Electrical detection of cellular penetration during microinjection with carbon nanopipettes

Anderson, Seán T.; Bau, Haim H.

doi:10.1088/0957-4484/25/24/245102

Cited by 16 publications

(23 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1D. In contrast to our prior work, (29) the inner surface of the CNP is dry and not in contact with liquid. The symbols R and C denote, respectively, resistance and capacitance.…”

Section: Resultsmentioning

confidence: 82%

“…Microinjection experiments of fluorescent dye verified that this visual clue does, indeed, indicate cell penetration. (29) The measured impedance change occurred simultaneously with the imaged penetration into the cell cytoplasm or the cell nucleus. Nuclei were clearly visible under the microscope.…”

Section: Methodsmentioning

confidence: 98%

“…The CNP tip’s diameter, exposed length, and taper are controllable and determined by process conditions. To date, unfunctionalized CNPs have been used for cell (28, 29) and nematode (30) microinjection, cell electrophysiology (31) , fast-scan cyclic voltammetry to monitor neurotransmitter release and uptake in the brain of the fruit fly (32) , and scanning electrochemical microscopy. (33) The surface of the tip of the CNP has also been functionalized with gold nanoparticles (34) to enable the binding of various ligands and enhance surface Raman emission (35) .…”

Section: Introductionmentioning

confidence: 99%

“…(29) Cell penetration detection is useful, among other things, to trigger cell injection in automated injection systems. In the above application, the CNP was filled with the liquid to be injected.…”

Section: Introductionmentioning

confidence: 99%

“…(6, 7, 23–27, 32, 37) CNPs hold much promise for the above listed applications due to their small size, high spatial resolution, tunable dimensions, easy fabrication, and amenability to interfacing with standard electrophysiology and cell injection equipment. (3–5, 28, 29, 31) …”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Carbon nanoelectrodes for single-cell probing

Anderson¹,

Bau²

2015

Nanotechnology

Self Cite

View full text Add to dashboard Cite

Carbon nanoelectrodes with tip diameters ranging from tens to hundreds of nm are fabricated by pyrolitic deposition of carbon films along the entire inner surfaces of pulled-glass pipettes. The pulled end of each glass pipette is then etched to expose a desired length (typically, a few µm) of carbon pipe. The carbon film provides an electrically conductive path from the nanoscopic carbon tip to the distal, macroscopic end of the pipette, bridging between the nanoscale tip and the macroscale handle, without a need for assembly. We used our nanoelectrodes to penetrate into individual cells and cell nuclei and measured the variations in the electrode impedance upon cell and nucleus penetration as well as the electrode impedance as a function of cell penetration depth. Theoretical predictions based on a simple circuit model were in good agreement with experimental data.

show abstract

“…1D. In contrast to our prior work, (29) the inner surface of the CNP is dry and not in contact with liquid. The symbols R and C denote, respectively, resistance and capacitance.…”

Section: Resultsmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Carbon nanoelectrodes for single-cell probing

Anderson¹,

Bau²

2015

Nanotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

A Comprehensive Review on Intracellular Delivery

Rad

Bazaz

et al. 2021

Advanced Materials

View full text Add to dashboard Cite

Intracellular delivery is considered an indispensable process for various studies, ranging from medical applications (cell‐based therapy) to fundamental (genome‐editing) and industrial (biomanufacture) approaches. Conventional macroscale delivery systems critically suffer from such issues as low cell viability, cytotoxicity, and inconsistent material delivery, which have opened up an interest in the development of more efficient intracellular delivery systems. In line with the advances in microfluidics and nanotechnology, intracellular delivery based on micro‐ and nanoengineered platforms has progressed rapidly and held great promises owing to their unique features. These approaches have been advanced to introduce a smorgasbord of diverse cargoes into various cell types with the maximum efficiency and the highest precision. This review differentiates macro‐, micro‐, and nanoengineered approaches for intracellular delivery. The macroengineered delivery platforms are first summarized and then each method is categorized based on whether it employs a carrier‐ or membrane‐disruption‐mediated mechanism to load cargoes inside the cells. Second, particular emphasis is placed on the micro‐ and nanoengineered advances in the delivery of biomolecules inside the cells. Furthermore, the applications and challenges of the established and emerging delivery approaches are summarized. The topic is concluded by evaluating the future perspective of intracellular delivery toward the micro‐ and nanoengineered approaches.

show abstract

A Self‐contained Two‐electrode Nanosensor for Electrochemical Analysis in Aqueous Microenvironments

Scheibel

Schrlau

2020

Electroanalysis

View full text Add to dashboard Cite

We describe the development, fabrication, and characterization of a novel two‐electrode nanosensor contained within the tip of a needle‐like probe. This sensor consists of two, vertically aligned, carbon structures which function as individual electrodes. One of the carbon structures was modified by silver electrodeposition and chlorination to enable it to function as a pseudo‐reference electrode. Performance of this pseudo‐reference electrode was found to be comparable to that of commercially available Ag/AgCl reference electrodes. The unmodified carbon structure was employed as a working electrode versus the silver‐plated carbon structure to form a two‐electrode sensor capable of characterizing redox‐active analytes. The nanosensor was demonstrated to be capable of electrochemically characterizing the redox behavior of para‐aminophenol (PAP) in both bulk solutions and microenvironments. PAP was also measured in cell lysate to show that the nanosensor can detect small concentrations of analyte in heterogenous environments. As the working and reference electrodes are contained within a single nanoprobe, there was no requirement to position external electrodes within the electrochemical cell enabling analysis within very small domains. Due to the low‐cost manufacturing process, this nanoprobe has the potential to become a unique and widely accessible tool for the electrochemical characterization of microenvironments.

show abstract

Electrical detection of cellular penetration during microinjection with carbon nanopipettes

Cited by 16 publications

References 45 publications

Carbon nanoelectrodes for single-cell probing

Carbon nanoelectrodes for single-cell probing

A Comprehensive Review on Intracellular Delivery

A Self‐contained Two‐electrode Nanosensor for Electrochemical Analysis in Aqueous Microenvironments

Contact Info

Product

Resources

About