited slow waves and myosalpinx contractions [13]. ICC-OVI pacemaker activity is also abolished by the sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) pump inhibitor cyclopiazonic acid, revealing that functional intracellular Ca 2þ stores are essential for pacemaker activity [13].…”

Section: Introductionmentioning

confidence: 87%

“…First-strand cDNA was prepared from 1 lg RNA using SuperScript II reverse transcriptase (Invitrogen) in a 20-ll reaction containing 25 ng oligo dT (12)(13)(14)(15)(16)(17)(18) primer, 1 ll of 10 mM dNTP, 53 first-strand buffer (250 mM Tris-HCl, pH 8.3, 375 mM KCl, 15 mM MgCl 2 ), and 0.1 M dithiothreitol. PCR was performed with specific primers for Tmem16a on 2 ll cDNA using AmpliTaq Gold PCR Master Mix (Applied Biosystems, Foster City, CA).…”

Section: Rt-pcrmentioning

confidence: 99%

“…We have recently demonstrated that ICC-OVI pacemaker activity is also dependent on [Ca 2þ ] o because its reduction from 2.5 mM to nominally free [Ca 2þ ] o inhibited slow waves and myosalpinx contractions [13]. ICC-OVI pacemaker activity is also abolished by the sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) pump inhibitor cyclopiazonic acid, revealing that functional intracellular Ca 2þ stores are essential for pacemaker activity [13]. Based on these findings, it seems likely that a depolarizing Ca 2þ -dependent conductance is involved in ICC-OVI-mediated pacemaker activity.…”

Section: Introductionmentioning

confidence: 99%

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Electrical Slow Waves in the Mouse Oviduct Are Dependent upon a Calcium Activated Chloride Conductance Encoded by Tmem16a1

Dixon¹,

Hennig²,

Baker³

et al. 2012

Biology of Reproduction

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Myosalpinx contractions are critical for oocyte transport along the oviduct. A specialized population of pacemaker cells-oviduct interstitial cells of Cajal-generate slow waves, the electrical events underlying myosalpinx contractions. The ionic basis of oviduct pacemaker activity is unknown. We examined the role of a new class of Ca(2+)-activated Cl(-) channels (CaCCs)-anoctamin 1, encoded by Tmem16a-in oviduct slow wave generation. RT-PCR revealed the transcriptional expression of Tmem16a-encoded CaCCs in the myosalpinx. Intracellular microelectrode recordings were performed in the presence of two pharmacologically distinct Cl(-) channel antagonists, anthracene-9-carboxylic acid and niflumic acid. Both of these inhibitors caused membrane hyperpolarization, reduced the duration of slow waves, and ultimately inhibited pacemaker activity. Niflumic acid also inhibited propagating calcium waves within the myosalpinx. Slow waves were present at birth in wild-type and heterozygous oviducts but failed to develop by birth in mice homozygous for a null allele of Tmem16a (Tmem16a(tm1Bdh/tm1Bdh)). These data suggest that Tmem16a-encoded CaCCs contribute to membrane potential and are responsible for the upstroke and plateau phases of oviduct slow waves.

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“…cDNA was amplified with a LightCycler Ò 480 SYBR Green I Master Kit and a LightCycler 480 system (both from Roche Applied Science). The primers used for the amplification of VGCC (Ca v 1.1-Ca v 1.3 and Ca v 3.1-Ca v 3.3) have been published before [20]. The PCRs were optimized to suit our conditions.…”

Section: Whole-cell Patch-clamp Recordingsmentioning

confidence: 99%

Role of Low Voltage Activated Calcium Channels in Neuritogenesis and Active Migration of Embryonic Neural Progenitor Cells

Louhivuori

Wigren

et al. 2013

Stem Cells and Development

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The central role of calcium influx and electrical activity in embryonic development raises important questions about the role and regulation of voltage-dependent calcium influx. Using cultured neural progenitor cell (NPC) preparations, we recorded barium currents through voltage-activated channels using the whole-cell configuration of the patch-clamp technique and monitored intracellular free calcium concentrations with Fura-2 digital imaging. We found that NPCs as well as expressing high-voltage-activated (HVA) calcium channels express functional low-threshold voltage-dependent calcium channels in the very early stages of differentiation (5 h to 1 day). The size of the currents recorded at -50 versus -20 mV after 1 day in differentiation was dependent on the nature of the charge carrier. Peak currents measured at -20 mV in the presence 10 mM Ca2+ instead of 10 mM Ba2+ had a tendency to be smaller, whereas the nature of the divalent species did not influence the amplitude measured at -50 mV. The T-type channel blockers mibefradil and NNC 55-0396 significantly reduced the calcium responses elicited by depolarizing with extracellular potassium, while the overall effect of the HVA calcium channel blockers was small at differentiation day 1. At differentiation day 20, the calcium responses were effectively blocked by nifedipine. Time-lapse imaging of differentiating neurospheres cultured in the presence of low-voltage-activated (LVA) blockers showed a significant decrease in the number of active migrating neuron-like cells and neurite extensions. Together, these data provide evidence that LVA calcium channels are involved in the physiology of differentiating and migrating NPCs.

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Electrical slow waves in the mouse oviduct are dependent on extracellular and intracellular calcium sources

Cited by 15 publications

References 60 publications

Sperm-borne miRNAs and endo-siRNAs are important for fertilization and preimplantation embryonic development

Sperm-borne miRNAs and endo-siRNAs are important for fertilization and preimplantation embryonic development

Electrical Slow Waves in the Mouse Oviduct Are Dependent upon a Calcium Activated Chloride Conductance Encoded by Tmem16a1

Role of Low Voltage Activated Calcium Channels in Neuritogenesis and Active Migration of Embryonic Neural Progenitor Cells

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