Electrical pulse stimulation of skeletal myoblasts cell cultures with simulated action potentials

Villanueva, Paula; Pereira, Sheila M.; Olmo, Alberto; Pérez, Pablo; Yuste, Yaiza; Yufera, A.; Portilla, Fernando de la

doi:10.1002/term.2869

Cited by 13 publications

(11 citation statements)

References 11 publications

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“…An interesting application involves skeletal muscle tissue engineering, which seeks to drive myogenesis, the differentiation of myoblasts into myotubes. Ultrasoft gelatin hydrogel substrates were earlier crosslinked with mTG for myoblast alignment. , However, the use of enzymatically crosslinked gelatin to incorporate electroactivity, a vital factor to enable cell signaling, development of mature sarcomeric structure, and functionality such as contractibility, is relatively unexplored. , Here, we explored biocompatible, conducting mTG–gelatin as a way to incorporate electroactivity without adversely affecting cell growth and proliferation.…”

Section: Resultsmentioning

confidence: 99%

Stretchable and Electroactive Crosslinked Gelatin for Biodevice and Cell Culture Applications

Brooks

Wulff

Broitman

et al. 2022

ACS Appl. Bio Mater.

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Biomimetic substrates that incorporate functionality such as electroactivity and mechanical flexibility, find utility in a variety of biomedical applications. Toward these uses, naturederived materials such as gelatin offer inherent biocompatibility and sustainable sourcing. However, issues such as high swelling, poor mechanical properties, and lack of stability at biological temperatures limit their use. The enzymatic crosslinking of gelatin via microbial transglutaminase (mTG) yields flexible and robust large area substrates that are stable under physiological conditions. Here, we demonstrate the fabrication and characterization of strong, stretchable, conductive mTG crosslinked gelatin thin films. Incorporation of the conductive polymer poly(3,4-ethylenedioxythiophene) polystyrene sulfonate in the gel matrix with a bioinspired polydopamine surface coating is used to enable conductivity with enhanced mechanical properties such as extensibility and flexibility, in comparison to plain gelatin or crosslinked gelatin films. The electroconductive substrates are conducive to cell growth, supporting myoblast cell adhesion, viability, and proliferation and could find use in creating active cell culture systems incorporating electrical stimulation. The substrates are responsive to motion such as stretching and bending while being extremely handleable and elastic, making them useful for applications such as electronic skin and flexible bioelectronics. Overall, this work presents facile, yet effective development of bioinspired conductive composites as substrates for bio-integrated devices and functional tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Stretchable and Electroactive Crosslinked Gelatin for Biodevice and Cell Culture Applications

Brooks

Wulff

Broitman

et al. 2022

ACS Appl. Bio Mater.

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show abstract

“…Therefore, cardiac cells' alignment, electrical coupling, and phenotype maintenance are highly improved under pulsatile currents 29,30 . Electrical pulse stimulation has also been reported to enhance the proliferation and differentiation of skeletal myoblasts 7,31 . Relatively few studies apply DC to treat myoblasts.…”

Section: Discussionmentioning

confidence: 99%

Development of a multifunctional bioreactor to evaluate the promotion effects of cyclic stretching and electrical stimulation on muscle differentiation

Hu,

Chen,

Tsao

et al. 2023

Bioengineering & Transla Med

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A multifunctional bioreactor was fabricated in this study to investigate the facilitation efficiency of electrical and mechanical stimulations on myogenic differentiation. This bioreactor consisted of a highly stretchable conductive membrane prepared by depositing polypyrrole (PPy) on a flexible polydimethylsiloxane (PDMS) film. The tensile deformation of the PPy/PDMS membrane can be tuned by adjusting the channel depth. In addition, PPy/PDMS maintained its electrical conductivity under continuous cyclic stretching in the strain range of 6.5%–13% for 24 h. This device can be used to individually or simultaneously perform cyclic stretching and electrical stimulation. The results of single stimulation showed that either cyclic stretching or electrical stimulation upregulated myogenic gene expression and promoted myotube formation, where electrical stimulation improved better than cyclic stretching. However, only cyclic stretching can align C2C12 cells perpendicular to the stretching direction, and electrical stimulation did not affect cell morphology. Myosin heavy chain (MHC) immunostaining demonstrated that oriented cells under cyclic stretching resulted in parallel myotubes. The combination of these two stimuli exhibited synergetic effects on both myogenic gene regulation and myotube formation, and the incorporated electrical field did not affect the orientation effect of the cyclic stretching. These results suggested that these two treatments likely influenced cells through different pathways. Overall, the simultaneous application of cyclic stretching and electrical stimulation preserved both stimuli's advantages, so myo‐differentiation can be highly improved to obtain abundant parallel myotubes, suggesting that our developed multifunctional bioreactor should benefit muscle tissue engineering applications.

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“…In tissue engineering, the control of tissue formation is very important to form tissue constructs that can be used in clinical practice [ 33 ]. Optimization of tissue engineering to develop functional muscle requires a complex strategy combining metabolic optimization, stimulation with soluble factors, and biophysical stimulation [ 34 ].…”

Section: 3d-printed Actuators In Tissue Engineeringmentioning

confidence: 99%

“…Electrical pulse stimulation (EPS) has been applied to induce cell clustering in cultured neural networks [ 35 ] or has improved skeletal muscle regeneration through satellite cell fusion with myofibers in healthy elderly subjects [ 36 ], among other applications. In [ 33 ], electrical pulse stimulation (EPS) was used to improve skeletal muscle development and maturation. In this work, it was shown how important the geometry of electrodes or the type of signal used in electrostimulation can be, producing different cellular development and tissue formation.…”

Section: 3d-printed Actuators In Tissue Engineeringmentioning

confidence: 99%

3D-Printed Sensors and Actuators in Cell Culture and Tissue Engineering: Framework and Research Challenges

Pérez

Serrano

Olmo

2020

Sensors

Self Cite

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Three-dimensional printing technologies have been recently proposed to monitor cell cultures and implement cell bioreactors for different biological applications. In tissue engineering, the control of tissue formation is crucial to form tissue constructs of clinical relevance, and 3D printing technologies can also play an important role for this purpose. In this work, we study 3D-printed sensors that have been recently used in cell culture and tissue engineering applications in biological laboratories, with a special focus on the technique of electrical impedance spectroscopy. Furthermore, we study new 3D-printed actuators used for the stimulation of stem cells cultures, which is of high importance in the process of tissue formation and regenerative medicine. Key challenges and open issues, such as the use of 3D printing techniques in implantable devices for regenerative medicine, are also discussed.

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Electrical pulse stimulation of skeletal myoblasts cell cultures with simulated action potentials

Cited by 13 publications

References 11 publications

Stretchable and Electroactive Crosslinked Gelatin for Biodevice and Cell Culture Applications

Stretchable and Electroactive Crosslinked Gelatin for Biodevice and Cell Culture Applications

Development of a multifunctional bioreactor to evaluate the promotion effects of cyclic stretching and electrical stimulation on muscle differentiation

3D-Printed Sensors and Actuators in Cell Culture and Tissue Engineering: Framework and Research Challenges

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