Electrical muscle stimulation elevates intramuscular BDNF and GDNF mRNA following peripheral nerve injury and repair in rats

Willand, Michael P.; Rosa, Elyse; Michalski, Bernadeta; Zhang, Jennifer J.; Gordon, Tessa; Fahnestock, Margaret; Borschel, Gregory H.

doi:10.1016/j.neuroscience.2016.07.040

Cited by 61 publications

(48 citation statements)

References 75 publications

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“…Electrical stimulation elevates intramuscular trophic factor mRNA levels which may explain how electrical stimulation enhances neural regeneration following nerve injury. 57 In addition, the impact of stimulation on terminal sprouting, a mentioned negative aspect of electrical muscle stimulation, was a minor contributor to long term functional reinnervation of stimulated muscles in that study. 56 …”

Section: Co-expression Of Fast and Slow Mchs Is Detected In Muscle Bimentioning

confidence: 62%

Use it or lose it: tonic activity of slow motoneurons promotes their survival and preferentially increases slow fiber-type groupings in muscles of old lifelong recreational sportsmen

et al. 2016

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Histochemistry, immuno-histochemistry, gel electrophoresis of single muscle fibers and electromyography of aging muscles and nerves suggest that: i) denervation contributes to muscle atrophy, ii) impaired mobility accelerates the process, and iii) lifelong running protects against loss of motor units. Recent corroborating results on the muscle effects of Functional Electrical Stimulation (FES) of aged muscles will be also mentioned, but we will in particular discuss how and why a lifelong increased physical activity sustains reinnervation of muscle fibers. By analyzing distribution and density of muscle fibers co-expressing fast and slow Myosin Heavy Chains (MHC) we are able to distinguish the transforming muscle fibers due to activity related plasticity, to those that adapt muscle fiber properties to denervation and reinnervation. In muscle biopsies from septuagenarians with a history of lifelong high-level recreational activity we recently observed in comparison to sedentary seniors: 1. decreased proportion of small-size angular myofibers (denervated muscle fibers); 2. considerable increase of fiber-type groupings of the slow type (reinnervated muscle fibers); 3. sparse presence of muscle fibers co-expressing fast and slow MHC. Immuno-histochemical characteristics fluctuate from those with scarce fiber-type modulation and groupings to almost complete transformed muscles, going through a process in which isolated fibers co-expressing fast and slow MHC fill the gaps among fiber groupings. Data suggest that lifelong high-level exercise allows the body to adapt to the consequences of the age-related denervation and that it preserves muscle structure and function by saving otherwise lost muscle fibers through recruitment to different slow motor units. This is an opposite behavior of that described in long term denervated or resting muscles. These effects of lifelong high level activity seems to act primarily on motor neurons, in particular on those always more active, i.e., on the slow motoneurons. The preferential reinnervation that follows along decades of increased activity maintains neuron and myofibers. All together the results open interesting perspectives for applications of FES and electroceuticals for rejuvenation of aged muscles to delay functional decline and loss of independence that are unavoidable burdens of advanced aging.Trial Registration: ClinicalTrials.gov: NCT01679977

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Section: Co-expression Of Fast and Slow Mchs Is Detected In Muscle Bimentioning

confidence: 62%

Use it or lose it: tonic activity of slow motoneurons promotes their survival and preferentially increases slow fiber-type groupings in muscles of old lifelong recreational sportsmen

et al. 2016

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“…The release of neurotrophic factors is thus considered as an important index for evaluating the normal biological function of Schwann cells. Therefore, two key growth factors, BDNF and GDNF, were mainly measured in the present study, as these growth factors could be released by Schwann cells and were found to have positive effect on peripheral nerve regeneration in the previous study . The released amount of BDNF and GDNF by Schwann cells in various PCL scaffolds after culture for 24 h is shown in Figure (a).…”

Section: Resultsmentioning

confidence: 84%

Fabrication of alignment polycaprolactone scaffolds by combining use of electrospinning and micromolding for regulating Schwann cells behavior

Zhang

Chen

Liang

et al. 2018

J Biomedical Materials Res

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In the present study, a new approach for fabricating micropatterned polycaprolactone (PCL) scaffolds with ridge/groove structure on the surface was developed by combining use of electrospinning and micromolding method. A series of physicochemical properties, including morphology, wettability, component, crystal pattern and mechanical properties, of prepared PCL scaffolds were characterization, respectively. Stability of the micropatterned PCL scaffolds was measured using phosphate buffer solution immersion for a certain period. Then, the regulating effects of the micropatterned PCL scaffolds on attachment, orientation and normal biological function of Schwann cells were evaluated. And the protein adsorption behavior in various PCL scaffolds was also detected. The results showed that the micropatterned PCL scaffolds demonstrated a porous micro/nano complex structure with enhanced hydrophobicity and mechanical properties as a function of electrospun flow-rate of PCL solution. The micropatterned PCL scaffolds possessed good stability and could effectively regulate the attachment and orientation of Schwann cells at the early stage after cell culture. Importantly, the electrospun flow-rate of PCL solution was found to play an important role in scaffold properties, cell behavior and protein adsorption. The micropatterned scaffolds with a flow-rate of PCL solution at 0.12 mL h demonstrated the better regulation on Schwann cells attachment and alignment without negatively affect the normal biological function of the cells. To the best of our knowledge, this is the first report of combining use of electrospinning and micromolding method for preparing artificial nerve implants. The study is anticipated to have potential application in peripheral nerve and other tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3123-3134, 2018.

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“…The expression of stress and senescence markers was evaluated by staining sections for senescence‐associated β‐galactosidase (SA‐β‐gal) activity and p16 INK4A expression . The gene expression of select neurotrophic factors ( Gdnf and Bdnf ) was analyzed using qRT‐PCR, where Gdnf and Bdnf expression are known to have a major role in promoting nerve regeneration …”

Section: Methodsmentioning

confidence: 99%

Nerve stepping stone has minimal impact in aiding regeneration across long acellular nerve allografts

et al. 2017

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Electrical muscle stimulation elevates intramuscular BDNF and GDNF mRNA following peripheral nerve injury and repair in rats

Cited by 61 publications

References 75 publications

Use it or lose it: tonic activity of slow motoneurons promotes their survival and preferentially increases slow fiber-type groupings in muscles of old lifelong recreational sportsmen

Use it or lose it: tonic activity of slow motoneurons promotes their survival and preferentially increases slow fiber-type groupings in muscles of old lifelong recreational sportsmen

Fabrication of alignment polycaprolactone scaffolds by combining use of electrospinning and micromolding for regulating Schwann cells behavior

Nerve stepping stone has minimal impact in aiding regeneration across long acellular nerve allografts

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