Electrical Pulse-Mediated Veliparib for Effective Treatment of Triple Negative Breast Cancer: An in vitro Model Study

Abstract

Article InfoUse of Poly (ADP-ribose) polymerase (PARP) inhibitors has received major attention as therapeutic agents for the treatment of breast cancers with or without mutations in BRCA1 or BRCA2. Thus, it can be effectively used for triple negative breast cancer (TNBC), which lacks the conventional receptors that many chemo drugs attack. Veliparib, a PARP agent is used as 2 nd line of treatment along with platinum compounds. It is of practical interest to study the efficacy of Veliparib alone as an anticancer drug for TNBC. Towards this, in this research, we studied the efficacy of Veliparib on MDA-MB-231, human triple negative breast cancer cells. A concentration of 330µM was used for this purpose. In addition, to enhance the uptake of Veliparib against the plasma membranes of the cells, electroporation technique is used, which involves the local application of electrical pulses to open pores, which enables easy drug passage across the cell membranes. Thus, the objective of this study is to identify the potential of Veliparib and Electroporation as an alternate combinational therapy for TNBCs. Electrical pulses of high intensity, low duration 1200V/cm, 100μs, 8 pulses and low intensity high duration 500V/cm, 20ms, 8 pulses are used in this study. Cell viabilities were measured immediately, as well as after 24, 48 and 72 hours of treatment. The results indicate cell viabilities of 70% immediately after Veliparib+electrical pulses treatment, compared to 94% with drug only, indicating the potential of the synergy of electrical pulses+Veliparib. The viabilities were lower by 11 to 13 times after 72 hours. This promising treatment is transferrable to clinical practice.

Viability and cell cycle studies of metastatic triple negative breast cancer cells using low voltage electrical pulses and herbal curcumin

“…We used curcumin in the present study, as we had started investigating a number of commonly administered drugs, including tamoxifen, paclitaxel, doxorubicin, bleomycin, cisplatin, gemcitabine, and veliparib [17][18][19][20] using electric pulses (EPs) and various breast cancer cells since 2007. We have recently started studying curcumin due to its excellent anticancerous, anti-oxidant, and anti-bacterial qualities, as well as its effectiveness for treating several chronic conditions, including cancer [21].…”

The Chemopreventive Effects of The Combination Between Tea Leaf and Mandarine Peel Extract on Breast Cancer Cell

“…This method was based on the breakdown of tetrazolium salt or yellow MTT (3-(4,5-dimethylthyazol-2-yl) -2,5diphenyl tetrazolium bromide) by dehydrogenase succinate enzyme contained in the cell mitochondria to turn into purplish blue formazan crystals. 13 Cells with a density of 70-80% (confluent) were distributed into 96 well plates, observed under an inverted microscope to identify the cells' distribution, and then incubated for 48 hours so that the cells could adapt and attach to the bottom of the wells. On the following day, the media in the wells were dumped by turning the well plates over the dump, then drained with a tissue.…”