Zinc ion is closely related to human health. Its content in human body is small, while the effect is large. However, it is not the more the better, must be in a scienti c balance. Therefore, it is signi cant to the rapid detection of Zn 2+ in the environment and organism. Herein, a uorescent probe based on 2-hydroxy-1-naphthalene formaldehyde and furan-2-carbohydrazide was conveniently synthesized via Schiff base reaction. And this probe has been successfully applied to the accurate and quantitative detection of Zn 2+ in real samples, showing turn on uorescence, good selectivity, very low detection limit, real time response and reusability. In addition, this probe has the potential application to trace Zn 2+ in living cells with low cytotoxicity.