Electrical Lysis and RNA Extraction from Single Cells Fixed by Dithiobis(succinimidyl propionate)

Parimalam, Sangamithirai Subramanian; Oguchi, Yusuke; Abdelmoez, Mahmoud N.; Tsuchida, Arata; Ozaki, Yuka; Yokokawa, Ryuji; Kotera, Hidetoshi; Shintaku, Hirofumi

doi:10.1021/acs.analchem.8b02338

Cited by 16 publications

(18 citation statements)

References 31 publications

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“…Hence, nucleic acids can be extracted and pre-concentrated in the presence of a leading electrolyte (LE) and a tailing electrolyte (TE) ( Fig. 7 A) ( Subramanian Parimalam et al, 2018 ). Another nucleic acid extraction method is ion concentration polarization (ICP)-based electrokinetic (EK) trapping, in which the selective transport of cations through a cation-selective membrane (CEM), under a DC electric field, induces an ion depletion zone with significantly amplified electric fields in the microchannel; this acts as an electric force barrier that prevents the passage of negatively charged species ( Ouyang et al, 2018 ).…”

Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

Detection of COVID-19: A review of the current literature and future perspectives

Liu

Wang

et al. 2020

Biosensors and Bioelectronics

337

309

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The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to the coronavirus disease 2019 (COVID-19) worldwide pandemic. This unprecedented situation has garnered worldwide attention. An effective strategy for controlling the COVID-19 pandemic is to develop highly accurate methods for the rapid identification and isolation of SARS-CoV-2 infected patients. Many companies and institutes are therefore striving to develop effective methods for the rapid detection of SARS-CoV-2 ribonucleic acid (RNA), antibodies, antigens, and the virus. In this review, we summarize the structure of the SARS-CoV-2 virus, its genome and gene expression characteristics, and the current progression of SARS-CoV-2 RNA, antibodies, antigens, and virus detection. Further, we discuss the reasons for the observed false-negative and false-positive RNA and antibody detection results in practical clinical applications. Finally, we provide a review of the biosensors which hold promising potential for point-of-care detection of COVID-19 patients. This review thereby provides general guidelines for both scientists in the biosensing research community and for those in the biosensor industry to develop a highly sensitive and accurate point-of-care COVID-19 detection system, which would be of enormous benefit for controlling the current COVID-19 pandemic.

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Section: Potential Biosensors For Point-of-care Sars-cov-2 Detectionmentioning

confidence: 99%

Detection of COVID-19: A review of the current literature and future perspectives

Liu

Wang

et al. 2020

Biosensors and Bioelectronics

337

309

View full text Add to dashboard Cite

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“…Reagents to support stable entrapment or covalent crosslinking of analytes within cells with on-demand release also prolong potential sample storage times prior to assay and increase retrieval or extraction efficiency. In recent years, this nascent field has experienced an explosion of novel protocols for performing a wide array of single-cell bioanalytical assays including immunohistochemistry, proteomic measurements, and DNA/RNA-sequencing [68,[85][86][87][88][89]. The following sections will serve to characterize the most significant recent developments of innovative single-cell preservation protocols.…”

Section: Novel Chemical Chemoselective and Reversible Single-cell Preservation Strategiesmentioning

confidence: 99%

“…4A) [91]. Reagents such as homobifunctional NHS-ester crosslinking reagents are more selective and less reactive than aldehydes; these reagents have been used to successfully preserve single cells while maintaining cellular structure and surrounding membranes, facilitating RNA-seq on single cells stored at 4°C up to 3 days post-fixation [89,96].…”

Section: Chemoselective Single-cell Preservation Strategiesmentioning

confidence: 99%

Preserving single cells in space and time for analytical assays

Gallion

Anttila

Abraham

et al. 2020

TrAC Trends in Analytical Chemistry

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Analytical assays performed within clinical laboratories influence roughly 70% of all medical decisions by facilitating disease detection, diagnosis, and management. Both in clinical and academic research laboratories, single-cell assays permit measurement of cell diversity and identification of rare cells, both of which are important in the understanding of disease pathogenesis. For clinically utility, the single-cell assays must be compatible with the clinical workflow steps of sample collection, sample transportation, pre-analysis processing, and singlecell assay; therefore, it is paramount to preserve cells in a state that resembles that in vivo rather than measuring signaling behaviors initiated in response to stressors such as sample collection and processing. To address these challenges, novel cell fixation (and more broadly, cell preservation) techniques incorporate programmable fixation times, reversible bond formation and cleavage, chemoselective reactions, and improved analyte recovery. These technologies will further the development of individualized, precision therapies for patients to yield improved clinical outcomes.

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“…Shintaku's group reported a microfluidic device containing a hydrodynamic trap for electrical lysis (Subramanian Parimalam et al, 2018). All on-chip processes were completed in <5 min.…”

Section: Collection Of Subcellular Cytoplasmmentioning

confidence: 99%

Electric and Electrochemical Microfluidic Devices for Cell Analysis

et al. 2019

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Microfluidic devices are widely used for cell analysis, including applications for single-cell analysis, healthcare, environmental monitoring, and organs-on-a-chip that mimic organs in microfluidics. Moreover, to enable high-throughput cell analysis, real-time monitoring, and non-invasive cell assays, electric and electrochemical systems have been incorporated into microfluidic devices. In this mini-review, we summarize recent advances in these systems, with applications from single cells to three-dimensional cultured cells and organs-on-a-chip. First, we summarize microfluidic devices combined with dielectrophoresis, electrophoresis, and electrowetting-on-a-dielectric for cell manipulation. Next, we review electric and electrochemical assays of cells to determine chemical section activity, and oxygen and glucose consumption activity, among other applications. In addition, we discuss recent devices designed for the electric and electrochemical collection of cell components from cells. Finally, we highlight the future directions of research in this field and their application prospects.

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Electrical Lysis and RNA Extraction from Single Cells Fixed by Dithiobis(succinimidyl propionate)

Cited by 16 publications

References 31 publications

Detection of COVID-19: A review of the current literature and future perspectives

Detection of COVID-19: A review of the current literature and future perspectives

Preserving single cells in space and time for analytical assays

Electric and Electrochemical Microfluidic Devices for Cell Analysis

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