Electrical signaling in control of ocular cell behaviors

Zhao, Min; Chalmers, Laura; Cao, Lin; Vieira, Ana Carolina; Mannis, Mark J.; Reid, Brian

doi:10.1016/j.preteyeres.2011.10.001

Cited by 53 publications

(50 citation statements)

References 243 publications

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“…Similarly, electric fields, arising from the non-uniform distribution of ion tranlocating pumps and channels in the lens cells (Robinson and Patterson, 1982) could constitute a spatial cue. Work with cultured lens epithelial cells has shown cells orient themselves with respect to electric fields in the medium (Zhao et al, 2012). Moreover, the direction of field-induced cellular migration varied with the origin of the lens cells (i.e., whether they were harvested from the central or peripheral epithelium) (Wang et al, 2003).…”

Section: Signaling Network Implicated In the Regulation Of Lens Simentioning

confidence: 99%

The lens growth process

Bassnett

Šikić

2017

Progress in Retinal and Eye Research

102

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The factors that regulate the size of organs to ensure that they fit within an organism are not well understood. A simple organ, the ocular lens serves as a useful model with which to tackle this problem. In many systems, considerable variance in the organ growth process is tolerable. This is almost certainly not the case in the lens, which in addition to fitting comfortably within the eyeball, must also be of the correct size and shape to focus light sharply onto the retina. Furthermore, the lens does not perform its optical function in isolation. Its growth, which continues throughout life, must therefore be coordinated with that of other tissues in the optical train. Here, we review the lens growth process in detail, from pioneering clinical investigations in the late nineteenth century to insights gleaned more recently in the course of cell and molecular studies. During embryonic development, the lens forms from an invagination of surface ectoderm. Consequently, the progenitor cell population is located at the surface and differentiated cells are confined to the interior. The interactions that regulate cell fate thus occur within the obligate ellipsoidal geometry of the lens. In this context, mathematical models are particularly appropriate tools with which to examine the growth process. In addition to identifying key growth determinants, such models constitute a framework for integrating cell biological and optical data, helping clarify the relationship between gene expression in the lens and image quality at the retinal plane.

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Section: Signaling Network Implicated In the Regulation Of Lens Simentioning

confidence: 99%

The lens growth process

Bassnett

Šikić

2017

Progress in Retinal and Eye Research

102

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“…Epithelia engaged in active electrolyte transport typically generate spontaneous transepithelial potentials (TEP) that provide an electrical driving force for paracellular ion movement across the epithelium [89]. Following wounding, the TEP at the site of the wound collapses as laterally oriented electric fields develop with the cathode (negative pole) located at the center of the wound.…”

Section: Electrotaxismentioning

confidence: 99%

CFTR Involvement in Cell Migration and Epithelial Restitution

O’Grady¹

2017

Progress in Understanding Cystic Fibrosis

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Over the past decade, research has shown that cystic fibrosis transmembrane conductance regulator (CFTR) plays an important role in epithelial cell migration and wound healing. Experiments with airway epithelium, ovarian epithelial cells, placental epithelium and epidermal keratinocytes demonstrated that CFTR function is necessary to achieve maximum migration rates during restitution and in certain cancer cells, CFTR activity contributes to tumor cell invasion. Multiple mechanisms appear to underlie the motility-promoting actions of CFTR, and although many details remain to be established, our present understanding indicates that processes such as electrotaxis (galvanotaxis), integrin-mediated cell adhesion and lamellipodia protrusion are dependent on normal CFTR function. In this chapter, the role of CFTR in epithelial cell migration and its implications in cystic fibrosis (CF) will be reviewed with emphasis on the underlying mechanisms that may explain observations made in various epithelial tissues, particularly in airways. Ultimately, a better understanding of CFTR involvement in epithelial repair may lead to new therapeutic approaches to improve barrier function and reduce airway infection and inflammation associated with CF.

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“…Therefore, coupling of an electromagnetic field with the live cell can occur via either field interaction with charged molecules and proteins in the cell membrane that alters the flow of ions through the ion channels or rearranges the distribution of the membrane receptors, or via direct field penetration inside the cell and interaction with charged entities in the cytoplasm [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Modulation of cell function by electric field: a high-resolution analysis

Taghian

Narmoneva

Kogan

2015

J. R. Soc. Interface.

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Regulation of cell function by a non-thermal, physiological-level electromagnetic field has potential for vascular tissue healing therapies and advancing hybrid bioelectronic technology. We have recently demonstrated that a physiological electric field (EF) applied wirelessly can regulate intracellular signalling and cell function in a frequency-dependent manner. However, the mechanism for such regulation is not well understood. Here, we present a systematic numerical study of a cell-field interaction following cell exposure to the external EF. We use a realistic experimental environment that also recapitulates the absence of a direct electric contact between the field-sourcing electrodes and the cells or the culture medium. We identify characteristic regimes and present their classification with respect to frequency, location, and the electrical properties of the model components. The results show a striking difference in the frequency dependence of EF penetration and cell response between cells suspended in an electrolyte and cells attached to a substrate. The EF structure in the cell is strongly inhomogeneous and is sensitive to the physical properties of the cell and its environment. These findings provide insight into the mechanisms for frequency-dependent cell responses to EF that regulate cell function, which may have important implications for EF-based therapies and biotechnology development.

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Electrical signaling in control of ocular cell behaviors

Cited by 53 publications

References 243 publications

The lens growth process

The lens growth process

CFTR Involvement in Cell Migration and Epithelial Restitution

Modulation of cell function by electric field: a high-resolution analysis

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