Electrical impedance sensor for quantitative monitoring of infection processes on HCT-8 cells by the waterborne parasite Cryptosporidium

Dibao-Dina, Alfred; Follet, Jérôme; Ibrahim, Magdy A. M.; Vlandas, Alexis; Senez, V.

doi:10.1016/j.bios.2014.11.009

Cited by 22 publications

(12 citation statements)

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“…It has been shown that an electrical impedance-based device is able to provide insights on Cryptosporidium development in HCT-8 cell cultures. By quantifying the magnitude of the impedimetric response, this device can be used as an infectivity sensor as early as 12 h post-infection [ 52 ]. Cell culture assays have been reported to achieve a sensitivity of one single infectious oocyst and are unlikely to be subject to false-positive results [ 117 ].…”

Section: Evaluation Of Infectivitymentioning

confidence: 99%

Assessing viability and infectivity of foodborne and waterborne stages (cysts/oocysts) of Giardia duodenalis, Cryptosporidium spp., and Toxoplasma gondii: a review of methods

et al. 2018

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Giardia duodenalis, Cryptosporidium spp. and Toxoplasma gondii are protozoan parasites that have been highlighted as emerging foodborne pathogens by the Food and Agriculture Organization of the United Nations and the World Health Organization. According to the European Food Safety Authority, 4786 foodborne and waterborne outbreaks were reported in Europe in 2016, of which 0.4% were attributed to parasites including Cryptosporidium, Giardia and Trichinella. Until 2016, no standardized methods were available to detect Giardia, Cryptosporidium and Toxoplasma (oo)cysts in food. Therefore, no regulation exists regarding these biohazards. Nevertheless, considering their low infective dose, ingestion of foodstuffs contaminated by low quantities of these three parasites can lead to human infection. To evaluate the risk of protozoan parasites in food, efforts must be made towards exposure assessment to estimate the contamination along the food chain, from raw products to consumers. This requires determining: (i) the occurrence of infective protozoan (oo)cysts in foods, and (ii) the efficacy of control measures to eliminate this contamination. In order to conduct such assessments, methods for identification of viable (i.e. live) and infective parasites are required. This review describes the methods currently available to evaluate infectivity and viability of G. duodenalis cysts, Cryptosporidium spp. and T. gondii oocysts, and their potential for application in exposure assessment to determine the presence of the infective protozoa and/or to characterize the efficacy of control measures. Advantages and limits of each method are highlighted and an analytical strategy is proposed to assess exposure to these protozoa.

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Section: Evaluation Of Infectivitymentioning

confidence: 99%

Assessing viability and infectivity of foodborne and waterborne stages (cysts/oocysts) of Giardia duodenalis, Cryptosporidium spp., and Toxoplasma gondii: a review of methods

et al. 2018

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“…parvum oocysts Iowa (purchased from Waterborne™, New Orleans, LA) were stored in phosphate-buffered saline (PBS) with penicillin, streptomycin, gentamycin, amphotericin B and 0.001% Tween 20 at 4 °C until use. Oocyst viability and the estimation of the excitation rate were assessed by a protocol as previously described 50 . PBS complemented with 0.25% (wt/vol) trypsin and 0.75% (wt/vol) taurocholic acid were used to incubate the oocysts for 1 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: Proof of concept

Baydoun¹,

Benamrouz-Vanneste²,

Creusy³

et al. 2017

Sci Rep

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Cryptosporidium parvum is a major cause of diarrheal illness and was recently potentially associated with digestive carcinogenesis. Despite its impact on human health, Cryptosporidium pathogenesis remains poorly known, mainly due to the lack of a long-term culture method for this parasite. Thus, the aim of the present study was to develop a three-dimensional (3D) culture model from adult murine colon allowing biological investigations of the host-parasite interactions in an in vivo-like environment and, in particular, the development of parasite-induced neoplasia. Colonic explants were cultured and preserved ex vivo for 35 days and co-culturing was performed with C. parvum. Strikingly, the resulting system allowed the reproduction of neoplastic lesions in vitro at 27 days post-infection (PI), providing new evidence of the role of the parasite in the induction of carcinogenesis. This promising model could facilitate the study of host-pathogen interactions and the investigation of the process involved in Cryptosporidium-induced cell transformation.

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“…Currently, information about compact imaging systems incorporating a chip-scale microscope brings light to the diagnosis of major enteric parasites to save many lives [168]. Also, devices can track infectivity as early as 12 h post-infection, faster than other state of the art techniques [169]. Therefore, efforts are needed to facilitate market entry of these new technologies and facilitate mechanisms for their implementation.…”

Section: The Critical Role Of Biosensors In Important Parasitic Diseamentioning

confidence: 99%

Sensing parasites: Proteomic and advanced bio-detection alternatives

Sánchez-Ovejero

Benito‐Lopez

Díez

et al. 2016

Journal of Proteomics

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Journal of Proteomics 136 : 145-156 (2016) This work is made available online in accordance with publisher policies. To see the final version of this work please visit the publisher's website. Access to the published online version may require a subscription. This review provides the most recent methodological and technological advances with great potential for bio-sensing parasites in their hosts, showing the newest opportunities offered by modern "-omics" and platforms for parasite detection and control. Link to publisher's version

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Electrical impedance sensor for quantitative monitoring of infection processes on HCT-8 cells by the waterborne parasite Cryptosporidium

Cited by 22 publications

References 50 publications

Assessing viability and infectivity of foodborne and waterborne stages (cysts/oocysts) of Giardia duodenalis, Cryptosporidium spp., and Toxoplasma gondii: a review of methods

Assessing viability and infectivity of foodborne and waterborne stages (cysts/oocysts) of Giardia duodenalis, Cryptosporidium spp., and Toxoplasma gondii: a review of methods

Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: Proof of concept

Sensing parasites: Proteomic and advanced bio-detection alternatives

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