Electrical properties of cells from human olfactory epithelium

Tamari, Kengo; Takeuchi, Hiroko; Kobayashi, Masayoshi; Takeuchi, Kazuhiko; Kurahashi, Takashi; Yamamoto, Tetsuro

doi:10.1016/j.anl.2019.01.006

Cited by 3 publications

(4 citation statements)

References 26 publications

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“…The resting electrical properties, input resistance and resting membrane potential, that we measured from human olfactory sensory neurons in slices were found to be similar to those reported by Restrepo et al 50 from isolated human olfactory neurons and to those measured in other vertebrates 59 . However, in contrast to previous reports that recorded inward voltage-gated currents in only one out of eleven 50 or one out of fourteen 58 freshly dissociated human olfactory sensory neurons, we consistently recorded both voltage-gated transient inward currents and outward currents in olfactory neurons that were clearly visualized with a fluorescence dye. Our results suggest that avoiding enzymatic dissociation may be crucial for preserving the electrophysiological properties of these neurons.…”

Section: Discussioncontrasting

confidence: 99%

“…Both studies consistently measured outward voltage-gated currents in response to depolarizing voltage steps, while transient inward currents were rarely observed in human olfactory neurons. The absence of transient inward currents in most human olfactory neurons is surprising, as they are found in other vertebrates 58 , and this may be due either to a unique aspect of human olfactory transduction 57 or to neuron damage during the dissociation procedure 49 .…”

Section: Introductionmentioning

confidence: 97%

“…One of these studies used the inside-out patch-clamp technique from the dendritic knob of dissociated human olfactory neurons to characterize activation of CNG channels by cAMP, providing evidence that these channels may be involved in olfactory transduction in humans 57 . Two other reports investigated the electrical properties of isolated human olfactory neurons with the whole-cell patch-clamp technique 50,58 . Both studies consistently measured outward voltage-gated currents in response to depolarizing voltage steps, while transient inward currents were rarely observed in human olfactory neurons.…”

Section: Introductionmentioning

confidence: 99%

“…In rodents and amphibians, electrophysiological techniques have been extensively used to study the functional properties of olfactory sensory neurons while only a few studies have been reported in humans 50,57,58 . One of these studies used the inside-out patch-clamp technique from the dendritic knob of dissociated human olfactory neurons to characterize activation of CNG channels by cAMP, providing evidence that these channels may be involved in olfactory transduction in humans 57 .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Shedding light on human olfaction: electrophysiological recordings from sensory neurons in acute slices of olfactory epithelium

Hernandez-Clavijo

Sánchez-Triviño

Guarneri

et al. 2023

Preprint

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The COVID-19 pandemic brought attention to our limited understanding of human olfactory physiology. While the cellular composition of the human olfactory epithelium is similar to that of other vertebrates, its functional properties are largely unknown. We prepared acute slices of human olfactory epithelium from nasal biopsies and used the whole-cell patch-clamp technique to record electrical properties of cells. We measured voltage-gated currents in human olfactory sensory neurons and supporting cells, and action potentials in neurons. Additionally, inward currents and action potentials responses of neurons to a phosphodiesterase inhibitor indicated that the transduction cascade involves cAMP as a second messenger. Furthermore, responses to odorant mixtures demonstrated that the transduction cascade was intact in this preparation. This study provides the first electrophysiological characterization of olfactory sensory neurons in acute slices of the human olfactory epithelium, paving the way for future research to expand our knowledge of human olfactory physiology.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Shedding light on human olfaction: electrophysiological recordings from sensory neurons in acute slices of olfactory epithelium

Hernandez-Clavijo

Sánchez-Triviño

Guarneri

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Shedding light on human olfaction: Electrophysiological recordings from sensory neurons in acute slices of olfactory epithelium

Hernandez-Clavijo¹,

Triviño²,

Guarneri³

et al. 2023

iScience

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Somatic ion channels and action potentials in olfactory receptor cells and vomeronasal receptor cells

Kawai

2024

Journal of Neurophysiology

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Olfactory receptor cells are primary sensory neurons that catch odor molecules in olfactory system, and vomeronasal receptor cells catch pheromones in vomeronasal system. When odor or pheromone molecules bind to receptor proteins expressed on the membrane of the olfactory cilia or vomeronasal microvilli, receptor potentials are generated in their receptor cells. This initial excitation is transmitted to the soma via dendrites, and action potentials are generated in the soma and/or axon and transmitted to the central nervous system. Thus, olfactory and vomeronasal receptor cells play an important role in converting chemical signals into electrical signals. In this review, the electrophysiological characteristics of ion channels in the somatic membrane of olfactory receptor cells and vomeronasal receptor cells in various species are described, and the differences between the action potential dynamics of olfactory receptor cells and vomeronasal receptor cells are compared

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Electrical properties of cells from human olfactory epithelium

Cited by 3 publications

References 26 publications

Shedding light on human olfaction: electrophysiological recordings from sensory neurons in acute slices of olfactory epithelium

Shedding light on human olfaction: electrophysiological recordings from sensory neurons in acute slices of olfactory epithelium

Shedding light on human olfaction: Electrophysiological recordings from sensory neurons in acute slices of olfactory epithelium

Somatic ion channels and action potentials in olfactory receptor cells and vomeronasal receptor cells

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